Detailed information NTG0432860

Bioinformatically predicted

Overview

• Name: pilC
• Type: Machinery gene
• Locus tag: G4G28_RS05485
• Genome accession: NZ_CP050454
• Coordinates: 1187448..1188683 (-)
• Length: 411 a.a.
• NCBI ID: WP_182991169.1
• Uniprot ID: A0A7G5ZBJ7
• Organism: Massilia sp. Dwa41.01b
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1182448..1193683

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
G4G28_RS05475 (G4G28_05470)	-	1183765..1185714 (-)	1950	WP_182991168.1	ATP-dependent endonuclease	-
G4G28_RS05480 (G4G28_05475)	-	1186155..1187299 (+)	1145	WP_182989678.1	IS3 family transposase	-
G4G28_RS05485 (G4G28_05480)	pilC	1187448..1188683 (-)	1236	WP_182991169.1	type II secretion system F family protein	Machinery gene
G4G28_RS05490 (G4G28_05485)	pilB	1188713..1190437 (-)	1725	WP_182991170.1	type IV-A pilus assembly ATPase PilB	Machinery gene
G4G28_RS05495 (G4G28_05490)	-	1190779..1192347 (-)	1569	WP_183108116.1	methyl-accepting chemotaxis protein	-
G4G28_RS05500 (G4G28_05495)	-	1192475..1192882 (-)	408	WP_182991172.1	hypothetical protein	-

Sequence

Protein

• Length: 411 a.a.
• Molecular weight: 44804.92 Da
• Isoelectric Point: 10.1211

>NTDB_id=432860 G4G28_RS05485 WP_182991169.1 1187448..1188683(-) (pilC) [Massilia sp. Dwa41.01b]
MATSLTTRARGGAQVKEAVYAWEGKDKSGKTVRGELRAGGEAIVNVTLRRQGIMVTKVKKKIYRSGKKITDKDLTMFTRQ
LATMMKAGVPLLQAFDIVGKGHANPSMSKLLLDIRGDIETGTSLNNAFRKYPRYFDPLFCNLVGAGEQAGILEDLLHRLA
IYKEKTLALKAKIKSAMTYPIAILAVAFIVVAVIMIWVVPAFKSVFTSFGADLPGPTLIVMGMSEFFVEWAWLIFPGIAA
AIYFFFQSWRRSLKMQQTMDRLLLRVPVFGDVIRKATIARWTRTLATMFAAGVPLVEALDSVGGASGNAVYLDATRRIQT
EVSTGTSLTVAMQNSNVFPSMVTQMVAIGEESGALDSMLGKVADFFEDEVDEAVAALSSLMEPMIMVILGVLIGGLVVAM
YLPIFKLGSVV

Nucleotide

• Length: 1236 bp

>NTDB_id=432860 G4G28_RS05485 WP_182991169.1 1187448..1188683(-) (pilC) [Massilia sp. Dwa41.01b]
ATGGCAACATCCCTCACCACCCGCGCCCGCGGCGGTGCCCAAGTCAAGGAAGCGGTCTACGCCTGGGAAGGCAAGGACAA
GTCCGGCAAGACCGTCCGTGGCGAACTGCGTGCCGGCGGCGAGGCGATCGTCAACGTGACCCTGCGCCGCCAGGGCATCA
TGGTCACCAAGGTCAAGAAGAAGATCTACCGCTCCGGCAAGAAGATCACCGACAAGGACCTGACCATGTTCACGCGCCAG
CTGGCAACGATGATGAAAGCCGGCGTGCCGCTGCTGCAGGCTTTCGATATCGTCGGCAAGGGCCACGCGAATCCGTCGAT
GTCCAAGCTCCTGCTCGACATCCGCGGCGATATCGAGACCGGTACCAGCCTGAACAACGCTTTCCGCAAGTACCCGCGCT
ATTTCGACCCGCTGTTCTGCAACCTGGTCGGCGCCGGCGAACAGGCCGGTATCCTGGAAGACCTGCTGCACCGCCTGGCG
ATCTACAAGGAAAAGACGCTGGCGCTGAAGGCCAAGATCAAGTCGGCCATGACCTACCCGATCGCCATTCTCGCCGTCGC
CTTCATCGTCGTGGCCGTGATCATGATCTGGGTGGTGCCGGCCTTCAAATCGGTGTTCACCAGTTTCGGCGCCGACCTCC
CGGGGCCGACCCTGATCGTGATGGGCATGTCGGAATTTTTCGTCGAATGGGCCTGGCTGATCTTCCCCGGCATTGCCGCC
GCCATCTACTTCTTCTTCCAGTCCTGGCGCCGCTCGCTCAAGATGCAGCAGACCATGGACCGCCTGCTGCTGCGCGTGCC
GGTCTTCGGCGACGTGATCCGCAAGGCCACCATTGCCCGCTGGACCCGTACGCTGGCGACCATGTTCGCCGCCGGCGTGC
CGCTGGTCGAGGCGCTCGATTCGGTGGGCGGGGCCTCCGGCAATGCCGTCTACCTCGACGCCACCCGCCGCATCCAGACC
GAAGTGTCGACCGGTACCAGCCTGACCGTGGCCATGCAGAACTCGAACGTCTTCCCCAGCATGGTGACCCAGATGGTGGC
GATCGGCGAGGAATCCGGCGCGCTCGACAGCATGCTGGGCAAGGTCGCCGACTTCTTCGAAGACGAGGTCGACGAAGCCG
TCGCCGCCCTCTCCTCGCTGATGGAACCGATGATCATGGTCATCCTGGGCGTGCTGATCGGCGGCCTGGTGGTGGCGATG
TACCTGCCGATCTTCAAGCTCGGTTCGGTGGTCTAA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein F (T2SSF) (281-403)

• Type II secretion system (T2SS), protein F (T2SSF) (77-200)

3D structure

• AlphaFold DB: A0A7G5ZBJ7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilC	Pseudomonas stutzeri DSM 10701	55.5	97.324	0.54
pilG	Neisseria gonorrhoeae MS11	53.652	96.594	0.518
pilC	Legionella pneumophila strain ERS1305867	53.535	96.35	0.516
pilG	Neisseria meningitidis 44/76-A	53.149	96.594	0.513
pilC	Acinetobacter baumannii D1279779	52.882	97.08	0.513
pilC	Acinetobacter baylyi ADP1	52.764	96.837	0.511
pilC	Vibrio cholerae strain A1552	41.414	96.35	0.399
pilC	Vibrio campbellii strain DS40M4	40.657	96.35	0.392
pilC	Thermus thermophilus HB27	38.346	97.08	0.372