Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   G4G28_RS05490 Genome accession   NZ_CP050454
Coordinates   1188713..1190437 (-) Length   574 a.a.
NCBI ID   WP_182991170.1    Uniprot ID   A0A7G5ZBJ8
Organism   Massilia sp. Dwa41.01b     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1183713..1195437
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G4G28_RS05475 (G4G28_05470) - 1183765..1185714 (-) 1950 WP_182991168.1 ATP-dependent endonuclease -
  G4G28_RS05480 (G4G28_05475) - 1186155..1187299 (+) 1145 WP_182989678.1 IS3 family transposase -
  G4G28_RS05485 (G4G28_05480) pilC 1187448..1188683 (-) 1236 WP_182991169.1 type II secretion system F family protein Machinery gene
  G4G28_RS05490 (G4G28_05485) pilB 1188713..1190437 (-) 1725 WP_182991170.1 type IV-A pilus assembly ATPase PilB Machinery gene
  G4G28_RS05495 (G4G28_05490) - 1190779..1192347 (-) 1569 WP_183108116.1 methyl-accepting chemotaxis protein -
  G4G28_RS05500 (G4G28_05495) - 1192475..1192882 (-) 408 WP_182991172.1 hypothetical protein -
  G4G28_RS05505 (G4G28_05500) - 1192893..1194149 (-) 1257 WP_182991173.1 hypothetical protein -
  G4G28_RS05510 (G4G28_05505) - 1194390..1194947 (-) 558 WP_182991174.1 ClpP family protease -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 61768.96 Da        Isoelectric Point: 4.8253

>NTDB_id=432861 G4G28_RS05490 WP_182991170.1 1188713..1190437(-) (pilB) [Massilia sp. Dwa41.01b]
MAAVLPNTTSSTPMSGLARALVQVGRLSAAQADACQKKAVADRSSFIDALLASGGIDAQSLAVFCAETFGYPLLDLSNFN
LASLPASAIDIKLMATQRVIALAKRGNRLSVAISDPTNTQGLDQIKFQSEAAVEPVIVAHDALLALLSSLTKSADESLGD
LAGDDNDIEFAEEEQSAVPVESTNDVEDAPIVRFLNKILLDAVQLGASDIHFEPFEKFYRIRLRVDGVLRDHAMPPLSIR
DKLVSRIKVLAKLDISEKRVPQDGRMRLILSATRTIDFRVSTLPTLFGEKTVMRILDATQAQMGIDALGYDPDQKALLLD
AISRPYGMVLVTGPTGSGKTVSLYSCLNVLNKPGINISTAEDPAEINLPGVNQVNVNDKAGLTFPVALKSFLRQDPDIIM
VGEIRDLETADIAIKAAQTGHMVFSTLHTNDAPSTLTRLMNMGVAPFNIASSVILITAQRLARRLCTCKQPVEMEPEALL
AAGYRESDLEGDWKPYGPVGCERCLGSGYKGRVGIYQIMPISEAIEALILANGNSMQIAEQAEKDGVNSLRRSGLMKVKQ
GLTSIEEVFGCTNE

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=432861 G4G28_RS05490 WP_182991170.1 1188713..1190437(-) (pilB) [Massilia sp. Dwa41.01b]
ATGGCAGCAGTCCTCCCGAACACGACCTCAAGCACACCGATGTCGGGTTTGGCGCGTGCGCTGGTGCAGGTCGGCCGTTT
GAGCGCGGCGCAAGCCGATGCTTGCCAAAAGAAAGCCGTCGCCGACCGCAGCAGCTTCATCGACGCACTCCTCGCCAGCG
GCGGCATCGATGCCCAGTCGCTCGCCGTGTTCTGCGCCGAGACCTTCGGCTATCCGCTGCTCGACCTGTCGAACTTCAAC
CTGGCTTCGCTGCCGGCCAGCGCGATCGACATCAAGCTGATGGCGACGCAACGCGTGATCGCCCTGGCCAAGCGCGGCAA
CCGACTCTCGGTCGCGATCTCCGATCCGACCAATACCCAGGGCCTGGACCAGATCAAGTTCCAGAGCGAAGCGGCAGTGG
AGCCGGTGATCGTGGCCCACGACGCCCTGCTGGCGCTCCTGTCGAGCCTGACCAAGTCCGCCGACGAGAGTCTGGGCGAC
CTGGCCGGCGACGACAACGACATCGAATTCGCCGAGGAAGAGCAAAGCGCGGTACCGGTGGAGTCCACCAACGATGTCGA
AGACGCGCCGATCGTGCGCTTCCTCAACAAAATCCTGCTGGACGCGGTGCAGCTGGGCGCTTCCGACATCCATTTCGAGC
CTTTCGAGAAGTTCTACCGCATCCGCTTGCGGGTCGACGGCGTGCTGCGCGACCATGCGATGCCGCCGCTGTCGATCCGC
GACAAGCTGGTCTCGCGCATCAAGGTGCTGGCCAAGCTCGACATCTCGGAAAAGCGCGTGCCGCAGGACGGGCGCATGCG
CCTGATCCTCTCAGCCACGCGCACCATCGACTTTCGCGTCAGCACCCTGCCCACGCTGTTCGGCGAAAAGACGGTCATGC
GTATCCTCGACGCGACCCAGGCCCAGATGGGCATCGACGCGCTCGGCTACGACCCCGACCAGAAGGCCCTGCTGCTCGAC
GCGATCTCGCGCCCTTACGGCATGGTGCTGGTGACCGGGCCGACCGGCTCGGGCAAGACCGTCTCGCTGTACAGCTGCCT
GAACGTGCTGAACAAGCCGGGCATCAATATCTCGACCGCGGAAGACCCGGCCGAGATCAACCTGCCCGGCGTGAACCAGG
TCAACGTCAACGACAAGGCCGGCCTCACCTTCCCGGTGGCGCTGAAGTCCTTCCTGCGCCAGGATCCCGACATCATCATG
GTCGGCGAGATCCGCGACCTGGAAACGGCCGACATCGCGATCAAGGCCGCGCAGACCGGCCACATGGTGTTCTCCACCCT
GCACACGAACGATGCGCCCTCGACCCTGACGCGCCTGATGAACATGGGCGTGGCCCCCTTCAACATCGCTTCCTCGGTGA
TCCTGATCACCGCGCAGCGCCTGGCACGGCGCCTGTGCACCTGCAAGCAGCCGGTCGAGATGGAACCCGAAGCGCTGCTG
GCCGCCGGCTACCGCGAGAGCGACCTGGAAGGCGACTGGAAGCCCTACGGCCCGGTGGGCTGCGAACGCTGCCTCGGCTC
CGGCTACAAGGGCCGCGTCGGCATCTACCAGATCATGCCGATCAGCGAGGCAATCGAGGCGCTGATCCTCGCGAACGGCA
ACTCGATGCAGATCGCCGAGCAGGCCGAGAAGGACGGCGTCAACTCGCTGCGCCGCTCGGGCCTGATGAAGGTGAAACAG
GGGCTGACGAGTATCGAGGAAGTATTTGGCTGTACCAACGAGTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-155)

Type II/IV secretion system protein (T2SSE)

(197-464)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7G5ZBJ8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

53.345

98.955

0.528
  pilF Neisseria gonorrhoeae MS11

53.357

98.606

0.526
  pilB Acinetobacter baumannii D1279779

52.531

99.826

0.524
  pilB Legionella pneumophila strain ERS1305867

48.592

98.955

0.481
  pilB Vibrio cholerae strain A1552

46.986

98.258

0.462
  pilB Vibrio parahaemolyticus RIMD 2210633

45.922

98.258

0.451
  pilB Vibrio campbellii strain DS40M4

45.31

98.432

0.446
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.146

97.909

0.383
  pilF Thermus thermophilus HB27

38.693

98.606

0.382