Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   HA248_RS10050 Genome accession   NZ_CP050175
Coordinates   1924804..1925745 (-) Length   313 a.a.
NCBI ID   WP_000249556.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain PZ900701590     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1919804..1930745
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HA248_RS10010 (HA248_09990) comYH 1920297..1921250 (-) 954 WP_000345119.1 class I SAM-dependent methyltransferase Machinery gene
  HA248_RS10015 (HA248_09995) - 1921311..1921898 (-) 588 WP_000679774.1 class I SAM-dependent methyltransferase -
  HA248_RS10020 (HA248_10000) comGG/cglG 1922035..1922448 (-) 414 WP_000265630.1 competence type IV pilus minor pilin ComGG Machinery gene
  HA248_RS10025 (HA248_10005) comGF/cglF 1922426..1922887 (-) 462 WP_000250545.1 competence type IV pilus minor pilin ComGF Machinery gene
  HA248_RS10030 (HA248_10010) comGE/cglE 1922850..1923152 (-) 303 WP_000413380.1 competence type IV pilus minor pilin ComGE Machinery gene
  HA248_RS10035 (HA248_10015) comGD/cglD 1923115..1923519 (-) 405 WP_000588005.1 competence type IV pilus minor pilin ComGD Machinery gene
  HA248_RS10040 (HA248_10020) comGC/cglC 1923512..1923838 (-) 327 WP_000738638.1 comG operon protein ComGC Machinery gene
  HA248_RS10045 (HA248_10025) comGB/cglB 1923840..1924856 (-) 1017 WP_013193332.1 competence type IV pilus assembly protein ComGB Machinery gene
  HA248_RS10050 (HA248_10030) comGA/cglA/cilD 1924804..1925745 (-) 942 WP_000249556.1 competence type IV pilus ATPase ComGA Machinery gene
  HA248_RS10055 (HA248_10035) - 1925821..1926186 (-) 366 WP_000286415.1 DUF1033 family protein -
  HA248_RS10060 (HA248_10040) - 1926337..1927395 (-) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -
  HA248_RS10065 (HA248_10045) nagA 1927558..1928709 (-) 1152 WP_033705218.1 N-acetylglucosamine-6-phosphate deacetylase -
  HA248_RS10070 (HA248_10050) - 1928862..1930679 (-) 1818 WP_001220843.1 acyltransferase family protein -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35534.46 Da        Isoelectric Point: 6.0083

>NTDB_id=430574 HA248_RS10050 WP_000249556.1 1924804..1925745(-) (comGA/cglA/cilD) [Streptococcus pneumoniae strain PZ900701590]
MVQEIAQEIIRSARKKGAQDIYFVPKLDAYELHMRVGDERCKIGSYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYA
YDQKMASLRLSTVGDYRGHESLVIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFASRDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=430574 HA248_RS10050 WP_000249556.1 1924804..1925745(-) (comGA/cglA/cilD) [Streptococcus pneumoniae strain PZ900701590]
ATGGTTCAAGAAATTGCACAAGAAATCATTCGTTCGGCTCGGAAAAAAGGGGCGCAAGACATTTATTTTGTCCCTAAGTT
AGATGCCTATGAGCTTCATATGAGGGTAGGAGACGAGCGCTGTAAAATTGGTAGCTATGATTTTGAAAAGTTTGCAGCCG
TTATCAGTCACTTTAAGTTTGTGGCGGGTATGAATGTGGGAGAAAAAAGACGTAGTCAACTGGGTTCCTGTGATTATGCC
TATGACCAGAAGATGGCGTCTCTACGTTTATCTACTGTAGGCGATTATCGGGGGCATGAGAGTTTGGTTATCCGTTTGTT
GCACGATGAGGAGCAGGACTTGCATTTTTGGTTTCAGGATATTGAAGAATTAGGCAAGCAGTACAGGCAACGGGGACTCT
ATCTTTTTGCTGGTCCGGTTGGGAGTGGTAAGACGACCTTGATGCATGAATTGTCCAAGTCACTCTTTAAAGGACAGCAA
GTTATGTCCATCGAAGATCCTGTCGAAATCAAGCAGGACGACATGCTTCAGTTGCAGTTGAACGAAGCAATCGGCCTAAC
CTATGAAAATCTAATCAAACTTTCCTTGCGTCATCGACCAGATCTCTTGATTATCGGAGAAATTCGTGACAGCGAGACGG
CGCGTGCAGTGGTCAGAGCTAGTTTGACAGGTGCGACAGTCTTTTCAACCATTCACGCCAAAAGTATCCGAGGTGTTTAT
GAGCGTCTGCTGGAGTTGGGTGTGAGTGAAGAAGAATTGGCAGTTGTTCTGCAAGGAGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCAAGCAGAGATTATCAAGAACACCAAGCAGCCAAGTGGAATGAGCAAATTGACCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

99.042

100

0.99
  comGA/cglA/cilD Streptococcus pneumoniae D39

99.042

100

0.99
  comGA/cglA/cilD Streptococcus pneumoniae R6

99.042

100

0.99
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

99.042

100

0.99
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

96.805

100

0.968
  comYA Streptococcus gordonii str. Challis substr. CH1

78.387

99.042

0.776
  comYA Streptococcus mutans UA159

66.238

99.361

0.658
  comYA Streptococcus mutans UA140

66.238

99.361

0.658
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.903

99.042

0.623
  comGA Lactococcus lactis subsp. cremoris KW2

55.128

99.681

0.55
  comGA Latilactobacillus sakei subsp. sakei 23K

41.912

86.901

0.364