Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   IFJ80_RS25400 Genome accession   NZ_CP061777
Coordinates   5393017..5393484 (-) Length   155 a.a.
NCBI ID   WP_003161762.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain ZBX-P23     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5388017..5398484
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IFJ80_RS25380 (IFJ80_25375) nadC 5389401..5390249 (+) 849 WP_003161760.1 carboxylating nicotinate-nucleotide diphosphorylase -
  IFJ80_RS25390 (IFJ80_25385) - 5390430..5391641 (-) 1212 WP_249225081.1 O-antigen ligase family protein -
  IFJ80_RS25395 (IFJ80_25390) - 5391670..5392698 (+) 1029 WP_050161940.1 IS30-like element ISPsp8 family transposase -
  IFJ80_RS25400 (IFJ80_25395) pilA/pilAI 5393017..5393484 (-) 468 WP_003161762.1 pilin Machinery gene
  IFJ80_RS25405 (IFJ80_25400) pilB 5393715..5395415 (+) 1701 WP_003122078.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IFJ80_RS25410 (IFJ80_25405) pilC 5395419..5396636 (+) 1218 WP_003161763.1 type II secretion system F family protein Machinery gene
  IFJ80_RS25415 (IFJ80_25410) pilD 5396637..5397509 (+) 873 WP_003161765.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  IFJ80_RS25420 (IFJ80_25415) coaE 5397506..5398117 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  IFJ80_RS25425 (IFJ80_25420) yacG 5398114..5398314 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 15959.30 Da        Isoelectric Point: 9.0145

>NTDB_id=422608 IFJ80_RS25400 WP_003161762.1 5393017..5393484(-) (pilA/pilAI) [Pseudomonas aeruginosa strain ZBX-P23]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQDYTARTQVTRAVSEISALKTAAESAILEGKKLVSKDNPADGEYDLGFTK
STLLAGNDGKAQITITGESSATPTIAGTLGNSAGKAISGAVITIKRSAEGVWTCAISGSPANWKANYAPANCPKS

Nucleotide


Download         Length: 468 bp        

>NTDB_id=422608 IFJ80_RS25400 WP_003161762.1 5393017..5393484(-) (pilA/pilAI) [Pseudomonas aeruginosa strain ZBX-P23]
ATGAAAGCTCAGAAGGGTTTTACTCTGATCGAACTGATGATCGTGGTCGCGATCATCGGCATCCTGGCCGCCATTGCCAT
CCCGCAATACCAGGACTACACCGCCCGTACCCAGGTGACCCGTGCCGTGAGTGAAATCAGCGCGTTGAAGACCGCTGCGG
AGTCGGCGATTCTGGAAGGCAAGAAGCTTGTTTCCAAGGATAATCCCGCGGATGGGGAATATGATCTTGGTTTTACCAAG
TCTACTTTGCTTGCTGGCAACGACGGTAAGGCACAGATCACCATCACTGGCGAAAGCAGTGCAACCCCGACCATTGCGGG
GACTCTGGGTAACTCTGCTGGTAAGGCCATCAGCGGTGCCGTTATCACCATCAAGCGTAGTGCTGAGGGAGTCTGGACCT
GCGCTATCAGTGGGTCTCCGGCCAACTGGAAAGCCAACTACGCTCCGGCCAACTGCCCGAAATCCTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

45.679

100

0.477
  pilA Pseudomonas aeruginosa PAK

40.964

100

0.439
  pilA Acinetobacter baumannii strain A118

43.312

100

0.439
  pilA Ralstonia pseudosolanacearum GMI1000

37.427

100

0.413
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

43.262

90.968

0.394
  pilA Vibrio cholerae strain A1552

43.262

90.968

0.394
  pilA Vibrio cholerae C6706

43.262

90.968

0.394
  pilA/pilAII Pseudomonas stutzeri DSM 10701

39.333

96.774

0.381
  pilA/pilA1 Eikenella corrodens VA1

38.095

94.839

0.361