Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   G3U99_RS06590 Genome accession   NZ_CP048693
Coordinates   1356848..1358494 (-) Length   548 a.a.
NCBI ID   WP_165579506.1    Uniprot ID   -
Organism   Vibrio coralliilyticus OCN008     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1351848..1363494
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G3U99_RS06560 (G3U99_06535) rplS 1352477..1352830 (+) 354 WP_006958143.1 50S ribosomal protein L19 -
  G3U99_RS06565 (G3U99_06540) yacG 1353076..1353270 (-) 195 WP_019277521.1 DNA gyrase inhibitor YacG -
  G3U99_RS06570 (G3U99_06545) zapD 1353322..1354062 (-) 741 WP_021458283.1 cell division protein ZapD -
  G3U99_RS06575 (G3U99_06550) coaE 1354096..1354704 (-) 609 WP_021458284.1 dephospho-CoA kinase -
  G3U99_RS06580 (G3U99_06555) pilD 1354706..1355575 (-) 870 WP_021458285.1 prepilin peptidase Machinery gene
  G3U99_RS06585 (G3U99_06560) pilC 1355625..1356839 (-) 1215 WP_031343345.1 type II secretion system F family protein Machinery gene
  G3U99_RS06590 (G3U99_06565) pilB 1356848..1358494 (-) 1647 WP_165579506.1 type IV-A pilus assembly ATPase PilB Machinery gene
  G3U99_RS06595 (G3U99_06570) - 1358497..1358937 (-) 441 WP_031343346.1 pilin -
  G3U99_RS06600 (G3U99_06575) nadC 1359165..1360052 (-) 888 WP_021458291.1 carboxylating nicotinate-nucleotide diphosphorylase -
  G3U99_RS06605 (G3U99_06580) ampD 1360184..1360729 (+) 546 WP_006958131.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  G3U99_RS06610 (G3U99_06585) fldB 1360857..1361378 (-) 522 WP_031343347.1 flavodoxin FldB -
  G3U99_RS06615 (G3U99_06590) xerD 1361542..1362438 (+) 897 WP_031343348.1 site-specific tyrosine recombinase XerD -
  G3U99_RS06620 (G3U99_06595) - 1362478..1363230 (+) 753 WP_021458293.1 thioredoxin fold domain-containing protein -

Sequence

Protein


Download         Length: 548 a.a.        Molecular weight: 60731.08 Da        Isoelectric Point: 4.7969

>NTDB_id=422400 G3U99_RS06590 WP_165579506.1 1356848..1358494(-) (pilB) [Vibrio coralliilyticus OCN008]
MSELPFLLKKANKISEEQALQAISATETRQIEAYSALLEISDLTSKQLTAAISELYALPIVSLDDFDYRTCCEQLGIGDL
IRSHTALPLEVERQHLTLALADPSISEIEQEFRFATGLAIKLVLAELPTLARAINNLYGAQSASDSLGYKEVSVLDLEQL
VEAEEHELIDSEDLNHDSSPISRYIQQILLDAVRKQASDIHFEPHESWFRVRFRCDGLLVEVQRPPQHLCQRLTARLKIL
AKLDIAERRLPQDGRITLNVTQTDSIDIRVSTLPTIWGEKIVLRLLDNRMTSFDLSSLGYNHIQSESYRQALTKPQGLIL
ITGPTGSGKTLSLYSGLNIINQNEINIATAEDPVEIHLTGINQVQIQPQIGFDFAQALRAFLRQDPDVVMVGEIRDRETA
EIATKAAQTGHLVLSTLHTNSAAEAILRLQNMGIEAFNLAASLSLIIAQRLVRCLCTQCKRPTRARTGQYEASSEGCQDC
NQGYSGRTGIYEVLTVTPEIAQAINDGATANQIEDLAIQQGMQTLPHSGLEKLAQGITSQAELQRMLF

Nucleotide


Download         Length: 1647 bp        

>NTDB_id=422400 G3U99_RS06590 WP_165579506.1 1356848..1358494(-) (pilB) [Vibrio coralliilyticus OCN008]
ATGAGTGAACTGCCCTTCTTGCTAAAAAAAGCCAATAAAATTAGTGAAGAACAAGCCCTGCAAGCAATATCAGCTACAGA
AACCCGACAAATTGAGGCGTATTCTGCTCTACTAGAGATTAGTGACCTGACATCCAAACAGCTGACAGCAGCTATCAGTG
AGCTCTATGCTCTCCCCATCGTGTCGTTAGATGACTTCGATTATCGAACTTGTTGCGAGCAGCTCGGCATTGGCGACTTA
ATTCGCAGCCACACGGCACTGCCACTAGAAGTAGAGCGACAACATCTTACTCTAGCGCTCGCCGATCCTTCCATTTCCGA
GATAGAACAAGAATTTCGTTTTGCAACTGGCCTAGCAATCAAACTCGTATTGGCCGAGTTACCGACTTTAGCCCGTGCGA
TAAACAACCTATATGGAGCTCAAAGCGCCAGTGACTCTTTAGGCTACAAAGAGGTTAGCGTCCTAGACCTTGAACAATTA
GTCGAAGCTGAAGAACATGAATTAATAGACAGTGAGGATCTCAATCACGATAGCTCGCCAATCAGTCGCTATATCCAGCA
GATACTGCTGGATGCCGTACGTAAGCAAGCTTCTGATATCCATTTTGAACCTCATGAATCATGGTTTCGCGTCCGGTTTC
GTTGTGATGGCTTGCTTGTTGAAGTGCAGCGACCTCCTCAGCACCTATGCCAAAGGTTAACCGCCAGACTCAAAATCCTC
GCTAAGCTGGACATCGCCGAGCGCAGACTCCCGCAAGATGGTCGTATCACTCTCAATGTGACTCAAACTGATTCTATCGA
CATACGTGTATCGACTCTGCCTACCATATGGGGTGAAAAAATTGTCCTTAGATTGCTCGACAACAGAATGACCAGCTTTG
ACTTAAGCAGCCTTGGCTACAATCACATTCAAAGCGAAAGCTATCGTCAGGCGCTAACCAAGCCTCAGGGACTAATTCTA
ATCACTGGCCCCACGGGAAGCGGTAAGACTCTGTCTTTATACAGTGGACTTAACATCATCAATCAGAATGAGATCAACAT
CGCGACGGCAGAGGACCCCGTAGAGATTCACCTAACAGGAATCAATCAGGTGCAAATTCAACCTCAAATCGGCTTTGATT
TCGCTCAGGCACTCAGAGCCTTTCTACGTCAGGATCCCGATGTGGTTATGGTGGGTGAAATTCGCGATCGAGAAACTGCC
GAGATAGCCACCAAAGCGGCTCAAACAGGCCATTTAGTCCTTTCAACACTCCATACCAACTCAGCAGCAGAAGCTATTCT
TCGCCTCCAGAATATGGGAATAGAAGCTTTCAACCTTGCTGCTTCTCTTAGCCTCATCATTGCCCAGCGTTTAGTCAGAT
GCCTATGCACTCAATGCAAGCGCCCCACGAGAGCCCGGACAGGTCAATATGAGGCTTCTTCTGAAGGTTGTCAGGACTGC
AACCAAGGCTACTCAGGACGAACTGGTATTTATGAAGTACTCACAGTGACACCTGAAATTGCGCAAGCTATTAACGACGG
CGCCACAGCTAACCAGATTGAGGACTTGGCGATACAACAAGGCATGCAAACATTACCGCACTCGGGCTTAGAAAAATTGG
CCCAAGGTATCACCAGTCAGGCAGAACTGCAGCGAATGCTATTCTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-142)

Type II/IV secretion system protein (T2SSE)

(187-453)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

56.429

100

0.577
  pilB Vibrio campbellii strain DS40M4

55.814

100

0.569
  pilB Vibrio parahaemolyticus RIMD 2210633

55.735

100

0.568
  pilB Legionella pneumophila strain ERS1305867

46.654

95.438

0.445
  pilB Acinetobacter baumannii D1279779

44.547

98.723

0.44
  pilB Acinetobacter baylyi ADP1

45.627

95.985

0.438
  pilF Neisseria gonorrhoeae MS11

41.843

95.073

0.398
  pilB Haemophilus influenzae Rd KW20

44.231

85.401

0.378
  pilF Thermus thermophilus HB27

38.899

96.168

0.374
  pilB Haemophilus influenzae 86-028NP

43.376

85.401

0.37

Multiple sequence alignment