Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   G3U99_RS05265 Genome accession   NZ_CP048693
Coordinates   1113486..1114802 (+) Length   438 a.a.
NCBI ID   WP_242704147.1    Uniprot ID   -
Organism   Vibrio coralliilyticus OCN008     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1108486..1119802
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G3U99_RS05245 (G3U99_05230) pilM 1110876..1111793 (+) 918 WP_021458428.1 pilus assembly protein PilM -
  G3U99_RS05250 (G3U99_05235) - 1111783..1112340 (+) 558 WP_021458427.1 PilN domain-containing protein -
  G3U99_RS05255 (G3U99_05240) pilO 1112333..1112923 (+) 591 WP_021458426.1 type 4a pilus biogenesis protein PilO -
  G3U99_RS05260 (G3U99_05245) - 1112913..1113425 (+) 513 WP_021458425.1 pilus assembly protein PilP -
  G3U99_RS05265 (G3U99_05250) pilQ 1113486..1114802 (+) 1317 WP_242704147.1 type IV pilus secretin PilQ Machinery gene
  G3U99_RS05270 (G3U99_05255) aroK 1114979..1115497 (+) 519 WP_006957155.1 shikimate kinase AroK -
  G3U99_RS05275 (G3U99_05260) aroB 1115529..1116617 (+) 1089 WP_021458026.1 3-dehydroquinate synthase -
  G3U99_RS05280 (G3U99_05265) - 1116659..1118159 (+) 1501 Protein_978 SPOR domain-containing protein -
  G3U99_RS05285 (G3U99_05270) - 1118229..1119056 (+) 828 WP_006957149.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 438 a.a.        Molecular weight: 47460.16 Da        Isoelectric Point: 4.5486

>NTDB_id=422391 G3U99_RS05265 WP_242704147.1 1113486..1114802(+) (pilQ) [Vibrio coralliilyticus OCN008]
MVTHNAASSEEMKPELEIEGKVSDKLSVNFQEIPVRQVLQQIADYNDFNLVVSDAVSGNLTLRVKEVPWPQLLDIILRMK
GLEKRMDGNVLLVATAAEIAQFKQQKLEQEKVTAELQPLESEIIAMRFAKAADIAALLSDSAATRMLSERGAISIDTRTN
SLLIRDVAINIQAMKAIITALDVPVKQVQIEARIVSLNEGDLQELGVRWGFSSTNGSHSVGGSIENNLANLNLYGDSDSS
LDDFLNVNLAATSVNASTIAFQVAKLGSDTLLDLELSALQRESRAEVISSPRLITTNKQLAYIEQGTEIPYLEAASSGAT
SVNFKKAVLSLKVTPQITSDNRLILDLNVTQDRPGQVVKTGTGEAVAIDTQRIGTQVLVNNGETIVLGGIYQHSVSDTVD
QVPLLGDIPLLGALFRRTYQQMGKSELLIFVTPTVVAP

Nucleotide


Download         Length: 1317 bp        

>NTDB_id=422391 G3U99_RS05265 WP_242704147.1 1113486..1114802(+) (pilQ) [Vibrio coralliilyticus OCN008]
ATGGTTACGCATAATGCAGCAAGCAGTGAGGAGATGAAGCCTGAACTTGAGATAGAGGGAAAGGTGTCAGACAAACTTTC
CGTCAATTTTCAGGAGATTCCGGTGCGCCAGGTATTGCAGCAGATTGCGGATTACAACGATTTTAATCTGGTTGTGTCAG
ATGCCGTGTCTGGCAATCTGACCCTAAGAGTGAAGGAGGTTCCGTGGCCACAGTTACTCGATATCATTTTGCGTATGAAG
GGATTAGAGAAGCGCATGGATGGCAATGTGTTGCTGGTGGCAACGGCGGCTGAAATTGCTCAGTTCAAACAACAAAAGTT
GGAACAGGAGAAAGTCACGGCTGAGCTTCAACCGTTAGAATCTGAGATTATCGCTATGAGGTTTGCCAAAGCGGCAGATA
TTGCGGCATTACTGAGCGATAGCGCTGCGACGCGGATGTTGTCTGAACGAGGGGCGATTTCAATCGATACTCGAACCAAT
AGCCTGTTAATTCGCGATGTTGCTATCAATATTCAAGCGATGAAGGCGATCATTACGGCATTAGATGTGCCGGTGAAACA
AGTACAAATTGAGGCGCGCATAGTCAGCTTGAATGAAGGTGATTTACAGGAGTTGGGTGTGCGTTGGGGGTTCAGTTCGA
CCAACGGCAGTCACAGTGTGGGAGGCTCGATAGAAAATAACTTAGCCAATCTCAATCTGTATGGGGATTCGGACTCGTCA
CTGGACGATTTTCTCAACGTCAACTTAGCGGCGACGTCCGTGAACGCTTCGACGATTGCTTTTCAGGTCGCTAAGCTGGG
CAGTGACACTTTACTGGATTTAGAATTGTCAGCGTTGCAAAGGGAATCGAGAGCAGAAGTTATATCCAGTCCGCGTCTGA
TTACCACCAACAAGCAACTGGCTTACATTGAGCAGGGGACTGAAATTCCTTATTTAGAGGCGGCCTCAAGCGGTGCAACC
AGCGTGAATTTTAAAAAAGCAGTGTTAAGTTTAAAAGTCACCCCGCAAATCACATCGGATAACCGTTTGATTCTGGATCT
GAATGTCACCCAAGATAGGCCGGGTCAAGTGGTCAAAACCGGCACTGGGGAAGCTGTTGCGATAGACACGCAACGTATCG
GTACTCAGGTACTGGTCAATAATGGCGAAACCATTGTTCTGGGAGGCATTTATCAGCACAGTGTTTCGGATACGGTCGAT
CAGGTTCCGTTGTTAGGCGACATTCCTTTGCTAGGTGCGCTTTTTCGACGAACTTATCAGCAAATGGGTAAGAGCGAGTT
GTTGATTTTTGTAACGCCAACAGTGGTTGCGCCTTAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(122-187)

Bacterial type II and III secretion system protein (Secretin)

(278-436)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

69.406

100

0.694
  pilQ Vibrio cholerae strain A1552

69.406

100

0.694
  pilQ Vibrio campbellii strain DS40M4

70.629

97.945

0.692
  pilQ Legionella pneumophila strain ERS1305867

46.89

95.434

0.447
  pilQ Legionella pneumophila strain Lp02

46.89

95.434

0.447
  comQ Acinetobacter baylyi ADP1

42.664

100

0.432
  pilQ Pseudomonas aeruginosa PAK

43.721

98.174

0.429
  pilQ Acinetobacter baumannii D1279779

39.168

100

0.409
  pilQ Acinetobacter baumannii strain A118

38.731

100

0.404
  comE Haemophilus influenzae 86-028NP

41.748

94.064

0.393
  comE Haemophilus influenzae Rd KW20

41.007

95.205

0.39

Multiple sequence alignment