Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   G3T13_RS09885 Genome accession   NZ_CP048686
Coordinates   2007761..2008702 (+) Length   313 a.a.
NCBI ID   WP_061774317.1    Uniprot ID   -
Organism   Lactococcus raffinolactis strain KACC 13441     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2002761..2013702
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G3T13_RS09875 - 2005495..2005857 (+) 363 WP_061774315.1 hypothetical protein -
  G3T13_RS09880 - 2005854..2007698 (+) 1845 WP_061774316.1 acyltransferase family protein -
  G3T13_RS09885 comGA/cglA/cilD 2007761..2008702 (+) 942 WP_061774317.1 competence type IV pilus ATPase ComGA Machinery gene
  G3T13_RS09890 comYB 2008584..2009669 (+) 1086 WP_306301678.1 competence type IV pilus assembly protein ComGB Machinery gene
  G3T13_RS09895 comYC 2009693..2009998 (+) 306 WP_174519819.1 competence type IV pilus major pilin ComGC Machinery gene
  G3T13_RS11680 comGD 2009949..2010398 (+) 450 WP_275575940.1 competence type IV pilus minor pilin ComGD -
  G3T13_RS09905 comGE 2010370..2010675 (+) 306 WP_082785376.1 competence type IV pilus minor pilin ComGE -
  G3T13_RS09910 - 2010824..2011093 (+) 270 WP_239519114.1 competence type IV pilus minor pilin ComGF -
  G3T13_RS09915 comGG 2011105..2011389 (+) 285 WP_061774321.1 competence type IV pilus minor pilin ComGG -
  G3T13_RS09920 ispE 2011394..2012245 (+) 852 WP_061774322.1 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase -
  G3T13_RS09925 - 2012329..2013177 (+) 849 WP_061774323.1 aminotransferase class IV -
  G3T13_RS09930 - 2013253..2013702 (+) 450 WP_061774324.1 zinc-dependent MarR family transcriptional regulator -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 34933.21 Da        Isoelectric Point: 9.1747

>NTDB_id=422297 G3T13_RS09885 WP_061774317.1 2007761..2008702(+) (comGA/cglA/cilD) [Lactococcus raffinolactis strain KACC 13441]
MIKEIAQRMLKKASDFGASDIYILPARTGFSVVFRKSAHREYDQLLSDAEGQSLISHFKFTAGMNVGEKRRPQLGSCLYE
LADRKCRLRLSSAGDFESRESLVIRILHDTKQPLKFWIEADLPQVKKLVARRGLYLFAGPVGSGKTTLMHHIAKEKFSGQ
QVITIEDPVEIVAPDLLQFQLNEAIGNTYDSLIKLSLRHMPDLVIVGEIRDQETARAVMRASLTGYTVFSTIHAKSIAGV
YARLLELGVTKEEINNSLSGVVYQRLIAGKGVLDSAEKAFERHANDKWHAKIEKLVTEGHLTPDQATAEKVSD

Nucleotide


Download         Length: 942 bp        

>NTDB_id=422297 G3T13_RS09885 WP_061774317.1 2007761..2008702(+) (comGA/cglA/cilD) [Lactococcus raffinolactis strain KACC 13441]
ATGATAAAGGAAATAGCGCAAAGGATGTTAAAGAAAGCAAGCGATTTTGGGGCAAGTGATATTTATATATTACCCGCCAG
AACAGGATTTTCGGTTGTTTTCAGAAAGTCAGCACATCGTGAATATGATCAGCTCCTGTCAGATGCTGAAGGTCAAAGTC
TCATTTCCCATTTCAAATTTACAGCAGGGATGAATGTAGGTGAGAAGCGCCGGCCACAACTAGGATCTTGCTTGTATGAG
CTTGCAGATAGAAAGTGTCGCTTGCGTCTATCCTCAGCGGGTGACTTTGAAAGTCGCGAAAGTTTGGTGATTCGAATTTT
GCATGACACGAAACAACCACTCAAGTTTTGGATTGAGGCAGATTTACCTCAGGTAAAAAAGTTAGTAGCTAGACGCGGTC
TTTATTTATTTGCGGGGCCAGTTGGTTCTGGGAAAACGACGCTGATGCATCACATTGCCAAAGAAAAGTTTTCTGGTCAG
CAAGTCATTACGATTGAAGATCCAGTTGAAATCGTTGCACCAGATCTGCTCCAATTCCAACTGAATGAAGCGATTGGCAA
CACCTATGATAGCTTGATTAAATTATCCTTGCGCCATATGCCAGATCTTGTGATTGTCGGAGAAATTCGAGATCAGGAAA
CTGCGCGAGCAGTCATGAGGGCCAGTCTGACGGGCTATACTGTTTTTTCGACGATTCATGCTAAGTCGATTGCGGGTGTT
TATGCGCGATTATTGGAGCTTGGCGTAACAAAAGAAGAAATCAATAATTCGTTGTCAGGTGTTGTCTATCAACGGTTGAT
TGCAGGAAAGGGGGTGCTAGATAGTGCTGAAAAAGCATTCGAAAGGCATGCCAACGACAAGTGGCATGCCAAAATTGAAA
AATTGGTTACAGAAGGACATCTCACACCTGATCAGGCGACAGCCGAAAAGGTTAGCGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

57.692

99.681

0.575
  comGA/cglA/cilD Streptococcus pneumoniae R6

56.731

99.681

0.565
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

56.731

99.681

0.565
  comGA/cglA/cilD Streptococcus pneumoniae D39

56.731

99.681

0.565
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

56.731

99.681

0.565
  comGA Lactococcus lactis subsp. cremoris KW2

56.23

100

0.562
  comYA Streptococcus gordonii str. Challis substr. CH1

55.911

100

0.559
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

53.674

100

0.537
  comYA Streptococcus mutans UA140

52.548

100

0.527
  comYA Streptococcus mutans UA159

52.548

100

0.527

Multiple sequence alignment