Detailed information    

insolico Bioinformatically predicted

Overview

Name   ssbA   Type   Machinery gene
Locus tag   G3T13_RS07625 Genome accession   NZ_CP048686
Coordinates   1581023..1581418 (-) Length   131 a.a.
NCBI ID   WP_061773653.1    Uniprot ID   -
Organism   Lactococcus raffinolactis strain KACC 13441     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1574772..1614713 1581023..1581418 within 0


Gene organization within MGE regions


Location: 1574772..1614713
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G3T13_RS07595 - 1574772..1576064 (-) 1293 WP_061773660.1 adenylosuccinate synthase -
  G3T13_RS07600 - 1576547..1577065 (+) 519 WP_061773659.1 hypothetical protein -
  G3T13_RS07605 - 1577210..1577470 (-) 261 WP_061773658.1 SemiSWEET family transporter -
  G3T13_RS07610 groL 1577673..1579304 (-) 1632 WP_061773657.1 chaperonin GroEL -
  G3T13_RS07615 groES 1579372..1579656 (-) 285 WP_061773656.1 co-chaperone GroES -
  G3T13_RS11480 - 1579832..1580395 (-) 564 WP_061773655.1 hypothetical protein -
  G3T13_RS11485 - 1580432..1580989 (-) 558 WP_061773654.1 CHAP domain-containing protein -
  G3T13_RS07625 ssbA 1581023..1581418 (-) 396 WP_061773653.1 single-stranded DNA-binding protein Machinery gene
  G3T13_RS07630 ytpR 1581490..1582113 (-) 624 WP_061773652.1 YtpR family tRNA-binding protein -
  G3T13_RS07635 - 1582143..1582460 (-) 318 WP_061773651.1 thioredoxin family protein -
  G3T13_RS07640 - 1582473..1582772 (-) 300 WP_061773650.1 hypothetical protein -
  G3T13_RS07645 pepA 1582911..1583996 (+) 1086 WP_061773649.1 glutamyl aminopeptidase -
  G3T13_RS07650 - 1584050..1586974 (-) 2925 WP_061773648.1 glucosaminidase domain-containing protein -
  G3T13_RS07655 - 1587168..1589672 (-) 2505 WP_144259867.1 GH25 family lysozyme -
  G3T13_RS07660 - 1589858..1590214 (-) 357 WP_061773646.1 hypothetical protein -
  G3T13_RS07665 - 1590227..1592170 (-) 1944 WP_061773645.1 Ig-like domain-containing protein -
  G3T13_RS07670 - 1592322..1593608 (-) 1287 WP_061773644.1 hypothetical protein -
  G3T13_RS07675 - 1593675..1594610 (-) 936 WP_061773643.1 glycosyltransferase family 2 protein -
  G3T13_RS07680 - 1594930..1596096 (-) 1167 WP_061773642.1 nucleotide sugar dehydrogenase -
  G3T13_RS07685 - 1596147..1597067 (-) 921 WP_061773641.1 hypothetical protein -
  G3T13_RS07690 - 1597067..1598476 (-) 1410 WP_061773640.1 lipopolysaccharide biosynthesis protein -
  G3T13_RS07695 - 1598463..1599467 (-) 1005 WP_061773639.1 NAD-dependent epimerase -
  G3T13_RS07700 - 1599478..1600464 (-) 987 WP_061773638.1 alpha-1,2-fucosyltransferase -
  G3T13_RS07705 - 1600466..1601539 (-) 1074 WP_163604802.1 glycosyltransferase -
  G3T13_RS07710 - 1601574..1602548 (-) 975 WP_061773636.1 glycosyltransferase family 2 protein -
  G3T13_RS07715 - 1602545..1603498 (-) 954 WP_082785326.1 glycosyltransferase family 2 protein -
  G3T13_RS07720 - 1603532..1603900 (-) 369 WP_061773634.1 DUF2304 domain-containing protein -
  G3T13_RS07725 - 1603903..1604628 (-) 726 WP_061773633.1 glycosyltransferase family 2 protein -
  G3T13_RS07730 - 1604760..1605710 (-) 951 WP_061773632.1 NAD-dependent epimerase/dehydratase family protein -
  G3T13_RS07735 - 1605723..1606508 (-) 786 WP_061773631.1 glycosyltransferase family 2 protein -
  G3T13_RS07740 - 1606549..1607934 (-) 1386 WP_061773630.1 DUF2142 domain-containing protein -
  G3T13_RS07745 - 1607994..1609688 (-) 1695 WP_061773795.1 rhamnan synthesis F family protein -
  G3T13_RS07750 - 1609688..1610656 (-) 969 WP_061773629.1 glycosyltransferase family 2 protein -
  G3T13_RS07755 - 1610658..1611860 (-) 1203 WP_061773628.1 ABC transporter ATP-binding protein -
  G3T13_RS07760 - 1611861..1612649 (-) 789 WP_061773627.1 ABC transporter permease -
  G3T13_RS07765 - 1612646..1613581 (-) 936 WP_061773626.1 glycosyltransferase family 2 protein -
  G3T13_RS07770 cps2T 1613571..1614713 (-) 1143 WP_061773625.1 beta 1-4 rhamnosyltransferase Cps2T -

Sequence

Protein


Download         Length: 131 a.a.        Molecular weight: 14732.86 Da        Isoelectric Point: 6.2377

>NTDB_id=422291 G3T13_RS07625 WP_061773653.1 1581023..1581418(-) (ssbA) [Lactococcus raffinolactis strain KACC 13441]
MLNKSLLIGRLTAQPEVKKTPTDKSVLRVTLAVSRQFKNADGQRDADFLTIIIWGKPAELFASYGKKGSLVSIEGELRTH
RYEDKAGVTHYVTEVLCHQFNLLESRAAVALRENQVETVTDVMLEDEDLPF

Nucleotide


Download         Length: 396 bp        

>NTDB_id=422291 G3T13_RS07625 WP_061773653.1 1581023..1581418(-) (ssbA) [Lactococcus raffinolactis strain KACC 13441]
ATGTTAAATAAAAGTTTACTAATTGGTCGTTTGACCGCACAGCCGGAAGTGAAGAAAACACCAACGGATAAATCTGTCTT
GCGCGTGACCTTAGCTGTTAGCCGACAATTTAAAAACGCTGATGGGCAACGGGATGCAGATTTTTTGACCATTATCATTT
GGGGCAAACCCGCAGAGCTATTTGCATCCTACGGTAAAAAAGGCAGTCTAGTTTCGATTGAGGGAGAACTCCGAACGCAT
CGTTACGAGGACAAAGCTGGTGTAACTCACTATGTGACAGAAGTCCTATGTCATCAGTTCAATCTGTTAGAAAGTAGAGC
AGCAGTTGCGCTTAGAGAGAATCAGGTTGAGACGGTGACCGATGTCATGCTAGAGGATGAAGATTTGCCGTTTTAG

Domains

Predicted by InterproScan.

Single-strand binding protein family (SSB)

(2-102)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbA Streptococcus mutans UA159

62.121

100

0.626
  ssbB Streptococcus sobrinus strain NIDR 6715-7

59.848

100

0.603
  ssbB/cilA Streptococcus pneumoniae TIGR4

55.303

100

0.557
  ssbB/cilA Streptococcus mitis NCTC 12261

55.303

100

0.557
  ssbB/cilA Streptococcus pneumoniae Rx1

54.545

100

0.55
  ssbB/cilA Streptococcus pneumoniae R6

54.545

100

0.55
  ssbB/cilA Streptococcus mitis SK321

54.545

100

0.55
  ssbB/cilA Streptococcus pneumoniae D39

54.545

100

0.55
  ssbB Lactococcus lactis subsp. cremoris KW2

58.974

89.313

0.527
  ssb Latilactobacillus sakei subsp. sakei 23K

46.018

86.26

0.397

Multiple sequence alignment