Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   G3M63_RS00070 Genome accession   NZ_CP048629
Coordinates   18612..19052 (-) Length   146 a.a.
NCBI ID   WP_163981512.1    Uniprot ID   A0A6C0TLK7
Organism   Pseudomonas sp. OIL-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
IScluster/Tn 16199..17763 18612..19052 flank 849


Gene organization within MGE regions


Location: 16199..19052
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G3M63_RS00060 (G3M63_00060) - 16199..17763 (+) 1565 WP_163984141.1 IS3 family transposase -
  G3M63_RS00065 (G3M63_00065) - 17708..18517 (-) 810 WP_163981511.1 hypothetical protein -
  G3M63_RS00070 (G3M63_00070) pilA 18612..19052 (-) 441 WP_163981512.1 pilin Machinery gene

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 14964.16 Da        Isoelectric Point: 6.8983

>NTDB_id=421955 G3M63_RS00070 WP_163981512.1 18612..19052(-) (pilA) [Pseudomonas sp. OIL-1]
MKAQMQKGFTLIELMIVVAIIGILAAIALPAYQDYTVRARVSEAIVAASGVKATVSENIASKNAIAAGSCLGFTDNTTAT
DNVSSVKCADETGKITVVTTAQAGAVTLSLSPALEAGTGRVTWNCGVADLANHAKYVPTECRNTAT

Nucleotide


Download         Length: 441 bp        

>NTDB_id=421955 G3M63_RS00070 WP_163981512.1 18612..19052(-) (pilA) [Pseudomonas sp. OIL-1]
ATGAAAGCTCAAATGCAGAAAGGTTTTACACTTATCGAACTGATGATCGTGGTGGCGATTATTGGTATTTTGGCGGCTAT
TGCTTTGCCGGCTTATCAGGATTATACGGTTCGGGCACGTGTGTCTGAAGCAATAGTAGCTGCTTCTGGCGTTAAGGCCA
CGGTGTCTGAGAATATTGCAAGTAAAAATGCAATAGCCGCTGGTTCTTGCTTGGGGTTCACAGACAATACAACTGCCACT
GATAATGTTTCTTCGGTGAAATGTGCTGACGAGACAGGTAAAATTACAGTAGTGACCACCGCCCAGGCTGGAGCTGTTAC
GTTGAGTCTTAGTCCTGCGTTGGAAGCGGGCACAGGGCGTGTCACTTGGAACTGTGGCGTAGCAGATCTTGCTAATCATG
CCAAATATGTTCCTACAGAGTGCCGTAACACAGCGACTTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(6-29)

Pilin (bacterial filament) (Pilin)

(37-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6C0TLK7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

46.154

100

0.534
  comP Acinetobacter baylyi ADP1

51.351

100

0.521
  pilA2 Legionella pneumophila strain ERS1305867

51.825

93.836

0.486
  pilA2 Legionella pneumophila str. Paris

51.825

93.836

0.486
  pilE Neisseria gonorrhoeae strain FA1090

40.719

100

0.466
  pilE Neisseria gonorrhoeae MS11

39.634

100

0.445
  pilA/pilA1 Eikenella corrodens VA1

39.103

100

0.418
  pilA Haemophilus influenzae 86-028NP

43.411

88.356

0.384
  pilA/pilAII Pseudomonas stutzeri DSM 10701

38.571

95.89

0.37

Multiple sequence alignment