Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GYM74_RS07425 Genome accession   NZ_CP048265
Coordinates   1689757..1690866 (-) Length   369 a.a.
NCBI ID   WP_220217593.1    Uniprot ID   -
Organism   Gilliamella sp. ESL0405     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1684757..1695866
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GYM74_RS07405 (GYM74_07380) ygfZ 1685490..1686383 (+) 894 WP_220217589.1 tRNA-modifying protein YgfZ -
  GYM74_RS07410 (GYM74_07385) - 1686518..1687669 (-) 1152 WP_220217590.1 M20 aminoacylase family protein -
  GYM74_RS07415 (GYM74_07390) - 1687806..1688585 (-) 780 WP_220217591.1 A24 family peptidase -
  GYM74_RS07420 (GYM74_07395) hofC 1688585..1689760 (-) 1176 WP_220217592.1 protein transport protein HofC -
  GYM74_RS07425 (GYM74_07400) pilB 1689757..1690866 (-) 1110 WP_220217593.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  GYM74_RS07430 (GYM74_07405) ppdD 1690897..1691328 (-) 432 WP_220217594.1 prepilin peptidase-dependent pilin -
  GYM74_RS12405 ampD 1691521..1692078 (+) 558 Protein_1453 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GYM74_RS12410 - 1692091..1692390 (+) 300 WP_370634060.1 YqcC family protein -
  GYM74_RS07440 (GYM74_07415) mltG 1692547..1693557 (-) 1011 WP_370634061.1 endolytic transglycosylase MltG -
  GYM74_RS07445 (GYM74_07420) alr 1693566..1694651 (-) 1086 WP_220217597.1 alanine racemase -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 40919.51 Da        Isoelectric Point: 5.7082

>NTDB_id=420312 GYM74_RS07425 WP_220217593.1 1689757..1690866(-) (pilB) [Gilliamella sp. ESL0405]
MQEDKLVERINDILLSALEKRASDIHFEPYQTYYRIRMRIDGVLQNKASAPLSLAKQITARLKIMSNLNIAEQRLPQDGQ
LVIDHFAMRIATLPVINGEKIVLRVMDNEQLDLSIYDLGLSPQDLITFEKILSYPQGLILVTGPTGSGKTVTLYSALKRL
NKEDCNICSVEDPIEIPLDGINQTAINPKAGLTFSVILRALLRQDPDVMMIGEIRDQQTAEIAIQASQTGHLVLSTLHTN
SSIEAITRLNQMGIENYLLASSLKLVIAQRLVRKLCIECKTAASEYILLGNEFVPHYLAIGCQHCVGGYSGRIGLYELLV
ITPEIQQQILAKQTITPTNMTSLKKAGEELVKNGITSLAEIDRVLGDSE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=420312 GYM74_RS07425 WP_220217593.1 1689757..1690866(-) (pilB) [Gilliamella sp. ESL0405]
ATGCAAGAAGATAAATTGGTTGAACGTATTAACGACATATTATTATCAGCGCTTGAAAAGCGAGCTTCAGATATTCACTT
TGAACCTTATCAAACCTATTACCGCATCCGGATGCGTATTGACGGCGTTTTACAAAACAAGGCAAGTGCACCTTTGTCTT
TAGCTAAGCAGATAACGGCAAGACTAAAAATTATGTCCAATTTAAATATAGCCGAACAGCGATTGCCGCAGGACGGTCAA
TTGGTGATTGACCATTTTGCCATGCGCATTGCCACTTTACCGGTCATCAATGGCGAAAAAATCGTATTGCGGGTTATGGA
TAACGAGCAGTTAGACTTAAGCATTTATGATTTGGGATTATCGCCGCAGGATCTAATAACTTTCGAAAAAATCCTAAGTT
ATCCGCAAGGCTTGATTTTGGTCACCGGCCCAACAGGTAGCGGTAAAACGGTCACCTTATACAGTGCACTAAAACGACTG
AATAAAGAAGATTGTAATATTTGCAGTGTCGAAGATCCGATCGAAATTCCACTCGACGGCATTAATCAAACCGCAATCAA
TCCAAAAGCAGGGTTAACTTTTTCAGTTATCTTACGTGCTTTGCTCAGACAAGACCCTGACGTGATGATGATTGGTGAAA
TTCGTGACCAGCAAACCGCTGAAATTGCCATTCAAGCATCACAAACAGGGCATTTAGTTCTATCAACCTTACACACCAAT
TCAAGCATTGAAGCTATTACTCGCCTTAATCAAATGGGCATTGAAAATTATCTGCTTGCATCAAGTTTAAAACTGGTGAT
TGCCCAACGGTTAGTGCGTAAACTTTGTATTGAATGCAAAACCGCAGCCAGTGAATACATATTATTAGGCAATGAGTTCG
TACCACATTATTTAGCCATTGGTTGCCAACATTGTGTTGGCGGGTATTCGGGGCGAATTGGTTTGTACGAATTATTAGTT
ATTACGCCGGAAATTCAACAGCAAATTTTAGCGAAGCAAACAATCACGCCAACCAATATGACTAGCTTGAAAAAAGCGGG
TGAAGAGCTGGTAAAAAATGGCATCACAAGTCTTGCTGAAATAGATCGGGTGTTAGGGGATAGCGAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

50

100

0.52
  pilB Vibrio cholerae strain A1552

49.219

100

0.512
  pilB Vibrio parahaemolyticus RIMD 2210633

48.329

100

0.509
  pilB Vibrio campbellii strain DS40M4

48.303

100

0.501
  pilB Acinetobacter baylyi ADP1

47.273

100

0.493
  pilB Haemophilus influenzae Rd KW20

48.656

100

0.491
  pilB Haemophilus influenzae 86-028NP

48.387

100

0.488
  pilB Acinetobacter baumannii D1279779

45.974

100

0.48
  pilB/pilB1 Synechocystis sp. PCC 6803

42.239

100

0.45
  pilF Neisseria gonorrhoeae MS11

42.857

100

0.447
  pilB Glaesserella parasuis strain SC1401

44.565

99.729

0.444
  pilF Thermus thermophilus HB27

42.021

100

0.428
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.365

100

0.42
  ctsE Campylobacter jejuni subsp. jejuni 81-176

40.483

100

0.409

Multiple sequence alignment