Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GYM75_RS03960 Genome accession   NZ_CP048264
Coordinates   896192..897298 (+) Length   368 a.a.
NCBI ID   WP_220216869.1    Uniprot ID   -
Organism   Gilliamella sp. ESL0441     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 891192..902298
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GYM75_RS03935 (GYM75_03935) - 891461..892222 (-) 762 WP_220216865.1 DeoR/GlpR family DNA-binding transcription regulator -
  GYM75_RS03940 (GYM75_03940) alr 892455..893543 (+) 1089 WP_220216866.1 alanine racemase -
  GYM75_RS03945 (GYM75_03945) mltG 893547..894563 (+) 1017 WP_220216867.1 endolytic transglycosylase MltG -
  GYM75_RS12360 - 894647..894970 (-) 324 WP_370632158.1 YqcC family protein -
  GYM75_RS12365 ampD 894989..895528 (-) 540 Protein_770 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GYM75_RS03955 (GYM75_03955) ppdD 895731..896159 (+) 429 WP_255556843.1 prepilin peptidase-dependent pilin -
  GYM75_RS03960 (GYM75_03960) pilB 896192..897298 (+) 1107 WP_220216869.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  GYM75_RS03965 (GYM75_03965) hofC 897295..898470 (+) 1176 WP_220216870.1 protein transport protein HofC -
  GYM75_RS03970 (GYM75_03970) - 898470..899219 (+) 750 WP_220216871.1 A24 family peptidase -
  GYM75_RS03975 (GYM75_03975) - 899425..900579 (+) 1155 WP_220216872.1 M20 aminoacylase family protein -
  GYM75_RS03980 (GYM75_03980) ygfZ 900758..901636 (-) 879 WP_220216873.1 tRNA-modifying protein YgfZ -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41084.00 Da        Isoelectric Point: 7.8244

>NTDB_id=420298 GYM75_RS03960 WP_220216869.1 896192..897298(+) (pilB) [Gilliamella sp. ESL0441]
MQEQQLVECIDKLLLSALEKRASDIHFEPYQHSYRIRMRIDGVLQNMSSPSLTLAKKIVARLKIMAKLNIAEQRLPQDGQ
LIIKNYTMRISTIPVQNGEKVVLRVMDNYESELTLDDLGLTEDNLRTFRQILNQPQGLILVTGPTGSGKTMTLYCGLKIL
NQPSCNICSVEDPIEISVEGINQTSVNPKAGLEFATVLRSLLRQDPDVIMVGEIRDYETAEIAIQAAQTGHLVLSTLHTN
SSIEGITRLNQIGIERFLIASCLKLIIAQRLVRKLCPYCKVIANELIVMNKKSIPHYIAKGCEKCIGGYVGRVGLYEFLV
ITPDVQHQILNEQAISPLNFISLKKAGQHLVKNGITSLDELNRVLGDI

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=420298 GYM75_RS03960 WP_220216869.1 896192..897298(+) (pilB) [Gilliamella sp. ESL0441]
ATGCAAGAACAGCAATTAGTTGAATGTATTGATAAATTATTACTTTCGGCATTAGAAAAAAGAGCTTCGGATATTCATTT
TGAGCCTTATCAACATAGTTACCGTATACGTATGCGTATTGATGGGGTTTTGCAAAATATGTCATCACCTTCCCTTACGT
TAGCCAAAAAAATAGTAGCTCGTTTAAAGATTATGGCTAAATTAAATATAGCTGAACAACGTTTACCCCAAGATGGGCAA
TTGATAATTAAAAATTATACTATGCGTATTTCGACCATACCTGTCCAAAATGGTGAAAAAGTTGTTTTACGTGTAATGGA
TAATTATGAGTCTGAGCTCACGTTAGATGATTTAGGTTTAACCGAAGATAATCTACGTACTTTTAGACAGATTTTGAATC
AACCGCAAGGATTGATATTAGTCACTGGTCCTACGGGTAGTGGTAAGACCATGACGCTTTATTGTGGTTTAAAAATCTTA
AATCAACCTAGTTGTAATATCTGTAGTGTTGAAGATCCGATTGAAATTTCGGTTGAAGGAATCAATCAAACTTCGGTCAA
TCCTAAAGCTGGTTTGGAATTTGCGACGGTTTTGCGTTCGTTATTAAGGCAAGATCCAGATGTAATAATGGTTGGTGAAA
TTCGTGATTATGAAACAGCAGAAATTGCTATTCAGGCCGCCCAAACAGGACATTTAGTATTATCAACCTTACATACCAAT
TCAAGTATAGAAGGAATAACACGGCTTAATCAGATAGGAATAGAAAGATTTCTCATCGCATCATGTTTAAAACTAATTAT
TGCACAACGATTGGTACGTAAGCTTTGCCCCTATTGTAAAGTGATTGCTAATGAGTTGATTGTAATGAATAAAAAATCAA
TACCTCACTATATCGCCAAAGGATGTGAAAAATGTATTGGTGGCTATGTTGGACGAGTAGGATTATACGAATTTTTAGTG
ATTACACCTGATGTTCAACATCAAATATTAAATGAACAAGCTATCTCTCCATTAAATTTTATTTCATTAAAAAAAGCAGG
GCAACACTTAGTCAAAAACGGGATTACATCTTTAGATGAATTGAATAGAGTGTTAGGCGATATATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

49.74

100

0.519
  pilB Acinetobacter baumannii D1279779

47.938

100

0.505
  pilB Vibrio parahaemolyticus RIMD 2210633

49.465

100

0.503
  pilB Acinetobacter baylyi ADP1

47.273

100

0.495
  pilB Vibrio cholerae strain A1552

48.533

100

0.495
  pilB Vibrio campbellii strain DS40M4

48.128

100

0.489
  pilB Haemophilus influenzae 86-028NP

46.612

100

0.467
  pilB Haemophilus influenzae Rd KW20

46.612

100

0.467
  pilF Neisseria gonorrhoeae MS11

44.562

100

0.457
  pilB/pilB1 Synechocystis sp. PCC 6803

40.554

100

0.437
  ctsE Campylobacter jejuni subsp. jejuni 81-176

42.553

100

0.435
  pilB Glaesserella parasuis strain SC1401

42.896

99.457

0.427
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.157

100

0.416
  pilF Thermus thermophilus HB27

39.529

100

0.41

Multiple sequence alignment