Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   GSF71_RS02180 Genome accession   NZ_CP048116
Coordinates   445239..446129 (-) Length   296 a.a.
NCBI ID   WP_076648214.1    Uniprot ID   -
Organism   Latilactobacillus sakei strain MBEL1397     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 440239..451129
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GSF71_RS02145 - 441305..442315 (-) 1011 WP_025016388.1 class I SAM-dependent methyltransferase -
  GSF71_RS02150 - 442439..442777 (-) 339 WP_056947123.1 hypothetical protein -
  GSF71_RS02155 comGF 442740..443252 (-) 513 WP_025016386.1 competence type IV pilus minor pilin ComGF Machinery gene
  GSF71_RS02160 comGE 443227..443532 (-) 306 WP_025016385.1 hypothetical protein Machinery gene
  GSF71_RS02165 comGD 443519..443971 (-) 453 WP_076648209.1 competence type IV pilus minor pilin ComGD Machinery gene
  GSF71_RS02170 comGC 443943..444242 (-) 300 WP_076645268.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  GSF71_RS02175 comGB 444239..445246 (-) 1008 WP_076648212.1 type II secretion system F family protein Machinery gene
  GSF71_RS02180 comGA 445239..446129 (-) 891 WP_076648214.1 competence type IV pilus ATPase ComGA Machinery gene
  GSF71_RS02185 - 446244..446975 (-) 732 WP_011375002.1 YebC/PmpR family DNA-binding transcriptional regulator -
  GSF71_RS02190 - 447066..447566 (-) 501 WP_016265379.1 VanZ family protein -
  GSF71_RS02195 - 447663..448037 (+) 375 WP_076648217.1 hypothetical protein -
  GSF71_RS02205 - 448224..449624 (-) 1401 WP_025016379.1 hypothetical protein -
  GSF71_RS02210 - 449638..450693 (-) 1056 WP_162100830.1 hypothetical protein -

Sequence

Protein


Download         Length: 296 a.a.        Molecular weight: 32940.80 Da        Isoelectric Point: 6.4932

>NTDB_id=419916 GSF71_RS02180 WP_076648214.1 445239..446129(-) (comGA) [Latilactobacillus sakei strain MBEL1397]
MSTEEKLTTLLKHCAKQRVQDLYFTPTIGGWRLTERRQSDLVTNQELDKPTGTRYLNRLKYMAGMDISETRRAQTGRSEL
QLADQTIYLRLATVGDFLNRESLVIRFIYPIGAIYHCDDQQILAQLTQMSRQAGLILFAGPTGSGKTTSLYHLAQQSMSA
KMVVAIEDPIEIVAPEFLQLQVNDNAGLSYAELLKISLRLRPDTLIIGEIRDLETAQYAVSAALSGHLVLSTIHARSTQG
VVARLLDLGISEMQLRACLTGIAYQSLVTKDDVVQAHYELLTGPDYFSKKEGGCDV

Nucleotide


Download         Length: 891 bp        

>NTDB_id=419916 GSF71_RS02180 WP_076648214.1 445239..446129(-) (comGA) [Latilactobacillus sakei strain MBEL1397]
ATGTCAACTGAAGAAAAGCTAACAACTTTATTAAAGCATTGTGCTAAACAACGTGTTCAAGATCTTTATTTTACACCGAC
AATAGGTGGCTGGCGCTTGACGGAACGTCGTCAGAGCGATTTGGTCACCAATCAAGAATTGGATAAACCGACTGGAACGC
GTTATTTAAATCGACTGAAGTATATGGCGGGGATGGATATCAGCGAGACTCGGCGTGCGCAAACCGGCCGCTCGGAATTA
CAGCTAGCCGACCAAACAATTTATTTACGCCTAGCGACGGTCGGGGATTTTTTAAATCGCGAAAGCTTGGTCATTCGGTT
TATCTATCCAATTGGTGCCATCTATCATTGTGATGATCAACAGATCTTGGCGCAATTAACGCAGATGAGTCGACAGGCGG
GCTTAATTCTTTTCGCTGGGCCGACTGGTTCTGGCAAAACGACTAGTCTCTATCATCTGGCCCAACAATCAATGAGTGCC
AAGATGGTGGTTGCTATTGAAGATCCGATTGAAATTGTTGCACCTGAGTTTTTGCAACTGCAGGTCAACGATAATGCGGG
ATTGAGCTACGCCGAGCTATTAAAAATTAGTTTACGACTGCGACCGGATACGCTGATTATCGGTGAGATTCGCGATTTAG
AGACAGCCCAATACGCGGTTTCTGCGGCCTTAAGTGGCCACCTGGTATTGAGTACTATTCACGCTCGGAGTACACAGGGC
GTGGTCGCGCGCTTATTGGATCTGGGGATTTCTGAGATGCAATTGCGGGCCTGTCTAACGGGAATTGCCTACCAATCGTT
GGTGACAAAAGACGACGTTGTGCAAGCCCATTATGAATTATTAACTGGTCCAGATTATTTCTCAAAAAAAGAAGGTGGTT
GCGATGTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Latilactobacillus sakei subsp. sakei 23K

98.649

100

0.986
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

42.642

89.527

0.382
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

40.876

92.568

0.378
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

42.264

89.527

0.378
  comGA/cglA/cilD Streptococcus pneumoniae R6

42.264

89.527

0.378
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

42.264

89.527

0.378
  comGA/cglA/cilD Streptococcus pneumoniae D39

42.264

89.527

0.378
  comGA Lactococcus lactis subsp. cremoris KW2

37.458

100

0.378
  comYA Streptococcus gordonii str. Challis substr. CH1

40.59

91.554

0.372

Multiple sequence alignment