Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GSS20_RS12500 Genome accession   NZ_CP047995
Coordinates   2702002..2703687 (+) Length   561 a.a.
NCBI ID   WP_031845921.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain 20150710009     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2697002..2708687
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GSS20_RS12480 (GSS20_13120) pdhR 2698586..2699353 (-) 768 WP_005462576.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  GSS20_RS12485 (GSS20_13125) ampD 2699759..2700310 (-) 552 WP_005484832.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GSS20_RS12490 (GSS20_13130) nadC 2700403..2701290 (+) 888 WP_015297249.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GSS20_RS12495 (GSS20_13135) pilA 2701553..2701990 (+) 438 WP_021453050.1 pilin Machinery gene
  GSS20_RS12500 (GSS20_13140) pilB 2702002..2703687 (+) 1686 WP_031845921.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GSS20_RS12505 (GSS20_13145) pilC 2703712..2704935 (+) 1224 WP_005462561.1 type II secretion system F family protein Machinery gene
  GSS20_RS12510 (GSS20_13150) pilD 2705000..2705869 (+) 870 WP_025524821.1 prepilin peptidase Machinery gene
  GSS20_RS12515 (GSS20_13155) coaE 2705870..2706484 (+) 615 WP_005480887.1 dephospho-CoA kinase -
  GSS20_RS12520 (GSS20_13160) zapD 2706512..2707252 (+) 741 WP_023624252.1 cell division protein ZapD -
  GSS20_RS12525 (GSS20_13165) yacG 2707321..2707515 (+) 195 WP_005462546.1 DNA gyrase inhibitor YacG -
  GSS20_RS12530 (GSS20_13170) rplS 2707889..2708242 (-) 354 WP_005462554.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62408.26 Da        Isoelectric Point: 5.5270

>NTDB_id=419139 GSS20_RS12500 WP_031845921.1 2702002..2703687(+) (pilB) [Vibrio parahaemolyticus strain 20150710009]
MITNLSTVLRQKGLLTLSQEESLFEQIKASGISMPEALLSSGFFTSSELAEHLSSIFGLNQPELSQYEYASLCQQLGLRE
LITRHNALPLHRTPSTLLLAVADPTNQQAEDDFRFATGLQVELVLADFRELSAAIRRLYGRSLSHQKSGLKEINQEELAS
LVDVGADEIDNIEDLSQDESPVSRYINQILLDAVRKGASDIHFEPYEKMYRVRLRCDGILIETQQPPNHLSRRLSARIKI
LSKLDIAERRLPQDGRIKLKLNQDTAIDMRVSTLPTLFGEKIVLRLLDSSSASLDIDKLGYSEQQKQLYLEALRRPQGMI
LMTGPTGSGKTVSLYTGLNILNKPEINISTAEDPVEINLSGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLDT
AEIAIKASQTGHLVLSTLHTNSAAETIIRLSNMGVESFNLASSLSLIIAQRLARKLCPYCKQPQEHTVQLQHLDIQTTDN
IFKANPDGCNECTHGYSGRTGIYEVMRFDESLSEALIKGASVHELEKLAIANGMSTLQMSGIEKLKQGITSFSELQRVLY
F

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=419139 GSS20_RS12500 WP_031845921.1 2702002..2703687(+) (pilB) [Vibrio parahaemolyticus strain 20150710009]
GTGATAACCAACCTCTCCACGGTTCTTCGTCAAAAAGGGTTACTGACCTTATCCCAGGAAGAATCGTTATTTGAGCAAAT
CAAAGCTTCTGGCATCTCGATGCCGGAGGCTTTACTCAGTTCTGGATTCTTCACATCAAGCGAACTGGCCGAGCACTTAA
GTTCTATTTTTGGTTTAAACCAGCCTGAGTTATCTCAATATGAATATGCTTCGCTTTGCCAACAGCTCGGCCTACGTGAA
TTAATCACACGACATAACGCACTCCCGCTCCACCGCACGCCTTCAACGTTATTATTAGCGGTTGCCGACCCCACCAATCA
ACAAGCTGAAGATGATTTCCGCTTTGCCACTGGTTTACAGGTTGAATTGGTGTTGGCGGATTTTCGTGAACTCAGTGCAG
CAATTCGACGCTTATATGGTCGTTCATTAAGTCATCAAAAGTCTGGGTTAAAAGAAATCAACCAAGAAGAGCTAGCGAGT
CTGGTGGATGTGGGCGCAGATGAAATCGACAATATCGAAGACTTGAGCCAAGACGAATCTCCTGTTAGCCGTTACATCAA
CCAAATTCTGTTGGACGCGGTGCGTAAAGGCGCATCCGATATCCACTTTGAGCCTTATGAAAAGATGTACCGAGTTCGTC
TACGTTGCGATGGCATTTTGATCGAAACTCAGCAACCGCCAAATCACTTGAGTCGTCGCTTATCAGCTCGTATCAAAATT
CTCTCTAAACTTGATATTGCCGAGCGACGTTTACCACAAGATGGGCGAATTAAACTCAAACTGAATCAAGACACCGCGAT
TGATATGCGAGTATCCACGCTACCAACCTTGTTTGGAGAGAAAATCGTACTCCGACTGCTCGATAGCAGCTCTGCATCAC
TGGATATCGACAAGCTTGGTTATAGTGAGCAACAAAAACAGCTGTATTTAGAAGCTCTGCGTCGTCCACAAGGTATGATT
CTGATGACAGGCCCAACAGGAAGCGGCAAAACCGTTTCGTTGTACACTGGATTAAATATTCTCAATAAACCAGAGATCAA
TATATCCACAGCAGAGGATCCTGTGGAAATTAACTTATCGGGCATTAACCAAGTTCAAGTCCAACCTAAGATTGGTTTTG
GCTTTGCAGAAGCACTACGCTCTTTCCTGCGTCAAGATCCCGACGTGGTCATGGTGGGTGAGATTCGAGACCTAGATACT
GCGGAAATCGCCATCAAGGCGTCCCAAACCGGTCACTTAGTGCTCTCTACTTTGCATACCAACTCTGCAGCTGAAACCAT
CATTCGCTTATCAAACATGGGCGTGGAAAGTTTTAACCTCGCCTCTTCGCTTAGTTTGATTATTGCCCAACGCCTAGCTC
GCAAACTTTGTCCATATTGTAAACAACCGCAAGAGCATACCGTTCAACTTCAGCACCTCGACATCCAAACAACTGACAAC
ATTTTTAAAGCAAACCCAGATGGTTGCAACGAATGTACCCATGGTTATTCTGGCCGAACAGGTATCTATGAAGTCATGCG
CTTTGATGAATCTCTATCCGAAGCTCTTATTAAAGGAGCCTCGGTACATGAACTGGAAAAGCTTGCTATTGCGAACGGCA
TGAGCACCTTGCAAATGTCCGGAATAGAAAAACTCAAACAAGGCATCACAAGCTTTAGTGAGTTACAACGCGTGCTCTAT
TTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-144)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio parahaemolyticus RIMD 2210633

98.039

100

0.98
  pilB Vibrio campbellii strain DS40M4

90.018

100

0.9
  pilB Vibrio cholerae strain A1552

73.665

100

0.738
  pilB Acinetobacter baumannii D1279779

50.746

95.544

0.485
  pilB Legionella pneumophila strain ERS1305867

48.799

96.435

0.471
  pilB Acinetobacter baylyi ADP1

50.484

92.157

0.465
  pilF Neisseria gonorrhoeae MS11

44.681

100

0.449
  pilB/pilB1 Synechocystis sp. PCC 6803

37.099

100

0.392
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.491

100

0.371
  pilF Thermus thermophilus HB27

36.637

100

0.369

Multiple sequence alignment