Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   GSS61_RS12745 Genome accession   NZ_CP047985
Coordinates   2695005..2696753 (-) Length   582 a.a.
NCBI ID   WP_025623689.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain HZ-52     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2690005..2701753
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GSS61_RS12725 (GSS61_13310) - 2690687..2691526 (-) 840 WP_005486862.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  GSS61_RS12730 (GSS61_13315) - 2691596..2693113 (-) 1518 WP_062852346.1 SPOR domain-containing protein -
  GSS61_RS12735 (GSS61_13320) aroB 2693166..2694266 (-) 1101 WP_062852347.1 3-dehydroquinate synthase -
  GSS61_RS12740 (GSS61_13325) aroK 2694298..2694816 (-) 519 WP_005381319.1 shikimate kinase AroK -
  GSS61_RS12745 (GSS61_13330) pilQ 2695005..2696753 (-) 1749 WP_025623689.1 type IV pilus secretin PilQ Machinery gene
  GSS61_RS12750 (GSS61_13335) pilP 2696785..2697300 (-) 516 WP_005480477.1 pilus assembly protein PilP Machinery gene
  GSS61_RS12755 (GSS61_13340) pilO 2697290..2697883 (-) 594 WP_005480482.1 type 4a pilus biogenesis protein PilO Machinery gene
  GSS61_RS12760 (GSS61_13345) pilN 2697876..2698454 (-) 579 WP_015297396.1 PilN domain-containing protein Machinery gene
  GSS61_RS12765 (GSS61_13350) pilM 2698438..2699454 (-) 1017 WP_005480475.1 type IV pilus biogenesis protein PilM Machinery gene

Sequence

Protein


Download         Length: 582 a.a.        Molecular weight: 63755.88 Da        Isoelectric Point: 4.4619

>NTDB_id=419057 GSS61_RS12745 WP_025623689.1 2695005..2696753(-) (pilQ) [Vibrio parahaemolyticus strain HZ-52]
MKMRQGLTRTPRMLTGLFSLWLFLLLAPLAAAEDVQSNALKSIDFRTNKSKDAVIVVELASPAAIVDVKRVQEGLSIDLM
NTSVKDEQLYLLDVKDFATVVESVEVFRDTSTTRLVAYIDEEYTHDYRLTGRYLEITVSKLKPNEKVPDKSILEKEGKLI
SINFQDIPVRNVLQLIADYNQFNLVVSDSVEGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPTDELDLREKQQ
LEKQKLTEEMGELSSEIIKVNFAKASDIAEMINGEGNISMLSERGSMTIDERTNSLLIRELPENIAVIREIIESLDIPVK
QVQIEARIVTISEGNMDELGIRWGFTSINGNNTVGGSIENNLATIGLYEGGSDGEGEEGGDGVGIDDFLNVNLAATNKSA
ASIAFQVAKLGSDTLLDLELSALQQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATSVAFKKAVLSLKVTPQ
ITPDNRLVLDLSVTQDRPGKVVKTGTGEAMAIDTQRIGTQVLVNNGETVVLGGIFQHSITNSVDKVPLLGDLPLLGALFR
RSYENVGKSELLIFVTPKVVIQ

Nucleotide


Download         Length: 1749 bp        

>NTDB_id=419057 GSS61_RS12745 WP_025623689.1 2695005..2696753(-) (pilQ) [Vibrio parahaemolyticus strain HZ-52]
ATGAAAATGAGACAAGGATTAACACGTACACCAAGGATGCTTACTGGGCTCTTCTCCTTATGGTTGTTTTTACTTCTGGC
CCCTTTAGCAGCAGCAGAAGATGTACAGTCTAATGCGCTCAAGAGCATCGACTTTCGCACTAACAAAAGCAAAGATGCCG
TGATTGTGGTGGAGCTTGCATCCCCGGCGGCTATTGTTGATGTTAAACGTGTTCAAGAAGGATTAAGCATTGATTTAATG
AACACGTCGGTAAAAGATGAACAGCTTTACCTTCTCGACGTGAAAGATTTCGCGACGGTGGTGGAAAGTGTGGAAGTGTT
CCGCGATACCTCCACCACACGATTAGTTGCGTATATCGATGAAGAATACACGCATGACTATCGTTTGACGGGGCGTTATC
TTGAAATCACTGTCAGCAAGCTTAAACCAAATGAAAAAGTGCCAGACAAGAGCATCTTAGAAAAAGAAGGCAAGCTGATC
TCGATTAACTTCCAAGACATTCCTGTGCGTAACGTTTTGCAGCTGATTGCAGATTACAACCAGTTTAACTTGGTGGTTTC
GGATTCAGTCGAAGGCAACCTTACACTGCGTCTTGATGGTGTGCCGTGGCAACAAGTGCTCGACATCATCTTGCAAGTGA
AAGGGTTAGATAAGCGTGTTGATGGCAACGTGATTTTGGTCGCTCCAACCGATGAGCTGGATTTGCGCGAGAAGCAGCAG
TTAGAAAAACAAAAACTGACGGAAGAAATGGGTGAGCTTTCATCAGAAATCATCAAAGTCAATTTTGCTAAAGCTTCGGA
TATTGCAGAGATGATCAATGGTGAAGGCAACATTAGCATGTTGTCAGAGCGTGGCAGTATGACCATTGATGAGCGAACCA
ATTCGCTATTAATCAGAGAATTGCCAGAGAACATCGCCGTTATTCGCGAAATCATTGAATCGCTCGATATCCCAGTCAAA
CAAGTACAAATTGAAGCCCGAATTGTGACCATCAGTGAAGGTAACATGGACGAGTTAGGTATTCGTTGGGGCTTCACATC
TATTAATGGTAATAACACCGTTGGTGGCTCTATTGAGAACAACCTTGCGACAATCGGCTTGTACGAAGGTGGTAGTGACG
GCGAAGGTGAAGAGGGTGGTGATGGTGTTGGTATCGATGATTTCCTTAATGTGAACTTAGCTGCAACTAATAAAAGTGCT
GCAAGCATTGCGTTCCAGGTGGCAAAGTTAGGTTCTGATACATTATTGGACTTAGAGCTGTCTGCATTACAGCAAGAATC
AAAAGCAGAAATTATTTCGAGCCCACGATTGATCACGACCAATAAGAAGCCTGCGTACATTGAACAAGGTACTGAAATTC
CTTATCTAGAATCTTCATCGAGTGGTGCAACGTCAGTGGCCTTTAAAAAAGCGGTGTTGAGCTTGAAGGTGACACCGCAG
ATCACACCTGACAATCGTTTAGTGTTGGATCTGAGTGTGACCCAAGACCGACCTGGTAAGGTAGTGAAAACGGGCACAGG
AGAGGCAATGGCAATTGATACGCAGCGCATTGGCACCCAAGTTCTGGTTAATAATGGTGAGACGGTTGTGCTCGGTGGTA
TTTTCCAGCACAGTATTACTAACTCAGTGGATAAAGTGCCATTGTTGGGCGATCTTCCGCTATTAGGCGCACTTTTCCGC
CGAAGTTACGAAAACGTTGGTAAAAGTGAGTTGCTAATTTTCGTTACGCCTAAGGTCGTTATTCAGTAG

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(256-321)

Bacterial type II and III secretion system protein (Secretin)

(422-580)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

91.237

100

0.912
  pilQ Vibrio cholerae strain A1552

76.408

97.595

0.746
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

76.408

97.595

0.746
  pilQ Pseudomonas aeruginosa PAK

38.71

100

0.392
  pilQ Legionella pneumophila strain ERS1305867

41.273

94.502

0.39
  pilQ Legionella pneumophila strain Lp02

41.273

94.502

0.39
  pilQ Acinetobacter baumannii D1279779

37.625

100

0.387
  pilQ Acinetobacter baumannii strain A118

37.625

100

0.387
  comQ Acinetobacter baylyi ADP1

38.25

100

0.383

Multiple sequence alignment