Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   GSS61_RS12765 Genome accession   NZ_CP047985
Coordinates   2698438..2699454 (-) Length   338 a.a.
NCBI ID   WP_005480475.1    Uniprot ID   A0A0L7V0I6
Organism   Vibrio parahaemolyticus strain HZ-52     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2693438..2704454
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GSS61_RS12740 (GSS61_13325) aroK 2694298..2694816 (-) 519 WP_005381319.1 shikimate kinase AroK -
  GSS61_RS12745 (GSS61_13330) pilQ 2695005..2696753 (-) 1749 WP_025623689.1 type IV pilus secretin PilQ Machinery gene
  GSS61_RS12750 (GSS61_13335) pilP 2696785..2697300 (-) 516 WP_005480477.1 pilus assembly protein PilP Machinery gene
  GSS61_RS12755 (GSS61_13340) pilO 2697290..2697883 (-) 594 WP_005480482.1 type 4a pilus biogenesis protein PilO Machinery gene
  GSS61_RS12760 (GSS61_13345) pilN 2697876..2698454 (-) 579 WP_015297396.1 PilN domain-containing protein Machinery gene
  GSS61_RS12765 (GSS61_13350) pilM 2698438..2699454 (-) 1017 WP_005480475.1 type IV pilus biogenesis protein PilM Machinery gene
  GSS61_RS12770 (GSS61_13355) - 2699597..2702167 (+) 2571 WP_005496567.1 penicillin-binding protein 1A -
  GSS61_RS12775 (GSS61_13360) oxyR 2702223..2703131 (-) 909 WP_005465160.1 DNA-binding transcriptional regulator OxyR -
  GSS61_RS12780 (GSS61_13365) - 2703302..2704030 (+) 729 WP_062852348.1 redoxin family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37776.31 Da        Isoelectric Point: 6.8323

>NTDB_id=419061 GSS61_RS12765 WP_005480475.1 2698438..2699454(-) (pilM) [Vibrio parahaemolyticus strain HZ-52]
MDKLIVTGIDIGHNSLKAVVLKPIGDQYALLGYKEILLKEGIVAENNTINHQEIVKTLKQMKKELPFGAKRVAISVPDNS
VISKKLQIEQSLDESEIEFAVVQAFSHQSPFPVEELSLDFVRLRAEEGMRGTDSYQVFATRKDVVESRVEALQQSGLKPV
LVDVHSQSLGHIWKLAAERFPEKNKYCLLDIGSLASSFTMFTEQGELFHKEFACGTRISMGTSQEDLLSDDAQAKTEQFN
RQVVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSHQLALECELLNPLGLFEMKVSKRKRRAADWQHFSTAA
GLAVRGIHWLGGVRALSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=419061 GSS61_RS12765 WP_005480475.1 2698438..2699454(-) (pilM) [Vibrio parahaemolyticus strain HZ-52]
ATGGATAAGCTAATCGTTACCGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCATTGGCGATCA
ATACGCCTTACTGGGTTACAAAGAAATATTACTTAAGGAAGGTATTGTCGCTGAAAATAACACTATAAATCATCAAGAAA
TTGTAAAGACCCTAAAGCAGATGAAAAAAGAGCTGCCTTTTGGGGCCAAGCGAGTGGCAATTTCTGTTCCAGACAACTCA
GTTATTAGCAAGAAACTGCAAATCGAACAAAGCCTTGATGAGAGCGAAATCGAGTTTGCGGTGGTACAAGCTTTTTCTCA
TCAATCTCCTTTTCCTGTGGAAGAACTGAGTTTGGATTTTGTACGCCTTCGTGCGGAAGAAGGGATGCGTGGAACGGACA
GTTATCAAGTATTCGCTACGCGCAAAGATGTGGTTGAAAGCCGAGTTGAGGCGTTACAACAGTCTGGTTTAAAGCCTGTG
TTGGTTGACGTGCACTCTCAAAGCCTAGGTCATATCTGGAAGCTTGCCGCAGAGCGTTTTCCTGAAAAAAACAAATACTG
TTTGTTAGACATCGGCTCGTTAGCGAGTTCATTCACCATGTTTACCGAGCAAGGCGAACTGTTTCATAAGGAGTTTGCGT
GCGGTACTCGTATTTCAATGGGGACGTCACAAGAAGACTTACTTTCTGATGATGCTCAAGCCAAAACGGAACAGTTCAAT
CGTCAAGTGGTTGAGCGCGTAAAACGTCAAATGCAGCTCTATACGTCGATCAACGGTTCGCAAAACATCAAAGGCATTTG
GTTATCGGGCGAGGGGGCATCGACGCCAATGTTGGCAGAAGAGCTCTCGCATCAGTTGGCATTGGAGTGCGAGTTGCTAA
ACCCACTCGGACTGTTTGAAATGAAAGTGTCGAAACGCAAGCGACGAGCGGCTGATTGGCAACATTTTTCGACAGCTGCA
GGGCTGGCGGTCCGTGGCATTCATTGGTTAGGAGGTGTGCGTGCTTTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0L7V0I6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

84.911

100

0.849
  pilM Vibrio cholerae strain A1552

48.368

99.704

0.482

Multiple sequence alignment