Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   M594_RS00895 Genome accession   NZ_CP047883
Coordinates   180312..181328 (+) Length   338 a.a.
NCBI ID   WP_153209728.1    Uniprot ID   A0A6I1UJQ4
Organism   Streptococcus mitis strain S022-V3-A4     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 175312..186328
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M594_RS00875 (M594_00895) - 175681..177498 (+) 1818 WP_173875724.1 acyltransferase family protein -
  M594_RS00880 (M594_00900) nagA 177639..178790 (+) 1152 WP_033687416.1 N-acetylglucosamine-6-phosphate deacetylase -
  M594_RS00885 (M594_00905) - 178982..179347 (+) 366 WP_173875725.1 DUF1033 family protein -
  M594_RS00890 (M594_00910) comGA/cglA/cilD 179423..180364 (+) 942 WP_173875726.1 competence type IV pilus ATPase ComGA Machinery gene
  M594_RS00895 (M594_00915) comGB/cglB 180312..181328 (+) 1017 WP_153209728.1 competence type IV pilus assembly protein ComGB Machinery gene
  M594_RS00900 (M594_00920) comGC/cglC 181330..181653 (+) 324 WP_000738641.1 comG operon protein ComGC Machinery gene
  M594_RS00905 (M594_00925) comGD/cglD 181616..181996 (+) 381 WP_173875727.1 competence type IV pilus minor pilin ComGD Machinery gene
  M594_RS00910 (M594_00930) comGE/cglE 182013..182315 (+) 303 WP_153209718.1 competence type IV pilus minor pilin ComGE Machinery gene
  M594_RS00915 (M594_00935) comGF/cglF 182278..182739 (+) 462 WP_173875728.1 competence type IV pilus minor pilin ComGF Machinery gene
  M594_RS00920 (M594_00940) comGG/cglG 182717..183130 (+) 414 WP_173875729.1 competence type IV pilus minor pilin ComGG Machinery gene
  M594_RS00925 (M594_00945) - 183163..183750 (+) 588 WP_173875730.1 class I SAM-dependent methyltransferase -
  M594_RS00930 (M594_00950) comYH 183811..184764 (+) 954 WP_173875731.1 class I SAM-dependent methyltransferase Machinery gene
  M594_RS00935 (M594_00955) - 184814..186004 (+) 1191 WP_084886490.1 acetate kinase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38595.58 Da        Isoelectric Point: 9.5998

>NTDB_id=418225 M594_RS00895 WP_153209728.1 180312..181328(+) (comGB/cglB) [Streptococcus mitis strain S022-V3-A4]
MDISQVFRLKRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSALLDKQCVTQMRTGLSQGKSFSEMMESLGFSSAI
VTQLSLAEVHGNLHLSLGKIEEYLDNLTKVKKKLIEVATYPLILLGFLFLIMLGLRNYLLPQLDSSNIATQIIGNLPQIF
LGMVGFVSILALLALTFYKRSSKMRVFSILARLPFFGIFVQTYLTAYYAREWGNMISQGMELTQIFQMMQEQGSQLFKEI
GQDLAQALQNGREFSQTIATYPFFKKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=418225 M594_RS00895 WP_153209728.1 180312..181328(+) (comGB/cglB) [Streptococcus mitis strain S022-V3-A4]
ATGGACATATCACAAGTCTTCAGGCTGAAACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCTTGTTTAA
CAATCTCTTTTCCAGCGGTTTTCATCTAGTGGAGACTATCTCATTTTTAGATAGGAGTGCTTTGTTGGACAAGCAGTGTG
TGACCCAGATGCGTACAGGTTTGTCTCAGGGGAAATCATTCTCAGAAATGATGGAAAGTTTGGGTTTTTCAAGTGCCATT
GTCACTCAGTTATCCCTAGCTGAAGTTCATGGGAATCTCCACCTTAGTTTAGGAAAGATAGAAGAGTATCTGGACAATCT
GACCAAGGTCAAGAAAAAATTAATTGAAGTAGCGACCTATCCCTTGATTTTGCTGGGTTTTCTTTTCTTAATTATGCTGG
GGCTACGGAACTATCTACTACCACAACTGGATAGTAGCAATATTGCCACCCAAATCATTGGAAATCTGCCACAAATCTTT
CTAGGAATGGTGGGGTTTGTTTCCATACTTGCCCTTTTAGCACTAACTTTCTATAAAAGAAGTTCCAAAATGCGTGTCTT
TTCTATCTTAGCGCGTCTTCCCTTCTTTGGAATCTTTGTTCAGACTTATTTGACAGCTTATTATGCGCGTGAATGGGGGA
ATATGATTTCTCAAGGGATGGAGTTGACGCAGATTTTTCAAATGATGCAGGAACAAGGTTCCCAACTCTTTAAAGAAATC
GGTCAAGATCTAGCTCAAGCCCTACAAAATGGTCGCGAATTTTCTCAAACCATAGCAACCTATCCTTTCTTTAAGAAAGA
GTTGAGTCTTATTATCGAGTATGGTGAAGTCAAGTCCAAGCTGGGGAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTTAACCGCACCATGAATTTGGTGCAGCCACTGGTTTTTATCTTTGTGGCACTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I1UJQ4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus mitis SK321

96.746

100

0.967
  comGB/cglB Streptococcus mitis NCTC 12261

95.858

100

0.959
  comGB/cglB Streptococcus pneumoniae Rx1

95.858

100

0.959
  comGB/cglB Streptococcus pneumoniae D39

95.858

100

0.959
  comGB/cglB Streptococcus pneumoniae R6

95.858

100

0.959
  comGB/cglB Streptococcus pneumoniae TIGR4

95.858

100

0.959
  comYB Streptococcus gordonii str. Challis substr. CH1

72.917

99.408

0.725
  comYB Streptococcus mutans UA140

58.228

93.491

0.544
  comYB Streptococcus mutans UA159

58.228

93.491

0.544
  comGB Lactococcus lactis subsp. cremoris KW2

50.898

98.817

0.503

Multiple sequence alignment