Detailed information NTG0418229

Bioinformatically predicted

Overview

• Name: comGF/cglF
• Type: Machinery gene
• Locus tag: M594_RS00915
• Genome accession: NZ_CP047883
• Coordinates: 182278..182739 (+)
• Length: 153 a.a.
• NCBI ID: WP_173875728.1
• Uniprot ID: A0A6M9F8C1
• Organism: Streptococcus mitis strain S022-V3-A4
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 177278..187739

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M594_RS00880 (M594_00900)	nagA	177639..178790 (+)	1152	WP_033687416.1	N-acetylglucosamine-6-phosphate deacetylase	-
M594_RS00885 (M594_00905)	-	178982..179347 (+)	366	WP_173875725.1	DUF1033 family protein	-
M594_RS00890 (M594_00910)	comGA/cglA/cilD	179423..180364 (+)	942	WP_173875726.1	competence type IV pilus ATPase ComGA	Machinery gene
M594_RS00895 (M594_00915)	comGB/cglB	180312..181328 (+)	1017	WP_153209728.1	competence type IV pilus assembly protein ComGB	Machinery gene
M594_RS00900 (M594_00920)	comGC/cglC	181330..181653 (+)	324	WP_000738641.1	comG operon protein ComGC	Machinery gene
M594_RS00905 (M594_00925)	comGD/cglD	181616..181996 (+)	381	WP_173875727.1	competence type IV pilus minor pilin ComGD	Machinery gene
M594_RS00910 (M594_00930)	comGE/cglE	182013..182315 (+)	303	WP_153209718.1	competence type IV pilus minor pilin ComGE	Machinery gene
M594_RS00915 (M594_00935)	comGF/cglF	182278..182739 (+)	462	WP_173875728.1	competence type IV pilus minor pilin ComGF	Machinery gene
M594_RS00920 (M594_00940)	comGG/cglG	182717..183130 (+)	414	WP_173875729.1	competence type IV pilus minor pilin ComGG	Machinery gene
M594_RS00925 (M594_00945)	-	183163..183750 (+)	588	WP_173875730.1	class I SAM-dependent methyltransferase	-
M594_RS00930 (M594_00950)	comYH	183811..184764 (+)	954	WP_173875731.1	class I SAM-dependent methyltransferase	Machinery gene
M594_RS00935 (M594_00955)	-	184814..186004 (+)	1191	WP_084886490.1	acetate kinase	-
M594_RS00940 (M594_00960)	rnpA	186154..186525 (+)	372	WP_000739245.1	ribonuclease P protein component	-
M594_RS00945 (M594_00965)	-	186500..187324 (+)	825	WP_217423022.1	membrane protein insertase YidC	-

Sequence

Protein

• Length: 153 a.a.
• Molecular weight: 17888.50 Da
• Isoelectric Point: 8.8053

>NTDB_id=418229 M594_RS00915 WP_173875728.1 182278..182739(+) (comGF/cglF) [Streptococcus mitis strain S022-V3-A4]
MAQNSCWLSKSHKVKAFTLLESLIALVVISGSLLLFQAMSQLLISEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVEGN
RLYMKQDGKDISIGKSKSDDFRKTDASGRGYQPMVYGLKFAQITEDNQLVRFRFQFQKGLEREFIYRVEKEKS

Nucleotide

• Length: 462 bp

>NTDB_id=418229 M594_RS00915 WP_173875728.1 182278..182739(+) (comGF/cglF) [Streptococcus mitis strain S022-V3-A4]
ATGGCTCAGAACAGTTGTTGGCTATCAAAGAGCCATAAGGTCAAGGCTTTTACCTTGTTAGAATCCCTTATTGCTCTCGT
TGTTATCAGTGGAAGCTTACTCCTCTTTCAAGCCATGAGTCAGCTCCTCATTTCAGAAGTTCGCTACCAGCAGCAAAGCG
AGCAAAAGGAGTGGCTCCTGTTTGTGGACCAGTTGGAGGCAGAATTAGATCGTTCGCAGTTTGAAAAAGTGGAAGGCAAT
CGTCTCTATATGAAGCAGGATGGCAAGGATATTTCTATAGGTAAGTCTAAATCAGATGATTTTCGGAAAACCGATGCCAG
TGGACGAGGTTATCAGCCTATGGTTTATGGCCTCAAATTTGCGCAGATTACAGAGGACAATCAATTGGTTCGCTTTCGTT
TTCAATTCCAAAAAGGCTTAGAAAGGGAGTTCATATATCGTGTGGAAAAAGAAAAAAGTTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (45-141)

3D structure

• AlphaFold DB: A0A6M9F8C1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF/cglF	Streptococcus mitis SK321	95.425	100	0.954
comGF/cglF	Streptococcus mitis NCTC 12261	92.81	100	0.928
comGF/cglF	Streptococcus pneumoniae Rx1	91.503	100	0.915
comGF/cglF	Streptococcus pneumoniae D39	91.503	100	0.915
comGF/cglF	Streptococcus pneumoniae R6	91.503	100	0.915
comGF/cglF	Streptococcus pneumoniae TIGR4	91.503	100	0.915
comGF	Lactococcus lactis subsp. cremoris KW2	40	91.503	0.366