Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   PPS_RS25410 Genome accession   NC_015733
Coordinates   5596475..5597743 (-) Length   422 a.a.
NCBI ID   WP_080563829.1    Uniprot ID   -
Organism   Pseudomonas putida S16     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5591475..5602743
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PPS_RS25395 (PPS_4922) - 5593132..5594742 (-) 1611 WP_013974601.1 AAA family ATPase -
  PPS_RS25400 (PPS_4923) aroB 5594758..5595855 (-) 1098 WP_013974602.1 3-dehydroquinate synthase -
  PPS_RS25405 (PPS_4924) aroK 5595952..5596470 (-) 519 WP_015272136.1 shikimate kinase AroK -
  PPS_RS25410 (PPS_4925) pilQ 5596475..5597743 (-) 1269 WP_080563829.1 secretin N-terminal domain-containing protein Machinery gene
  PPS_RS25415 (PPS_4926) - 5597667..5598668 (-) 1002 WP_031325416.1 pilus assembly protein PilP -
  PPS_RS25420 (PPS_4927) - 5598680..5599216 (-) 537 WP_023662590.1 PilN domain-containing protein -
  PPS_RS25425 (PPS_4928) pilM 5599213..5600094 (-) 882 WP_024087852.1 pilus assembly protein PilM -

Sequence

Protein


Download         Length: 422 a.a.        Molecular weight: 45985.80 Da        Isoelectric Point: 4.9992

>NTDB_id=41679 PPS_RS25410 WP_080563829.1 5596475..5597743(-) (pilQ) [Pseudomonas putida S16]
MKRLMKWMLAALLPFPLASAMPYQGEPLSLNFQDVEVRSVLQVLADYAGVNLVASDAVQGSVTLRLEDVPWDQALDLVLR
TKGLARRQEGNVLLVAPAAEFAAQSVDAHVGQALDAQLQPLRRELLPIHHAKAADLAELLLASLADDGILTSRGSLSVDE
RTNTLVVHQPADRLAELRQLVAQLDVPVRQVAIEARIVEANVDYEKSLGVRWGGPLYVESPRPGKDLFVDLGVERVGGSI
GLGLLRGDVLLDLELSAMEKSGNGEIISQPKVVTADKETARILKGTEVPYQETSKSGATSVAFREASLSLEVTPQITPDN
KVIMTVRVTKDEPDYVNALNNVPPIRKNEVNAKVRVTDGETIVIGGVYSTSQNNVVDKVPFFGDLPYVGRLFRRDALQEK
KSELLVFLTPRIMSDQAIAVSR

Nucleotide


Download         Length: 1269 bp        

>NTDB_id=41679 PPS_RS25410 WP_080563829.1 5596475..5597743(-) (pilQ) [Pseudomonas putida S16]
ATGAAAAGATTGATGAAATGGATGCTGGCAGCCCTGCTGCCGTTCCCGCTGGCGTCGGCAATGCCTTATCAGGGTGAACC
ACTGTCGCTGAACTTCCAGGATGTGGAAGTGCGCTCGGTGCTGCAGGTGCTGGCTGACTATGCCGGTGTCAACCTGGTTG
CCAGCGATGCCGTGCAGGGTAGCGTCACTTTGCGCCTTGAGGATGTTCCATGGGACCAGGCGCTGGATCTGGTGCTGCGC
ACCAAGGGCTTGGCCCGGCGACAGGAGGGCAATGTGCTGCTGGTGGCGCCCGCAGCGGAGTTTGCCGCGCAGTCTGTCGA
TGCCCATGTGGGCCAGGCGCTGGATGCACAGTTGCAGCCATTGCGTCGGGAGCTGTTGCCCATTCATCACGCCAAGGCCG
CCGATCTGGCAGAGCTGCTGCTGGCGAGCCTGGCGGATGACGGCATCCTCACCAGTCGGGGCAGCCTCAGTGTCGATGAG
CGCACCAATACGCTGGTGGTGCACCAGCCGGCAGACCGCCTTGCCGAGCTGAGGCAACTGGTGGCGCAGCTGGATGTGCC
GGTGCGTCAGGTGGCGATCGAGGCACGTATCGTCGAGGCCAACGTCGATTACGAGAAAAGCCTGGGGGTGCGCTGGGGTG
GGCCGCTGTATGTCGAGAGCCCGCGCCCGGGCAAGGATCTGTTCGTCGATCTCGGGGTGGAGCGGGTGGGCGGTAGCATC
GGTCTGGGCCTGTTGCGTGGCGATGTGCTGCTCGACCTGGAACTCAGTGCCATGGAAAAGAGCGGCAACGGCGAAATCAT
CTCGCAGCCCAAGGTGGTCACGGCCGACAAGGAAACGGCCAGGATCCTCAAAGGCACCGAGGTGCCGTACCAGGAAACCA
GCAAGAGCGGCGCCACCTCGGTAGCATTCCGCGAAGCCTCGTTGTCGCTGGAAGTGACCCCGCAGATCACTCCGGACAAC
AAGGTGATCATGACCGTCAGGGTGACCAAGGACGAGCCGGATTACGTCAATGCCCTGAACAACGTACCGCCGATCCGCAA
GAACGAGGTCAATGCCAAGGTTCGCGTGACGGACGGAGAAACGATCGTGATCGGTGGCGTGTACTCGACTTCGCAAAACA
ATGTGGTCGACAAGGTGCCATTTTTCGGCGATCTGCCGTATGTTGGGCGGCTGTTTCGACGCGATGCATTACAAGAGAAA
AAATCCGAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAGTGACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(51-97)

Bacterial type II/III secretion system short domain (Secretin_N)

(125-190)

Bacterial type II and III secretion system protein (Secretin)

(257-413)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

58.864

100

0.614
  comQ Acinetobacter baylyi ADP1

42.699

100

0.457
  pilQ Acinetobacter baumannii strain A118

41.83

100

0.455
  pilQ Acinetobacter baumannii D1279779

41.83

100

0.455
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

42.755

99.763

0.427
  pilQ Vibrio cholerae strain A1552

42.755

99.763

0.427
  pilQ Legionella pneumophila strain Lp02

42.823

99.052

0.424
  pilQ Legionella pneumophila strain ERS1305867

42.823

99.052

0.424
  pilQ Vibrio campbellii strain DS40M4

40.972

100

0.419
  pilQ Neisseria gonorrhoeae strain FA1090

36.364

99.052

0.36

Multiple sequence alignment