Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   FX988_RS17760 Genome accession   NZ_CP047656
Coordinates   4180627..4181832 (+) Length   401 a.a.
NCBI ID   WP_160181425.1    Uniprot ID   A0A857JMP1
Organism   Paraglaciecola mesophila strain GPM4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4175627..4186832
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FX988_RS17730 (FX988_03580) - 4175987..4176742 (-) 756 WP_160181420.1 GntR family transcriptional regulator -
  FX988_RS17735 (FX988_03581) ampE 4176958..4177770 (-) 813 WP_160181421.1 beta-lactamase regulator AmpE -
  FX988_RS17740 (FX988_03582) ampD 4177809..4178396 (-) 588 WP_160181422.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FX988_RS17745 (FX988_03583) - 4178463..4178999 (+) 537 WP_254700656.1 TIGR02281 family clan AA aspartic protease -
  FX988_RS17750 (FX988_03584) nadC 4179067..4179909 (+) 843 WP_160181423.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FX988_RS17755 (FX988_03585) - 4180115..4180561 (+) 447 WP_160181424.1 pilin -
  FX988_RS17760 (FX988_03586) pilC 4180627..4181832 (+) 1206 WP_160181425.1 type II secretion system F family protein Machinery gene
  FX988_RS17765 (FX988_03587) pilD 4181938..4182834 (+) 897 WP_160181426.1 prepilin peptidase Machinery gene
  FX988_RS17770 (FX988_03588) coaE 4182831..4183445 (+) 615 WP_160181427.1 dephospho-CoA kinase -
  FX988_RS17775 (FX988_03589) zapD 4183580..4184332 (+) 753 WP_160181428.1 cell division protein ZapD -
  FX988_RS17780 (FX988_03590) yacG 4184367..4184597 (+) 231 WP_160181429.1 DNA gyrase inhibitor YacG -
  FX988_RS17785 (FX988_03591) - 4184750..4185493 (+) 744 WP_160181430.1 glutathione peroxidase -
  FX988_RS17790 (FX988_03592) - 4185633..4186670 (+) 1038 WP_412761911.1 alpha/beta hydrolase -

Sequence

Protein


Download         Length: 401 a.a.        Molecular weight: 43138.83 Da        Isoelectric Point: 9.8848

>NTDB_id=415054 FX988_RS17760 WP_160181425.1 4180627..4181832(+) (pilC) [Paraglaciecola mesophila strain GPM4]
MPKATTTFVWSGANRKGQTVKGEISAVSVVEAKNLLRRQGVSAKKVKKLAKPFFGGQKIKPVDISIISRQIATMLSAGVT
LIQTIDMIATGHNNPNMRKMLGEIGGEVKAGNPFSTALRKHPDQFDDLYCDLVSTGEQSGALETIYDRIATYKEKAEALK
SKIKKAMFYPVAVIVVAFIVTTILLIFVVPQFEEIFSSFGAELPAFTQFVLTISRFLQEFGLFVAAGLVVAGILFSKTYK
KSKRLRDSLDSKMLKIPVIGEILKKASVARFSRTLATTFAAGVPLIGALDSAAGASGNAVFRDAILFVKKEVAGGTQMNT
AMRATAVFPDMVTQMVAIGEEAGAVDDMLSKIATIYEAEVDDMVDGLTSLLEPMIMAVLGVVIGGLIVAMYLPIFEMGNV
V

Nucleotide


Download         Length: 1206 bp        

>NTDB_id=415054 FX988_RS17760 WP_160181425.1 4180627..4181832(+) (pilC) [Paraglaciecola mesophila strain GPM4]
ATGCCTAAAGCCACCACTACCTTTGTTTGGAGCGGAGCGAACCGTAAAGGTCAAACTGTAAAAGGCGAGATCAGTGCGGT
GTCGGTTGTAGAGGCAAAAAACCTACTGCGCCGACAGGGTGTTTCGGCCAAAAAAGTGAAAAAGCTGGCAAAGCCTTTCT
TCGGTGGGCAAAAAATCAAGCCAGTGGACATCAGTATTATTTCTCGTCAGATTGCCACTATGCTAAGCGCCGGTGTGACC
TTGATTCAAACCATAGACATGATTGCCACAGGTCACAACAACCCGAATATGCGTAAAATGCTGGGTGAAATTGGGGGGGA
AGTAAAAGCCGGTAATCCCTTTTCAACCGCGCTGCGCAAACATCCAGATCAATTTGACGATTTATACTGTGATTTGGTAT
CCACCGGGGAGCAGTCCGGTGCGCTAGAAACCATTTATGACCGCATTGCCACCTACAAAGAAAAAGCAGAAGCGCTAAAG
TCGAAAATTAAAAAAGCTATGTTCTATCCTGTGGCTGTGATAGTGGTGGCTTTTATCGTGACCACTATCTTGCTCATATT
TGTGGTGCCGCAGTTTGAAGAGATCTTTAGTAGTTTTGGTGCCGAGTTGCCGGCCTTTACCCAATTTGTATTAACCATAT
CTCGTTTTCTGCAAGAATTCGGATTATTTGTCGCAGCAGGCTTAGTGGTGGCTGGGATCTTGTTTAGTAAAACCTATAAA
AAATCAAAACGTCTGCGTGACAGCCTTGACAGTAAAATGCTCAAAATCCCTGTGATTGGCGAAATTTTAAAAAAGGCATC
AGTGGCTCGTTTTAGTCGCACCTTAGCGACGACATTTGCTGCGGGTGTGCCGTTAATAGGGGCGTTAGACTCCGCTGCTG
GCGCCTCGGGAAATGCGGTTTTCCGTGACGCTATCTTGTTTGTTAAAAAAGAAGTTGCCGGTGGTACGCAAATGAACACT
GCTATGCGAGCCACAGCAGTGTTTCCCGATATGGTGACCCAAATGGTGGCGATTGGTGAAGAAGCCGGCGCTGTTGATGA
TATGTTGAGTAAAATTGCCACCATTTATGAAGCAGAAGTAGATGATATGGTTGATGGTCTCACAAGCTTACTTGAACCAA
TGATTATGGCGGTGTTAGGCGTGGTCATTGGTGGCTTAATTGTCGCTATGTATCTCCCTATTTTTGAAATGGGCAATGTA
GTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(68-190)

Type II secretion system (T2SS), protein F (T2SSF)

(271-393)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A857JMP1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

58.794

99.252

0.584
  pilC Acinetobacter baumannii D1279779

52.513

99.252

0.521
  pilC Acinetobacter baylyi ADP1

52.381

99.501

0.521
  pilC Legionella pneumophila strain ERS1305867

49.373

99.501

0.491
  pilC Vibrio cholerae strain A1552

44.828

100

0.454
  pilC Vibrio campbellii strain DS40M4

43.25

99.751

0.431
  pilG Neisseria meningitidis 44/76-A

42.75

99.751

0.426
  pilG Neisseria gonorrhoeae MS11

42.5

99.751

0.424
  pilC Thermus thermophilus HB27

37.94

99.252

0.377

Multiple sequence alignment