Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   GU333_RS10470 Genome accession   NZ_CP047630
Coordinates   2104867..2105808 (-) Length   313 a.a.
NCBI ID   WP_061774317.1    Uniprot ID   -
Organism   Lactococcus raffinolactis strain Lr_18_12S     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2099867..2110808
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GU333_RS10430 (GU333_10395) - 2100392..2101240 (-) 849 WP_061774323.1 aminotransferase class IV -
  GU333_RS10435 (GU333_10400) ispE 2101324..2102175 (-) 852 WP_061774322.1 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase -
  GU333_RS10440 (GU333_10405) comGG 2102180..2102464 (-) 285 WP_096039677.1 competence type IV pilus minor pilin ComGG -
  GU333_RS10445 (GU333_10410) comGF 2102476..2102919 (-) 444 WP_244165991.1 competence type IV pilus minor pilin ComGF -
  GU333_RS10450 (GU333_10415) comGE 2102894..2103199 (-) 306 WP_082785376.1 competence type IV pilus minor pilin ComGE -
  GU333_RS10455 (GU333_10420) comGD 2103171..2103620 (-) 450 WP_167838308.1 competence type IV pilus minor pilin ComGD -
  GU333_RS10460 (GU333_10425) comYC 2103571..2103891 (-) 321 WP_061774318.1 competence type IV pilus major pilin ComGC Machinery gene
  GU333_RS10465 (GU333_10430) comYB 2103900..2104985 (-) 1086 WP_342591238.1 competence type IV pilus assembly protein ComGB Machinery gene
  GU333_RS10470 (GU333_10435) comGA/cglA/cilD 2104867..2105808 (-) 942 WP_061774317.1 competence type IV pilus ATPase ComGA Machinery gene
  GU333_RS10475 (GU333_10440) - 2105871..2107715 (-) 1845 WP_167842442.1 acyltransferase family protein -
  GU333_RS10480 (GU333_10445) - 2107712..2108074 (-) 363 WP_061774315.1 hypothetical protein -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 34933.21 Da        Isoelectric Point: 9.1747

>NTDB_id=414757 GU333_RS10470 WP_061774317.1 2104867..2105808(-) (comGA/cglA/cilD) [Lactococcus raffinolactis strain Lr_18_12S]
MIKEIAQRMLKKASDFGASDIYILPARTGFSVVFRKSAHREYDQLLSDAEGQSLISHFKFTAGMNVGEKRRPQLGSCLYE
LADRKCRLRLSSAGDFESRESLVIRILHDTKQPLKFWIEADLPQVKKLVARRGLYLFAGPVGSGKTTLMHHIAKEKFSGQ
QVITIEDPVEIVAPDLLQFQLNEAIGNTYDSLIKLSLRHMPDLVIVGEIRDQETARAVMRASLTGYTVFSTIHAKSIAGV
YARLLELGVTKEEINNSLSGVVYQRLIAGKGVLDSAEKAFERHANDKWHAKIEKLVTEGHLTPDQATAEKVSD

Nucleotide


Download         Length: 942 bp        

>NTDB_id=414757 GU333_RS10470 WP_061774317.1 2104867..2105808(-) (comGA/cglA/cilD) [Lactococcus raffinolactis strain Lr_18_12S]
ATGATAAAGGAAATAGCGCAAAGGATGTTAAAGAAAGCAAGCGATTTTGGGGCAAGTGATATTTATATATTACCCGCCAG
AACAGGATTTTCGGTTGTTTTCAGAAAGTCAGCACATCGTGAATATGATCAGCTCCTGTCAGATGCTGAAGGTCAAAGTC
TCATTTCCCATTTCAAATTTACAGCAGGGATGAATGTAGGTGAGAAGCGCCGGCCACAACTAGGATCTTGCTTGTATGAG
CTTGCAGATAGAAAGTGTCGCTTGCGTCTATCCTCAGCGGGTGACTTTGAAAGTCGCGAAAGTTTGGTGATTCGAATTTT
GCATGACACGAAACAACCACTCAAGTTTTGGATTGAGGCAGATTTACCTCAGGTAAAAAAGTTAGTAGCTAGACGCGGTC
TTTATTTATTTGCGGGGCCAGTTGGTTCTGGGAAAACGACGCTGATGCATCACATTGCCAAAGAAAAGTTTTCTGGTCAG
CAAGTCATTACGATTGAAGATCCAGTTGAAATCGTTGCACCAGATCTGCTCCAATTCCAACTGAATGAAGCGATTGGCAA
CACCTATGATAGCTTGATTAAATTATCCTTGCGCCATATGCCAGATCTTGTGATTGTCGGAGAAATTCGAGATCAGGAAA
CTGCGCGAGCAGTCATGAGGGCCAGTCTGACGGGCTATACTGTTTTTTCGACGATTCATGCTAAGTCGATTGCGGGTGTT
TATGCGCGATTATTGGAGCTTGGCGTAACAAAAGAAGAAATCAATAATTCGTTGTCAGGTGTTGTCTATCAACGGTTGAT
TGCAGGAAAGGGGGTGCTAGATAGTGCTGAAAAAGCATTCGAAAGGCATGCCAACGACAAGTGGCATGCCAAAATTGAAA
AATTGGTTACAGAAGGACATCTCACACCTGATCAGGCGACAGCCGAAAAGGTTAGCGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

57.692

99.681

0.575
  comGA/cglA/cilD Streptococcus pneumoniae R6

56.731

99.681

0.565
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

56.731

99.681

0.565
  comGA/cglA/cilD Streptococcus pneumoniae D39

56.731

99.681

0.565
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

56.731

99.681

0.565
  comGA Lactococcus lactis subsp. cremoris KW2

56.23

100

0.562
  comYA Streptococcus gordonii str. Challis substr. CH1

55.911

100

0.559
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

53.674

100

0.537
  comYA Streptococcus mutans UA140

52.548

100

0.527
  comYA Streptococcus mutans UA159

52.548

100

0.527

Multiple sequence alignment