Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   GT360_RS12145 Genome accession   NZ_CP047475
Coordinates   2623115..2624341 (+) Length   408 a.a.
NCBI ID   WP_164649132.1    Uniprot ID   A0A7Z2T4X1
Organism   Vibrio astriarenae strain HN897     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2618115..2629341
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GT360_RS12120 (GT360_12130) fldB 2618544..2619062 (+) 519 WP_164649128.1 flavodoxin FldB -
  GT360_RS12125 (GT360_12135) ampD 2619118..2619660 (-) 543 WP_164649129.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GT360_RS12130 (GT360_12140) nadC 2619792..2620679 (+) 888 WP_164649130.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GT360_RS12135 (GT360_12145) - 2620949..2621380 (+) 432 WP_164649651.1 pilin -
  GT360_RS12140 (GT360_12150) pilB 2621380..2623065 (+) 1686 WP_164649131.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GT360_RS12145 (GT360_12155) pilC 2623115..2624341 (+) 1227 WP_164649132.1 type II secretion system F family protein Machinery gene
  GT360_RS12150 (GT360_12160) pilD 2624438..2625304 (+) 867 WP_164649133.1 prepilin peptidase Machinery gene
  GT360_RS12155 (GT360_12165) coaE 2625309..2625929 (+) 621 WP_164649134.1 dephospho-CoA kinase -
  GT360_RS12160 (GT360_12170) zapD 2625954..2626694 (+) 741 WP_164649135.1 cell division protein ZapD -
  GT360_RS12165 (GT360_12175) yacG 2626742..2626939 (+) 198 WP_164649136.1 DNA gyrase inhibitor YacG -
  GT360_RS12170 (GT360_12180) rplS 2627023..2627376 (-) 354 WP_164649137.1 50S ribosomal protein L19 -
  GT360_RS12175 (GT360_12185) trmD 2627422..2628174 (-) 753 WP_164649138.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -
  GT360_RS12180 (GT360_12190) rimM 2628199..2628729 (-) 531 WP_164649139.1 ribosome maturation factor RimM -
  GT360_RS12185 (GT360_12195) rpsP 2628766..2629014 (-) 249 WP_005379962.1 30S ribosomal protein S16 -

Sequence

Protein


Download         Length: 408 a.a.        Molecular weight: 45072.33 Da        Isoelectric Point: 10.2669

>NTDB_id=413618 GT360_RS12145 WP_164649132.1 2623115..2624341(+) (pilC) [Vibrio astriarenae strain HN897]
MTVSSATQLKNYRWRGINSTGKKVSGQMLALNEVEVRDKLKEQHVSIKKIKRGSVSLLTKLTHRVNSKDITLLTRQLATM
LTTGVPIVQVLKLVADNHRKAEMKSILTQMSKGVEAGTPMSKAMRTASVHFDALYVDLVATGEQSGNLAEAFERLATYRE
KSEQLRAKVIKALIYPTMVLVVAMGVSYLMLTMVIPEFESMFKGFGAQLPWFTQKVLLLSHWIQAYSLWLVVTLCALCYI
GRLIRKQSDIIKLHTSRLSLKIPVIGEVLSKASIAKFSRTLATSFSAGIPILSSLKTSGKTSGNTHYQHAIDAVYRDTAA
GVPMYIAMRNTHAFPEMVLQMVMIGEESGKLDGMLNKVASIYEFEVDNTVDNLGKILEPLIIVFLGVVVGGLVVAMYLPI
FNLMSVLG

Nucleotide


Download         Length: 1227 bp        

>NTDB_id=413618 GT360_RS12145 WP_164649132.1 2623115..2624341(+) (pilC) [Vibrio astriarenae strain HN897]
ATGACAGTATCTTCAGCAACACAACTCAAAAACTATCGTTGGCGTGGCATCAACAGTACCGGGAAAAAAGTCTCGGGGCA
GATGCTCGCCCTTAATGAAGTGGAGGTACGAGATAAGCTCAAGGAACAGCATGTCAGTATTAAAAAGATCAAAAGAGGCA
GTGTTTCACTGCTGACGAAGCTAACGCATCGCGTGAACAGCAAAGACATCACCCTACTCACGCGACAATTAGCCACTATG
CTCACCACTGGTGTTCCTATCGTGCAAGTCCTCAAGCTTGTGGCTGATAATCATCGTAAAGCGGAAATGAAATCGATATT
GACGCAAATGAGTAAAGGCGTTGAAGCAGGGACTCCGATGTCAAAAGCAATGCGCACGGCCAGTGTTCATTTTGATGCGC
TATATGTTGATCTTGTCGCGACGGGGGAACAGTCCGGTAACTTAGCTGAAGCCTTTGAGCGCTTGGCTACCTATCGGGAG
AAGAGCGAACAGCTCCGAGCTAAAGTCATTAAAGCTCTGATTTATCCAACCATGGTGCTCGTGGTGGCGATGGGTGTCTC
CTATCTGATGCTGACTATGGTTATTCCAGAATTTGAGTCCATGTTTAAAGGCTTTGGTGCACAGCTGCCTTGGTTTACAC
AAAAAGTGCTTTTACTTTCTCACTGGATTCAAGCGTACAGCCTTTGGCTCGTTGTGACCCTTTGTGCACTTTGCTATATC
GGCCGACTCATTCGCAAACAGTCAGATATCATCAAGCTTCACACCAGTCGCCTTAGCCTAAAAATCCCCGTTATTGGAGA
GGTACTCAGTAAAGCGTCTATCGCCAAATTCAGCCGTACCTTAGCCACAAGTTTTAGTGCTGGAATCCCAATCCTCTCAA
GCCTCAAGACCTCCGGAAAAACATCTGGCAATACCCACTACCAACATGCCATTGATGCTGTGTACCGCGATACTGCAGCT
GGCGTGCCTATGTATATTGCGATGCGTAACACACATGCCTTTCCTGAGATGGTATTGCAGATGGTGATGATTGGTGAAGA
ATCGGGAAAACTTGATGGCATGCTAAATAAAGTGGCGTCCATTTATGAGTTTGAAGTCGATAACACCGTCGACAACCTCG
GTAAGATTCTTGAGCCCTTGATTATTGTGTTTCTCGGCGTTGTGGTTGGTGGGTTAGTGGTCGCGATGTATTTACCGATC
TTTAACCTAATGAGTGTGTTAGGATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(74-196)

Type II secretion system (T2SS), protein F (T2SSF)

(277-399)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7Z2T4X1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio cholerae strain A1552

79.902

100

0.799
  pilC Vibrio campbellii strain DS40M4

72.457

98.775

0.716
  pilC Acinetobacter baumannii D1279779

40.976

100

0.412
  pilC Legionella pneumophila strain ERS1305867

40.695

98.775

0.402
  pilC Acinetobacter baylyi ADP1

40.351

97.794

0.395
  pilG Neisseria meningitidis 44/76-A

38.916

99.51

0.387
  pilG Neisseria gonorrhoeae MS11

38.614

99.02

0.382
  pilC Pseudomonas stutzeri DSM 10701

38.889

97.059

0.377

Multiple sequence alignment