Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GT360_RS12140 Genome accession   NZ_CP047475
Coordinates   2621380..2623065 (+) Length   561 a.a.
NCBI ID   WP_164649131.1    Uniprot ID   A0A7Z2T4U4
Organism   Vibrio astriarenae strain HN897     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2616380..2628065
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GT360_RS12110 (GT360_12120) - 2616687..2617469 (-) 783 WP_164649127.1 thioredoxin fold domain-containing protein -
  GT360_RS12115 (GT360_12125) xerD 2617497..2618393 (-) 897 WP_164649650.1 site-specific tyrosine recombinase XerD -
  GT360_RS12120 (GT360_12130) fldB 2618544..2619062 (+) 519 WP_164649128.1 flavodoxin FldB -
  GT360_RS12125 (GT360_12135) ampD 2619118..2619660 (-) 543 WP_164649129.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GT360_RS12130 (GT360_12140) nadC 2619792..2620679 (+) 888 WP_164649130.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GT360_RS12135 (GT360_12145) - 2620949..2621380 (+) 432 WP_164649651.1 pilin -
  GT360_RS12140 (GT360_12150) pilB 2621380..2623065 (+) 1686 WP_164649131.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GT360_RS12145 (GT360_12155) pilC 2623115..2624341 (+) 1227 WP_164649132.1 type II secretion system F family protein Machinery gene
  GT360_RS12150 (GT360_12160) pilD 2624438..2625304 (+) 867 WP_164649133.1 prepilin peptidase Machinery gene
  GT360_RS12155 (GT360_12165) coaE 2625309..2625929 (+) 621 WP_164649134.1 dephospho-CoA kinase -
  GT360_RS12160 (GT360_12170) zapD 2625954..2626694 (+) 741 WP_164649135.1 cell division protein ZapD -
  GT360_RS12165 (GT360_12175) yacG 2626742..2626939 (+) 198 WP_164649136.1 DNA gyrase inhibitor YacG -
  GT360_RS12170 (GT360_12180) rplS 2627023..2627376 (-) 354 WP_164649137.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62072.10 Da        Isoelectric Point: 6.3249

>NTDB_id=413617 GT360_RS12140 WP_164649131.1 2621380..2623065(+) (pilB) [Vibrio astriarenae strain HN897]
MPTNLPSILRQANLISLAQEKQVFEYIQSQGGSAPQAMLDLHLFAPSELTEHISQLFGLPEIQLTRYTYAGLCQQLGLRE
LICRYQTLPISSDGHTLTLAVSDPTYNEVEDEFRFATGQQVEMVLADHLALKAAIRRLYGSSLENSQSNIKEISQEELVD
LVDIGEDELDNMEDLSQDDSPVSRFINQILLDAVRKKASDIHFEPYEKSYRIRLRCDGLLVEVQQPSSQLSRRLSARIKI
LAKLDIAERRLPQDGRIKLRLNTHTAVDMRVSSLPTLWGEKIVLRILDSSVASLDIDKLGYNTAQKQRYMDALKKPQGMI
LMTGPTGSGKTVSLYAGLKVLNTPEVNISTAEDPVEINLAGINQVQVQPKIGFGFAQALRSFLRQDPDIVMVGEVRDKET
AEIAVKAAQTGHLVLSTLHTNSAAESIFRLSNMGIEPYNLASSLSLIIAQRLARKLCPQCKQPHQNVSLLNAVGIHDTHG
IYQAQPSGCNECNSGYAGRIGIYEVMAFNQALVDALLKRAGVNEIESIAIANGMSTLKQSGIEKLKQGVTSLHELQRVLH
L

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=413617 GT360_RS12140 WP_164649131.1 2621380..2623065(+) (pilB) [Vibrio astriarenae strain HN897]
ATGCCAACTAACCTACCCTCAATCTTGCGCCAAGCCAATCTCATCAGCTTGGCGCAGGAAAAGCAGGTGTTCGAGTATAT
TCAAAGCCAAGGTGGCAGCGCACCTCAGGCGATGCTCGACCTTCACCTGTTTGCTCCCAGTGAGCTCACCGAGCACATCA
GCCAGCTATTTGGCTTGCCGGAAATCCAGCTTACTCGTTATACATACGCTGGACTTTGCCAACAATTAGGACTGCGTGAG
CTAATTTGCCGCTATCAGACTTTACCGATATCCAGCGATGGTCACACTCTCACCCTGGCCGTTTCAGATCCGACATACAA
TGAAGTAGAAGACGAGTTTCGTTTTGCTACGGGGCAGCAAGTGGAAATGGTTTTGGCCGATCATCTTGCCTTGAAGGCTG
CCATCCGGCGACTTTATGGTTCATCTTTGGAAAACTCGCAATCCAATATCAAAGAGATCAGCCAAGAAGAGCTGGTTGAT
CTCGTCGATATTGGTGAAGATGAACTCGATAACATGGAAGATCTTAGCCAAGACGACTCCCCCGTGAGCCGCTTCATCAA
TCAAATTCTGCTCGACGCTGTGCGCAAGAAAGCCTCTGATATTCATTTCGAGCCCTATGAAAAGAGCTATCGGATCCGTT
TACGCTGTGATGGTCTCTTAGTCGAAGTGCAGCAGCCATCATCACAATTGAGTCGCCGACTCTCTGCTCGCATAAAAATC
CTCGCCAAACTGGATATCGCTGAGAGGCGCTTACCACAAGACGGTCGCATCAAGCTGCGCCTTAACACGCATACCGCTGT
TGATATGCGTGTCTCCAGTCTACCGACACTATGGGGAGAGAAGATCGTCCTGAGGATATTAGATAGTAGCGTAGCAAGTT
TAGATATTGACAAGTTAGGGTATAACACCGCCCAGAAACAGCGCTATATGGATGCCCTAAAAAAGCCACAAGGCATGATC
TTAATGACCGGGCCAACAGGCAGTGGCAAAACCGTTTCACTGTATGCTGGCCTCAAGGTCCTCAACACACCGGAAGTCAA
TATCTCCACCGCTGAAGACCCAGTAGAGATTAACCTTGCGGGGATCAATCAAGTACAGGTGCAGCCCAAAATCGGCTTTG
GGTTTGCTCAGGCACTGCGATCATTCCTGCGTCAAGATCCGGATATCGTGATGGTTGGAGAGGTGCGAGATAAAGAGACG
GCTGAAATCGCCGTCAAGGCGGCGCAAACTGGGCATCTAGTGCTTTCAACCCTGCACACTAACTCAGCTGCGGAGTCGAT
ATTCCGCCTTAGTAATATGGGTATAGAACCCTATAATCTCGCCTCATCGCTTAGCTTGATAATAGCGCAACGCTTGGCTA
GAAAGCTCTGCCCTCAATGCAAACAACCGCATCAAAACGTCTCCCTACTCAATGCTGTTGGCATCCACGACACTCATGGG
ATTTACCAAGCTCAACCCAGTGGCTGTAATGAGTGTAACTCCGGTTACGCAGGACGTATTGGTATCTATGAAGTCATGGC
GTTTAATCAAGCATTGGTTGATGCACTACTCAAACGAGCCGGTGTGAATGAAATAGAGTCCATTGCCATAGCCAATGGTA
TGAGTACCCTCAAACAGTCTGGTATCGAGAAACTAAAACAAGGCGTCACTAGCCTACATGAACTTCAGCGCGTGCTTCAC
CTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-143)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7Z2T4U4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio parahaemolyticus RIMD 2210633

70.232

100

0.702
  pilB Vibrio cholerae strain A1552

70.107

100

0.702
  pilB Vibrio campbellii strain DS40M4

69.519

100

0.695
  pilB Acinetobacter baylyi ADP1

49.905

94.296

0.471
  pilB Acinetobacter baumannii D1279779

50

93.761

0.469
  pilB Legionella pneumophila strain ERS1305867

48.638

91.622

0.446
  pilF Neisseria gonorrhoeae MS11

43.894

100

0.442
  pilB/pilB1 Synechocystis sp. PCC 6803

39.382

92.335

0.364
  pilF Thermus thermophilus HB27

36.462

98.752

0.36

Multiple sequence alignment