Detailed information NTG0413619

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: GT360_RS12150
• Genome accession: NZ_CP047475
• Coordinates: 2624438..2625304 (+)
• Length: 288 a.a.
• NCBI ID: WP_164649133.1
• Uniprot ID: A0A7Z2T4G3
• Organism: Vibrio astriarenae strain HN897
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2619438..2630304

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GT360_RS12130 (GT360_12140)	nadC	2619792..2620679 (+)	888	WP_164649130.1	carboxylating nicotinate-nucleotide diphosphorylase	-
GT360_RS12135 (GT360_12145)	-	2620949..2621380 (+)	432	WP_164649651.1	pilin	-
GT360_RS12140 (GT360_12150)	pilB	2621380..2623065 (+)	1686	WP_164649131.1	type IV-A pilus assembly ATPase PilB	Machinery gene
GT360_RS12145 (GT360_12155)	pilC	2623115..2624341 (+)	1227	WP_164649132.1	type II secretion system F family protein	Machinery gene
GT360_RS12150 (GT360_12160)	pilD	2624438..2625304 (+)	867	WP_164649133.1	prepilin peptidase	Machinery gene
GT360_RS12155 (GT360_12165)	coaE	2625309..2625929 (+)	621	WP_164649134.1	dephospho-CoA kinase	-
GT360_RS12160 (GT360_12170)	zapD	2625954..2626694 (+)	741	WP_164649135.1	cell division protein ZapD	-
GT360_RS12165 (GT360_12175)	yacG	2626742..2626939 (+)	198	WP_164649136.1	DNA gyrase inhibitor YacG	-
GT360_RS12170 (GT360_12180)	rplS	2627023..2627376 (-)	354	WP_164649137.1	50S ribosomal protein L19	-
GT360_RS12175 (GT360_12185)	trmD	2627422..2628174 (-)	753	WP_164649138.1	tRNA (guanosine(37)-N1)-methyltransferase TrmD	-
GT360_RS12180 (GT360_12190)	rimM	2628199..2628729 (-)	531	WP_164649139.1	ribosome maturation factor RimM	-
GT360_RS12185 (GT360_12195)	rpsP	2628766..2629014 (-)	249	WP_005379962.1	30S ribosomal protein S16	-

Sequence

Protein

• Length: 288 a.a.
• Molecular weight: 32411.79 Da
• Isoelectric Point: 8.2022

>NTDB_id=413619 GT360_RS12150 WP_164649133.1 2624438..2625304(+) (pilD) [Vibrio astriarenae strain HN897]
MDVFIYYPWLFVVFATVFGLIVGSFLNVVIFRLPKMMELDWRRECAEYFPEYKIPKPTETLTLSKPNSTCQSCKTPIRII
DNIPVISWLLLRGRCHKCKSKISPRYPLIETLTAILCYLVATHFGFSGYGVALLFFTFVLIAATFIDLDTMLLPDQLTLP
LMWGGIALALFEISPVTLQDSVIGAMAGYLSLWSIYWGFKLLTGKEGMGYGDFKLLAALGAWLGWQALPMIILLSSVVGL
IFGIIQLRLQKQGIEKAFPFGPYLAIAGWVAMLWGEQILSVYWGYVLG

Nucleotide

• Length: 867 bp

>NTDB_id=413619 GT360_RS12150 WP_164649133.1 2624438..2625304(+) (pilD) [Vibrio astriarenae strain HN897]
ATGGATGTGTTTATCTACTACCCGTGGTTATTTGTGGTTTTCGCTACGGTGTTTGGTCTCATTGTTGGCAGCTTTTTAAA
TGTGGTGATCTTCCGTCTACCCAAAATGATGGAGCTTGACTGGCGTAGAGAATGCGCCGAGTATTTCCCTGAATACAAGA
TTCCAAAACCCACAGAAACACTCACGCTAAGTAAACCAAACTCAACGTGCCAGAGCTGCAAAACCCCTATCCGCATCATT
GATAACATTCCTGTGATTAGCTGGCTCTTGCTGCGCGGGCGCTGCCACAAATGCAAAAGCAAGATAAGCCCTCGCTACCC
ACTGATTGAAACCTTAACCGCCATACTCTGCTATTTAGTTGCGACACATTTTGGCTTTAGTGGCTATGGGGTAGCGCTGC
TATTTTTTACCTTCGTCCTCATCGCAGCCACGTTTATCGATCTCGATACCATGCTGCTACCGGATCAACTGACGTTACCC
TTGATGTGGGGAGGTATTGCGCTCGCGTTATTTGAGATCAGCCCAGTCACATTGCAAGACAGCGTCATCGGAGCCATGGC
AGGCTATCTATCACTCTGGAGCATCTATTGGGGCTTCAAACTCCTTACTGGTAAAGAGGGGATGGGCTATGGTGATTTCA
AACTACTTGCCGCTCTTGGCGCATGGCTTGGTTGGCAAGCACTACCCATGATCATCCTGCTCTCTTCTGTTGTCGGACTC
ATCTTCGGCATCATTCAATTACGCCTGCAGAAACAGGGGATTGAGAAAGCCTTCCCATTTGGGCCATATCTTGCTATCGC
TGGTTGGGTGGCGATGCTGTGGGGAGAGCAAATCTTGTCTGTCTACTGGGGTTACGTTTTAGGATAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (135-244)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (17-124)

3D structure

• AlphaFold DB: A0A7Z2T4G3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio campbellii strain DS40M4	80.208	100	0.802
pilD	Vibrio cholerae strain A1552	73.05	97.917	0.715
pilD	Acinetobacter baumannii D1279779	52.708	96.181	0.507
pilD	Acinetobacter nosocomialis M2	52.347	96.181	0.503
pilD	Neisseria gonorrhoeae MS11	50.579	89.931	0.455