Detailed information NTG0413041

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: H4P48_RS03955
• Genome accession: NZ_CP060073
• Coordinates: 827616..828596 (+)
• Length: 326 a.a.
• NCBI ID: WP_001295381.1
• Uniprot ID: -
• Organism: Escherichia coli strain G4/9
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 822616..833596

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H4P48_RS03925 (H4P48_03925)	yggM	823131..824138 (+)	1008	WP_000745217.1	DUF1202 family protein	-
H4P48_RS03930 (H4P48_03930)	hemW	824293..825429 (-)	1137	WP_047643745.1	radical SAM family heme chaperone HemW	-
H4P48_RS03935 (H4P48_03935)	rdgB	825422..826015 (-)	594	WP_001174735.1	XTP/dITP diphosphatase	-
H4P48_RS03940 (H4P48_03940)	yggU	826023..826313 (-)	291	WP_001277222.1	DUF167 family protein YggU	-
H4P48_RS03945 (H4P48_03945)	yggT	826310..826876 (-)	567	WP_001094831.1	osmotic shock tolerance protein YggT	-
H4P48_RS03950 (H4P48_03950)	yggS	826894..827598 (-)	705	WP_000997795.1	pyridoxal phosphate homeostasis protein	-
H4P48_RS03955 (H4P48_03955)	pilT	827616..828596 (+)	981	WP_001295381.1	type IV pilus twitching motility protein PilT	Machinery gene
H4P48_RS22530	-	828645..828773 (+)	129	WP_001278270.1	hypothetical protein	-
H4P48_RS03960 (H4P48_03960)	ruvX	828780..829196 (-)	417	WP_000017106.1	Holliday junction resolvase RuvX	-
H4P48_RS03965 (H4P48_03965)	yqgE	829196..829759 (-)	564	WP_001053178.1	YqgE/AlgH family protein	-
H4P48_RS03970 (H4P48_03970)	gshB	829868..830818 (-)	951	WP_000593273.1	glutathione synthase	-
H4P48_RS03975 (H4P48_03975)	rsmE	830831..831562 (-)	732	WP_001222509.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
H4P48_RS03980 (H4P48_03980)	endA	831642..832349 (-)	708	WP_000286500.1	deoxyribonuclease I	-
H4P48_RS03985 (H4P48_03985)	yggI	832444..832941 (-)	498	WP_001300769.1	SprT family zinc-dependent metalloprotease	-

Sequence

Protein

• Length: 326 a.a.
• Molecular weight: 35926.14 Da
• Isoelectric Point: 5.7654

>NTDB_id=413041 H4P48_RS03955 WP_001295381.1 827616..828596(+) (pilT) [Escherichia coli strain G4/9]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGMILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQHR
VGEGRL

Nucleotide

• Length: 981 bp

>NTDB_id=413041 H4P48_RS03955 WP_001295381.1 827616..828596(+) (pilT) [Escherichia coli strain G4/9]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGGCTAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGCCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCATGATTCTGGTGACGGGGGCGACGGGGAGTGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTTAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGGTTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTACGTGACAGTGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCAACCTTACATACACGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGACCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCAGTGCTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTATTTGAATTGCTGATTAACACACCCGCGGTGGGGAATTTGATTCGTGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCACCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (62-239)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Neisseria meningitidis 8013	49.085	100	0.494
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	48.93	100	0.491
pilT	Vibrio cholerae strain A1552	48.93	100	0.491
pilT	Neisseria gonorrhoeae MS11	48.78	100	0.491
pilT	Acinetobacter baumannii D1279779	46.483	100	0.466
pilT	Acinetobacter nosocomialis M2	46.483	100	0.466
pilT	Acinetobacter baumannii strain A118	46.483	100	0.466
pilT	Pseudomonas stutzeri DSM 10701	46.177	100	0.463
pilT	Acinetobacter baylyi ADP1	45.872	100	0.46
pilT	Pseudomonas aeruginosa PAK	45.566	100	0.457
pilT	Legionella pneumophila strain ERS1305867	45.912	97.546	0.448
pilT	Legionella pneumophila strain Lp02	45.912	97.546	0.448
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.824	97.546	0.408
pilU	Vibrio cholerae strain A1552	40.062	98.773	0.396
pilU	Pseudomonas stutzeri DSM 10701	37.273	100	0.377
pilU	Acinetobacter baylyi ADP1	37.048	100	0.377
pilB	Legionella pneumophila strain ERS1305867	30.89	100	0.362