Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GTH25_RS12750 Genome accession   NZ_CP047349
Coordinates   2729977..2731077 (+) Length   366 a.a.
NCBI ID   WP_164530616.1    Uniprot ID   -
Organism   Proteus terrae subsp. cibarius strain ZN2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2724977..2736077
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GTH25_RS12735 (GTH25_12660) pdhR 2727583..2728353 (-) 771 WP_164530615.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  GTH25_RS12740 (GTH25_12665) ampD 2728679..2729221 (-) 543 WP_075670826.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GTH25_RS12745 (GTH25_12670) ppdD 2729432..2729875 (+) 444 WP_075670824.1 prepilin peptidase-dependent pilin -
  GTH25_RS12750 (GTH25_12675) pilB 2729977..2731077 (+) 1101 WP_164530616.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  GTH25_RS12755 (GTH25_12680) - 2731074..2732273 (+) 1200 WP_156733816.1 type II secretion system F family protein -
  GTH25_RS12760 (GTH25_12685) coaE 2732508..2733131 (+) 624 WP_075670818.1 dephospho-CoA kinase -
  GTH25_RS12765 (GTH25_12690) zapD 2733124..2733876 (+) 753 WP_075670816.1 cell division protein ZapD -
  GTH25_RS12770 (GTH25_12695) yacG 2733931..2734143 (+) 213 WP_075670814.1 DNA gyrase inhibitor YacG -
  GTH25_RS12775 (GTH25_12700) mutT 2734230..2734622 (-) 393 WP_075670812.1 8-oxo-dGTP diphosphatase MutT -
  GTH25_RS12780 (GTH25_12705) - 2734729..2735979 (-) 1251 WP_099659318.1 aromatic amino acid transporter -

Sequence

Protein


Download         Length: 366 a.a.        Molecular weight: 41463.02 Da        Isoelectric Point: 7.6442

>NTDB_id=412873 GTH25_RS12750 WP_164530616.1 2729977..2731077(+) (pilB) [Proteus terrae subsp. cibarius strain ZN2]
MDITHSATEFIENLLRTSILKRVSDLHIEPQQISVRIRARIDNHLYLFSPPPNEFSEEIVTRLKVLANLNIAEKRLPQDG
QFNWSYHEKNYSIRIATLPTLYGEKVVLRLINNLQQPELNHLGFQPSHLTLLKKYLNLPQGMILVTGPTGSGKTLTLYSC
LRYLNKENLNINSVEDPVELPLKGINQIQISEKSGISFTTILRALLRQDPDIIMVGEIRDQSTAEMAIKAAQTGHLVLST
LHTNSASSTLMRLYNLGIEKDLIHSCVSLIISQRLVRCLCPLCKICLPNKQHININKEMIEIPNWQAVGCQQCYSGYNGR
TAIYDCFEPLKQTLSSPYHLLYSGISLIKQGITTLEEVYQTLGDIE

Nucleotide


Download         Length: 1101 bp        

>NTDB_id=412873 GTH25_RS12750 WP_164530616.1 2729977..2731077(+) (pilB) [Proteus terrae subsp. cibarius strain ZN2]
ATGGATATCACACACTCTGCAACAGAATTTATTGAGAATCTATTACGTACAAGTATTTTAAAGCGTGTCTCTGATTTACA
TATAGAGCCACAGCAAATCAGTGTAAGAATTCGAGCCAGAATAGATAATCATTTATATCTATTTTCGCCACCTCCAAATG
AATTCTCTGAAGAAATAGTGACTCGCTTAAAGGTACTCGCTAACTTAAATATTGCAGAGAAGCGATTGCCTCAAGATGGG
CAGTTTAATTGGTCTTATCATGAAAAAAACTATTCAATACGAATTGCGACACTTCCCACATTATATGGCGAAAAAGTAGT
TTTACGGCTTATCAATAATTTACAACAACCGGAATTAAATCACCTGGGCTTTCAACCCTCACATTTAACGCTATTAAAAA
AATATTTAAATTTACCGCAAGGAATGATTTTAGTTACAGGGCCTACGGGAAGCGGAAAAACGCTCACACTATATAGTTGT
TTACGTTATTTAAATAAAGAAAACCTAAATATAAATAGTGTTGAAGATCCGGTTGAATTACCTCTAAAGGGTATTAATCA
GATCCAAATTTCCGAAAAATCAGGGATCTCATTCACAACAATATTACGGGCATTACTACGCCAAGATCCTGACATTATTA
TGGTAGGTGAAATACGTGATCAATCGACTGCTGAAATGGCAATAAAAGCAGCACAAACAGGGCATCTTGTATTATCAACC
TTACATACTAATTCGGCTTCATCCACATTAATGCGTCTATATAACTTAGGAATTGAAAAAGATCTTATTCACTCCTGTGT
TAGCTTAATTATTTCACAACGATTAGTTCGTTGTTTATGTCCACTCTGCAAAATATGTCTACCCAATAAACAGCATATAA
ATATAAACAAAGAAATGATCGAAATACCAAACTGGCAAGCTGTTGGTTGCCAGCAATGCTATTCAGGCTATAACGGAAGA
ACGGCTATTTATGACTGCTTTGAGCCCTTAAAACAAACTCTCTCATCCCCTTATCACTTACTCTATTCCGGCATTTCATT
AATTAAACAAGGAATAACGACACTTGAGGAGGTTTATCAAACACTAGGAGATATTGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(16-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

46.438

100

0.481
  pilB Legionella pneumophila strain ERS1305867

45.288

100

0.473
  pilB Vibrio parahaemolyticus RIMD 2210633

45

100

0.467
  pilB Vibrio cholerae strain A1552

43.421

100

0.451
  pilB Acinetobacter baylyi ADP1

43.386

100

0.448
  pilF Neisseria gonorrhoeae MS11

42.228

100

0.445
  pilB Acinetobacter baumannii D1279779

42.706

100

0.44
  pilB Glaesserella parasuis strain SC1401

42.74

99.727

0.426
  pilF Thermus thermophilus HB27

39.153

100

0.404
  pilB Haemophilus influenzae 86-028NP

40.608

98.907

0.402
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.889

100

0.402
  ctsE Campylobacter jejuni subsp. jejuni 81-176

40.556

98.361

0.399
  pilB/pilB1 Synechocystis sp. PCC 6803

37.436

100

0.399
  pilB Haemophilus influenzae Rd KW20

40.331

98.907

0.399

Multiple sequence alignment