Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   H5023_RS05980 Genome accession   NZ_CP060005
Coordinates   1286343..1287560 (-) Length   405 a.a.
NCBI ID   WP_003116250.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain WK190     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1281343..1292560
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H5023_RS05935 (H5023_05930) - 1281525..1281974 (-) 450 WP_003094670.1 MOSC domain-containing protein -
  H5023_RS05940 (H5023_05935) - 1281971..1282600 (-) 630 WP_003094668.1 DUF1780 domain-containing protein -
  H5023_RS05945 (H5023_05940) - 1282733..1283158 (+) 426 WP_003103865.1 GNAT family N-acetyltransferase -
  H5023_RS05950 (H5023_05945) - 1283155..1283625 (+) 471 WP_003094664.1 hypothetical protein -
  H5023_RS05955 (H5023_05950) - 1283622..1284311 (+) 690 WP_003094662.1 energy-coupling factor ABC transporter permease -
  H5023_RS05960 (H5023_05955) - 1284417..1284626 (+) 210 WP_003120946.1 hypothetical protein -
  H5023_RS05965 (H5023_05960) yacG 1284663..1284863 (-) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  H5023_RS05970 (H5023_05965) coaE 1284860..1285471 (-) 612 WP_033982615.1 dephospho-CoA kinase -
  H5023_RS05975 (H5023_05970) pilD 1285468..1286340 (-) 873 WP_016562340.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  H5023_RS05980 (H5023_05975) pilC 1286343..1287560 (-) 1218 WP_003116250.1 type II secretion system F family protein Machinery gene
  H5023_RS05985 (H5023_05980) pilB 1287564..1289267 (-) 1704 WP_003116249.1 type IV-A pilus assembly ATPase PilB Machinery gene
  H5023_RS05990 (H5023_05985) pilE 1289496..1290035 (+) 540 WP_003116248.1 pilin Machinery gene
  H5023_RS05995 (H5023_05990) - 1290099..1290797 (+) 699 WP_023122772.1 hypothetical protein -
  H5023_RS06005 (H5023_06000) nadC 1290962..1291810 (-) 849 WP_003116246.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44051.94 Da        Isoelectric Point: 9.8417

>NTDB_id=412396 H5023_RS05980 WP_003116250.1 1286343..1287560(-) (pilC) [Pseudomonas aeruginosa strain WK190]
MADKALKTSVFIWEGTDKKGSKVKGELAGQNPMLVKAQLRKQGINPLKVRKKGITLFGAGKKIKPMDIALFTRQMSTMMG
AGVPLLQSFDIIGEGFENPNMRKLVDEIKQDVAAGNSLASSLRKKPIYFDDLYCNLVDAGEQSGALETLLDRVATYKEKT
ESLKAKIKKAMTYPIAVIVVALVVSAILLIKVVPQFQSVFANFGAELPAFTQMVINLSEILQEWWLIVLIGLFAAAFAFR
EVHRRSQKLRDSVDRGLLKLPIIGGILYKSAIARYARTLSTTFAAGVPLVEALDSVSGATGNVVFKNAVTKIKQDVSSGM
QLNFSMRTTNVFPSMAIQMAAIGEESGSLDEMLGKVAGFYEEEVDNAVDNLTTLMEPMIMAVLGVLVGGLIIAMYLPIFQ
LGSVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=412396 H5023_RS05980 WP_003116250.1 1286343..1287560(-) (pilC) [Pseudomonas aeruginosa strain WK190]
ATGGCGGACAAAGCGTTAAAGACCAGTGTCTTCATCTGGGAAGGCACTGATAAGAAAGGCAGCAAGGTCAAAGGAGAGTT
GGCCGGGCAAAACCCTATGCTGGTGAAGGCTCAACTGCGCAAACAGGGTATCAACCCACTAAAGGTCCGCAAGAAAGGTA
TCACCCTGTTTGGGGCAGGGAAGAAGATTAAGCCCATGGACATCGCCCTGTTCACTCGGCAGATGTCTACCATGATGGGT
GCCGGCGTACCGTTACTGCAATCTTTTGACATCATCGGCGAAGGATTCGAAAATCCAAACATGCGCAAGCTAGTCGATGA
GATCAAGCAGGATGTTGCCGCCGGTAACAGCTTAGCCAGTTCACTTCGAAAGAAACCCATTTACTTCGATGATCTCTACT
GCAACCTGGTCGATGCTGGCGAACAGTCCGGTGCTTTGGAGACATTATTGGATCGGGTAGCAACTTATAAAGAAAAGACA
GAATCCCTGAAAGCCAAAATTAAAAAGGCCATGACTTATCCCATTGCAGTAATTGTAGTGGCCCTTGTGGTGTCGGCGAT
CCTTCTGATAAAAGTGGTCCCACAGTTCCAGTCCGTATTTGCAAATTTTGGCGCCGAGTTGCCGGCCTTTACTCAAATGG
TCATCAATCTTTCCGAGATTCTTCAAGAGTGGTGGCTCATAGTACTTATTGGTCTTTTTGCCGCAGCTTTTGCATTTAGG
GAAGTTCATCGGCGCTCGCAAAAACTGAGAGATTCAGTAGATCGGGGCCTGCTGAAACTACCTATCATCGGCGGGATACT
TTACAAATCAGCTATCGCCCGCTACGCCCGAACGCTATCCACTACCTTTGCGGCTGGAGTGCCTCTGGTAGAAGCTCTGG
ACTCCGTTTCCGGAGCAACTGGCAACGTGGTATTCAAGAATGCCGTTACCAAGATCAAACAAGACGTGTCCAGCGGCATG
CAATTGAACTTTTCCATGCGCACCACCAATGTTTTCCCCAGTATGGCTATCCAGATGGCTGCAATTGGCGAGGAATCAGG
TTCGCTAGATGAGATGTTAGGAAAAGTTGCCGGTTTCTATGAGGAAGAAGTCGATAACGCCGTCGACAACCTGACAACGT
TGATGGAGCCGATGATCATGGCCGTGCTCGGCGTACTGGTCGGCGGCCTGATCATCGCTATGTATCTACCGATCTTCCAA
CTGGGTTCCGTTGTGTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

79.506

100

0.795
  pilC Acinetobacter baumannii D1279779

60.644

99.753

0.605
  pilC Acinetobacter baylyi ADP1

59.606

100

0.598
  pilC Legionella pneumophila strain ERS1305867

55.051

97.778

0.538
  pilG Neisseria meningitidis 44/76-A

46.269

99.259

0.459
  pilG Neisseria gonorrhoeae MS11

46.02

99.259

0.457
  pilC Vibrio cholerae strain A1552

42.222

100

0.422
  pilC Vibrio campbellii strain DS40M4

40.909

97.778

0.4
  pilC Thermus thermophilus HB27

38.404

99.012

0.38