Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   GTF71_RS02300 Genome accession   NZ_CP047299
Coordinates   478992..479453 (-) Length   153 a.a.
NCBI ID   WP_000649326.1    Uniprot ID   A0A0X1KZH2
Organism   Vibrio cholerae O1 biovar El Tor strain P27459     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 473992..484453
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GTF71_RS02280 (GTF71_02280) coaE 474497..475105 (-) 609 WP_000011557.1 dephospho-CoA kinase -
  GTF71_RS02285 (GTF71_02285) pilD 475102..475977 (-) 876 WP_000418747.1 A24 family peptidase Machinery gene
  GTF71_RS02290 (GTF71_02290) pilC 476035..477261 (-) 1227 WP_000648511.1 type II secretion system F family protein Machinery gene
  GTF71_RS02295 (GTF71_02295) pilB 477304..478992 (-) 1689 WP_000957200.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GTF71_RS02300 (GTF71_02300) pilA 478992..479453 (-) 462 WP_000649326.1 pilin Machinery gene
  GTF71_RS02305 (GTF71_02305) nadC 479698..480588 (-) 891 WP_000665274.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GTF71_RS02310 (GTF71_02310) ampD 480884..481429 (+) 546 WP_000567318.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GTF71_RS02315 (GTF71_02315) fldB 481707..482225 (-) 519 WP_000690112.1 flavodoxin FldB -
  GTF71_RS02320 (GTF71_02320) xerD 482363..483271 (+) 909 WP_001287472.1 site-specific tyrosine recombinase XerD -
  GTF71_RS02325 (GTF71_02325) dsbC 483288..484040 (+) 753 WP_000115055.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -

Sequence

Protein


Download         Length: 153 a.a.        Molecular weight: 15722.14 Da        Isoelectric Point: 8.9860

>NTDB_id=412368 GTF71_RS02300 WP_000649326.1 478992..479453(-) (pilA) [Vibrio cholerae O1 biovar El Tor strain P27459]
MKAYKNKQQKGFTLIELMIVVAVIGVLAAIAIPQYQNYVKKSAIGVGLANITALKTNIEDYIATEGSFPATTAGTAAGFT
RLGTVEDMGDGKIVIAPTASGALGGTIKYTFDAGVVSSSKIQLARDANGLWTCSTTVTSEIAPKGCTAGATIN

Nucleotide


Download         Length: 462 bp        

>NTDB_id=412368 GTF71_RS02300 WP_000649326.1 478992..479453(-) (pilA) [Vibrio cholerae O1 biovar El Tor strain P27459]
ATGAAAGCGTATAAAAACAAACAACAGAAAGGTTTTACCTTAATTGAATTGATGATTGTGGTGGCAGTGATTGGTGTGTT
GGCAGCGATTGCAATTCCTCAATATCAAAATTATGTAAAAAAATCAGCTATTGGGGTTGGCTTAGCAAACATCACAGCAC
TGAAAACTAATATTGAAGATTATATTGCGACAGAAGGCTCTTTTCCTGCAACAACTGCAGGTACGGCCGCAGGATTTACA
CGCTTAGGTACTGTTGAAGACATGGGTGACGGTAAGATTGTAATTGCCCCAACAGCATCAGGTGCATTAGGAGGTACAAT
AAAATACACATTCGATGCTGGTGTAGTATCTAGTTCAAAAATCCAATTAGCAAGAGATGCCAATGGATTATGGACTTGTA
GTACCACCGTTACTAGTGAAATAGCACCAAAAGGCTGTACTGCAGGTGCAACAATTAACTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)

Pilin (bacterial filament) (Pilin)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0X1KZH2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

100

100

1
  pilA Vibrio cholerae strain A1552

100

100

1
  pilA Vibrio cholerae C6706

100

100

1
  pilA Pseudomonas aeruginosa PAK

42.675

100

0.438
  pilA Acinetobacter baumannii strain A118

42.667

98.039

0.418
  pilA Vibrio parahaemolyticus RIMD 2210633

41.892

96.732

0.405
  pilA/pilAI Pseudomonas stutzeri DSM 10701

39.86

93.464

0.373
  pilA2 Legionella pneumophila str. Paris

39.31

94.771

0.373
  pilA2 Legionella pneumophila strain ERS1305867

38.621

94.771

0.366

Multiple sequence alignment