Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   GSR16_RS17135 Genome accession   NZ_CP047241
Coordinates   3723719..3725431 (-) Length   570 a.a.
NCBI ID   WP_159879533.1    Uniprot ID   -
Organism   Aquitalea denitrificans strain 5YN1-3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3718719..3730431
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GSR16_RS17110 yacG 3720004..3720201 (-) 198 WP_159879523.1 DNA gyrase inhibitor YacG -
  GSR16_RS17115 zapD 3720207..3720965 (-) 759 WP_159879525.1 cell division protein ZapD -
  GSR16_RS17120 coaE 3721005..3721616 (-) 612 WP_159879527.1 dephospho-CoA kinase -
  GSR16_RS17125 pilD 3721606..3722475 (-) 870 WP_159879529.1 prepilin peptidase Machinery gene
  GSR16_RS17130 pilC 3722478..3723698 (-) 1221 WP_159879531.1 type II secretion system F family protein Machinery gene
  GSR16_RS17135 pilF 3723719..3725431 (-) 1713 WP_159879533.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GSR16_RS17140 - 3725615..3726697 (-) 1083 WP_159879535.1 porin -
  GSR16_RS17145 - 3727039..3728430 (+) 1392 WP_159879538.1 cryptochrome/photolyase family protein -
  GSR16_RS17150 comE 3728489..3728851 (-) 363 WP_159879540.1 ComEA family DNA-binding protein Machinery gene
  GSR16_RS17155 - 3728899..3729720 (-) 822 WP_159879542.1 cytochrome C assembly family protein -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 62014.21 Da        Isoelectric Point: 5.4148

>NTDB_id=411798 GSR16_RS17135 WP_159879533.1 3723719..3725431(-) (pilF) [Aquitalea denitrificans strain 5YN1-3]
MEASAGISGLGRALVQNNALLLQDAEAIQRQSQASSISFVEQLIASKKMSAGDVAAFASRVFGYPLLDMGSIDPSQLPKG
LVDDELIGSRRLVPLFKRGNKLFIGTSDPTQTSAFHAITFKTGLTVEVVVVEDDKLSKVIGKYTEDASAAIKALENEDFG
DLEFVDPDAQTPHSETNQPDVDDAPVVKFIHKVLLDAINGGASDVHFEPYEKFYRIRYRVDGQLKEIAQPPLLLKEKIAS
RIKVISRLDISEKRVPQDGRLKLVLSKSHSIDFRVSTLPTLYGEKIVMRILDSSAASLNIEQLGFEPEQKQMLLDAIGRP
YGMVLVTGPTGSGKTVSLYTCLNILNKPDINISTAEDPVEINLPGINQVNVNEKAGLTFSSALKAFLRQDPDVIMVGEIR
DFETADISIKAAQTGHMVFSTLHTNNAPATLTRLLNMGVAPFNVASSVLLIMAQRLARRLCSHCKAPVDIPEPALLRAGF
RAEELDGSWKPYGPVGCDECRGSGYKGRTGIYEVMPISDAMTRLIMQNGTAIDINDLALQEGMIDLRHAGLLKVKKGLTS
LAEIEAVTND

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=411798 GSR16_RS17135 WP_159879533.1 3723719..3725431(-) (pilF) [Aquitalea denitrificans strain 5YN1-3]
ATGGAAGCTTCTGCGGGGATATCTGGCCTTGGGCGCGCGCTGGTTCAAAACAATGCACTGCTGCTGCAGGACGCCGAGGC
TATCCAGCGTCAGTCCCAGGCATCCAGTATCAGTTTTGTCGAGCAGCTGATTGCCAGCAAGAAAATGTCTGCAGGAGATG
TGGCGGCGTTTGCTTCGCGTGTGTTTGGCTATCCCTTGCTGGACATGGGGTCGATTGATCCTTCCCAGCTACCCAAGGGC
TTGGTGGATGACGAGCTGATTGGCAGCCGCCGCCTAGTCCCCTTGTTCAAGCGTGGCAACAAGCTGTTTATCGGTACTTC
TGATCCGACGCAGACCTCGGCCTTTCACGCGATTACCTTCAAGACCGGGTTGACGGTTGAGGTCGTGGTTGTCGAGGATG
ACAAGCTTTCAAAAGTCATCGGCAAATATACGGAAGATGCTTCTGCAGCCATCAAGGCGCTAGAAAACGAAGATTTTGGT
GATCTGGAGTTTGTCGATCCGGATGCGCAGACACCGCATAGTGAAACCAACCAGCCGGATGTGGATGACGCTCCTGTCGT
CAAGTTTATTCACAAGGTGTTGCTGGATGCCATCAACGGTGGTGCCTCCGACGTACACTTCGAACCGTATGAGAAGTTTT
ACCGTATCCGTTACCGGGTGGATGGCCAGCTCAAGGAAATTGCCCAGCCGCCATTGCTGCTGAAGGAAAAGATTGCCTCC
CGGATCAAGGTTATTTCCCGGCTGGATATTTCTGAAAAACGGGTACCACAAGACGGTCGTCTGAAGCTGGTATTGTCCAA
GTCCCATTCCATCGACTTCCGCGTCAGTACCTTGCCGACCTTGTACGGCGAGAAGATCGTGATGCGTATTCTTGATTCCT
CGGCGGCATCACTGAATATCGAACAGCTGGGCTTCGAGCCGGAACAGAAACAGATGCTGCTGGATGCTATCGGCCGGCCA
TATGGAATGGTGCTGGTGACCGGGCCAACCGGTAGTGGTAAAACGGTATCCTTGTATACCTGCTTGAATATCCTGAACAA
GCCGGATATCAATATCTCCACGGCGGAAGATCCGGTCGAAATTAATTTGCCGGGCATTAACCAGGTCAACGTTAACGAGA
AAGCCGGTCTGACGTTTTCGTCCGCCCTGAAGGCTTTCTTGCGTCAGGATCCTGATGTCATCATGGTGGGTGAAATTCGC
GACTTCGAAACTGCCGATATTTCCATCAAGGCAGCTCAGACCGGGCATATGGTTTTTTCCACCCTTCATACCAACAATGC
GCCCGCCACCCTGACCCGTTTGCTCAATATGGGGGTGGCACCATTCAATGTCGCCAGCTCGGTACTGTTGATCATGGCCC
AGCGTCTGGCGCGCCGCTTGTGCAGTCATTGTAAGGCACCCGTCGATATTCCCGAGCCCGCCTTGCTGCGCGCTGGTTTC
CGGGCCGAGGAGCTGGATGGCAGCTGGAAGCCATACGGCCCGGTTGGCTGTGATGAGTGCCGTGGATCCGGCTATAAAGG
ACGTACCGGTATTTATGAAGTCATGCCGATCAGTGATGCCATGACGCGTCTGATCATGCAAAATGGCACTGCAATTGATA
TCAATGATCTGGCCCTGCAAGAAGGGATGATCGATCTGCGACATGCTGGTCTGCTGAAGGTCAAGAAAGGGCTGACTTCC
TTGGCTGAAATCGAGGCAGTGACCAACGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(47-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

57.319

99.474

0.57
  pilB Acinetobacter baumannii D1279779

54.059

100

0.549
  pilB Acinetobacter baylyi ADP1

53.982

99.123

0.535
  pilB Legionella pneumophila strain ERS1305867

52.734

99.474

0.525
  pilB Vibrio cholerae strain A1552

50.183

95.789

0.481
  pilB Vibrio parahaemolyticus RIMD 2210633

47.163

98.947

0.467
  pilB Vibrio campbellii strain DS40M4

46.195

99.123

0.458
  pilF Thermus thermophilus HB27

38.095

99.474

0.379
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.083

95.614

0.374

Multiple sequence alignment