Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   GSF13_RS10045 Genome accession   NZ_CP047240
Coordinates   1161635..1162864 (-) Length   409 a.a.
NCBI ID   WP_211868085.1    Uniprot ID   -
Organism   Pseudoalteromonas sp. M8     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1156635..1167864
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GSF13_RS10025 (GSF13_10045) rlmM 1156903..1157988 (+) 1086 WP_010607344.1 23S rRNA (cytidine(2498)-2'-O)-methyltransferase RlmM -
  GSF13_RS10030 (GSF13_10050) pilB 1158197..1159873 (-) 1677 WP_010607343.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GSF13_RS10035 (GSF13_10055) coaE 1159983..1160612 (-) 630 WP_211868081.1 dephospho-CoA kinase -
  GSF13_RS10040 (GSF13_10060) pilD 1160622..1161500 (-) 879 WP_211868083.1 A24 family peptidase Machinery gene
  GSF13_RS10045 (GSF13_10065) pilC 1161635..1162864 (-) 1230 WP_211868085.1 type II secretion system F family protein Machinery gene
  GSF13_RS10050 (GSF13_10070) - 1162926..1163384 (-) 459 WP_130126778.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  GSF13_RS10055 (GSF13_10075) nadC 1164042..1164884 (-) 843 WP_211868994.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GSF13_RS10060 (GSF13_10080) ampD 1165428..1165970 (+) 543 WP_010607337.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GSF13_RS10065 (GSF13_10085) ampE 1165977..1166828 (+) 852 WP_211868087.1 beta-lactamase regulator AmpE -

Sequence

Protein


Download         Length: 409 a.a.        Molecular weight: 44854.90 Da        Isoelectric Point: 9.8055

>NTDB_id=411769 GSF13_RS10045 WP_211868085.1 1161635..1162864(-) (pilC) [Pseudoalteromonas sp. M8]
MKSKKNESNLDTFIWAGFNSRGKKLNGEMTGQSVALVKAQLRKQGITPSKVKRKPKPLFGFSGTQKIKTVDIATTTRQIA
TMLMAGVSLVQTLDMLARGIKNKSLSKLISHICDEVKAGQPLAKSLRAYPRYFDELYCDLVHSGEQSGALDTIFDRIALY
KEKTEALKSKIKKALFYPIAVIVVALIVTSILLIFVVPQFQDIFAGFGAELPAFTLMVIAISEFMQEYWWVFVLGIGLFG
YTYKEMLIRSAAVKHFNDRMVLRIPVIGEILKKAAVARYARTLSTTFAAGVPLGDALDSAAGASGNIIYKNAIKEIKAEV
NSGNQMHWAMHNTKVFPDMVVQMVSIGEESGSLDGMLAKVANIYEQEVDDAVDGLSSLLEPLIMVVLGVLIGGLIIAMYL
PIFQLGSVI

Nucleotide


Download         Length: 1230 bp        

>NTDB_id=411769 GSF13_RS10045 WP_211868085.1 1161635..1162864(-) (pilC) [Pseudoalteromonas sp. M8]
ATGAAATCAAAAAAAAACGAATCAAACCTTGATACCTTTATTTGGGCTGGCTTTAATAGTCGAGGCAAAAAGCTCAATGG
CGAAATGACTGGACAGAGCGTGGCTCTAGTTAAAGCCCAATTACGTAAACAAGGAATCACACCTTCCAAAGTTAAACGCA
AGCCTAAACCTCTCTTTGGCTTTTCTGGTACTCAAAAAATTAAAACTGTTGATATTGCAACGACAACTCGTCAAATTGCT
ACAATGTTAATGGCGGGTGTTTCTTTAGTACAAACATTGGATATGCTTGCTCGTGGCATAAAAAATAAAAGCTTATCTAA
ATTAATCAGCCATATTTGTGATGAAGTAAAGGCAGGCCAGCCACTTGCTAAATCACTAAGAGCTTACCCTAGATATTTTG
ATGAACTTTATTGTGATCTTGTCCATTCAGGTGAACAGTCTGGTGCACTTGATACCATATTTGACCGCATCGCACTCTAT
AAAGAAAAAACAGAAGCGTTAAAGTCTAAAATCAAAAAGGCGCTGTTTTATCCAATTGCTGTGATTGTTGTCGCGCTAAT
TGTTACTTCAATATTATTGATATTTGTTGTACCTCAATTTCAAGATATCTTTGCTGGTTTTGGTGCTGAACTCCCTGCCT
TTACCCTAATGGTTATCGCGATTTCCGAATTTATGCAGGAATACTGGTGGGTATTTGTGCTTGGTATTGGCCTTTTTGGT
TATACCTATAAAGAAATGCTTATTCGCAGTGCTGCGGTAAAGCATTTTAACGATCGTATGGTTTTACGTATTCCAGTGAT
TGGCGAGATCCTGAAAAAAGCGGCGGTGGCGCGTTATGCTCGTACGCTTTCTACCACCTTTGCAGCGGGTGTTCCCCTTG
GCGATGCACTAGATTCTGCCGCAGGTGCGTCGGGCAATATTATTTACAAAAACGCAATTAAAGAAATAAAAGCAGAGGTT
AACTCGGGTAACCAAATGCACTGGGCAATGCATAACACCAAAGTCTTTCCAGATATGGTAGTGCAAATGGTGTCTATTGG
TGAAGAATCGGGTTCTTTAGACGGTATGCTGGCAAAGGTCGCTAACATTTATGAGCAAGAAGTCGATGATGCGGTGGATG
GCCTATCTAGCCTATTAGAACCATTAATTATGGTGGTACTCGGCGTACTGATCGGTGGCCTGATTATCGCCATGTACTTA
CCGATATTCCAACTAGGTTCAGTTATTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(280-401)

Type II secretion system (T2SS), protein F (T2SSF)

(76-198)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

59.698

97.066

0.579
  pilC Legionella pneumophila strain ERS1305867

54.568

99.022

0.54
  pilC Acinetobacter baylyi ADP1

51.345

100

0.513
  pilC Acinetobacter baumannii D1279779

51.605

99.022

0.511
  pilC Vibrio campbellii strain DS40M4

43.873

99.756

0.438
  pilC Vibrio cholerae strain A1552

44.279

98.289

0.435
  pilG Neisseria meningitidis 44/76-A

43.467

97.311

0.423
  pilG Neisseria gonorrhoeae MS11

43.467

97.311

0.423

Multiple sequence alignment