Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GSF13_RS10030 Genome accession   NZ_CP047240
Coordinates   1158197..1159873 (-) Length   558 a.a.
NCBI ID   WP_010607343.1    Uniprot ID   A0A2K9JV15
Organism   Pseudoalteromonas sp. M8     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1153197..1164873
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GSF13_RS10005 (GSF13_10025) kdsA 1154048..1154902 (+) 855 WP_010370475.1 3-deoxy-8-phosphooctulonate synthase -
  GSF13_RS10010 (GSF13_10030) - 1154988..1155893 (+) 906 WP_128725963.1 transcriptional regulator GcvA -
  GSF13_RS10015 (GSF13_10035) - 1155890..1156528 (+) 639 WP_211868079.1 alpha/beta family hydrolase -
  GSF13_RS10020 (GSF13_10040) - 1156521..1156889 (+) 369 WP_017217933.1 DUF423 domain-containing protein -
  GSF13_RS10025 (GSF13_10045) rlmM 1156903..1157988 (+) 1086 WP_010607344.1 23S rRNA (cytidine(2498)-2'-O)-methyltransferase RlmM -
  GSF13_RS10030 (GSF13_10050) pilB 1158197..1159873 (-) 1677 WP_010607343.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GSF13_RS10035 (GSF13_10055) coaE 1159983..1160612 (-) 630 WP_211868081.1 dephospho-CoA kinase -
  GSF13_RS10040 (GSF13_10060) pilD 1160622..1161500 (-) 879 WP_211868083.1 A24 family peptidase Machinery gene
  GSF13_RS10045 (GSF13_10065) pilC 1161635..1162864 (-) 1230 WP_211868085.1 type II secretion system F family protein Machinery gene
  GSF13_RS10050 (GSF13_10070) - 1162926..1163384 (-) 459 WP_130126778.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -

Sequence

Protein


Download         Length: 558 a.a.        Molecular weight: 62014.26 Da        Isoelectric Point: 5.5806

>NTDB_id=411767 GSF13_RS10030 WP_010607343.1 1158197..1159873(-) (pilB) [Pseudoalteromonas sp. M8]
MEHHSPLLRKFITLGRVTAEQIKSRQSEANTTAELICLSSGMSSKELAEECLDLFKVPFFNLDHFNVSEIPPALVKEKLI
RKHHILPLVQKGRKLYVAASDPTDFGAFENFEFSTGLQCEVLVADYKKLEAKIDQLFDATGGLSISEEEFKEFADLEVDD
EPKQQNTNEDKDDAPIIVYINKILMDAIKKGASDLHFEPYEKKYRVRFRIDGLLHEMASPPNTLATRLAARIKVMAHLDI
AEKRKPQDGRIKLKISERKSIDFRVSTLPTMWGEKIVMRILDSSSAMLGIDVLGYEPEQKQLYLDALEQPQGMILVTGPT
GSGKTVSLYTGLNILNQPERNISTAEDPVEINLEGINQVQINTKADMTFANALRAFLRQDPDVVMVGEIRDLETAEISIK
AAQTGHLVLSTLHTNSAPETLTRLLNMGVPAYNVASSVSLIIAQRLARRLCPKCKTPETLPEEALLGQGFTAEQIKEITL
FAPKGCENCTDGYKGRVGIYEVVKITPELSHLIMEGGNSLEIAEKAEQLGFDNLRKSGLKKAAAGVTSLTEINRVTSY

Nucleotide


Download         Length: 1677 bp        

>NTDB_id=411767 GSF13_RS10030 WP_010607343.1 1158197..1159873(-) (pilB) [Pseudoalteromonas sp. M8]
ATGGAACATCATTCCCCGCTACTGCGTAAATTTATTACCTTAGGGCGAGTTACTGCTGAGCAAATAAAGTCTCGCCAGTC
AGAAGCAAACACCACCGCCGAGTTGATTTGCCTAAGTTCAGGAATGAGCTCCAAAGAGCTAGCTGAAGAATGCCTAGACT
TATTTAAGGTGCCCTTTTTTAATCTTGATCACTTTAACGTTAGTGAAATTCCACCGGCGCTAGTCAAAGAAAAGCTGATC
CGTAAGCACCATATTTTACCGCTGGTACAAAAAGGTAGAAAACTCTATGTAGCGGCCTCCGACCCTACTGATTTCGGCGC
ATTTGAAAACTTTGAGTTTAGTACTGGATTACAGTGTGAAGTCTTAGTCGCCGACTACAAAAAGCTCGAAGCAAAGATTG
ATCAGCTGTTTGATGCAACGGGCGGTCTCTCGATCTCTGAAGAAGAATTTAAAGAATTTGCCGACTTAGAAGTTGATGAC
GAGCCTAAACAGCAAAACACCAACGAAGATAAAGACGATGCGCCAATCATCGTTTATATCAATAAAATCTTAATGGACGC
GATAAAAAAAGGCGCGTCGGATCTGCACTTTGAGCCCTACGAAAAAAAATACCGCGTGCGTTTTCGTATCGATGGCCTGC
TGCATGAGATGGCAAGTCCACCAAACACACTGGCTACTCGCCTCGCCGCCCGTATCAAAGTGATGGCGCACTTAGACATA
GCGGAAAAACGTAAACCGCAAGATGGCCGCATTAAATTAAAGATTTCCGAGCGCAAAAGTATCGACTTTCGTGTGAGTAC
GCTGCCTACCATGTGGGGCGAGAAAATCGTAATGCGTATTCTAGATTCGTCCAGCGCCATGCTCGGCATTGACGTGCTAG
GTTATGAACCTGAGCAAAAGCAGCTTTACCTCGACGCACTAGAGCAGCCTCAAGGCATGATTTTGGTAACCGGCCCAACG
GGTTCCGGTAAAACCGTGTCACTTTACACTGGCTTGAATATTCTTAATCAGCCAGAGCGCAATATCAGCACAGCGGAAGA
CCCTGTCGAAATAAACCTTGAGGGTATTAACCAAGTACAAATCAATACCAAAGCGGATATGACCTTCGCCAATGCCTTGA
GAGCATTTTTGCGTCAGGATCCGGATGTCGTGATGGTTGGTGAGATCCGTGACCTTGAAACCGCTGAGATCTCGATAAAA
GCCGCCCAAACCGGTCACTTAGTATTGAGTACGCTGCACACCAATTCTGCGCCTGAAACGCTCACCCGTCTATTAAACAT
GGGCGTGCCGGCATACAACGTAGCAAGCTCGGTGAGTTTGATTATTGCTCAGCGTCTCGCAAGACGCCTTTGCCCTAAGT
GTAAAACACCTGAAACGCTGCCGGAAGAAGCCTTGCTAGGGCAAGGCTTTACAGCCGAGCAAATTAAAGAGATTACCCTT
TTTGCGCCTAAAGGCTGTGAAAACTGCACCGATGGTTACAAAGGCCGTGTGGGTATATATGAAGTGGTAAAAATCACGCC
AGAGCTTTCACACCTGATCATGGAAGGCGGCAATTCTCTTGAGATTGCCGAAAAAGCAGAGCAGTTAGGTTTTGATAACT
TACGTAAGTCTGGCCTGAAAAAAGCCGCCGCAGGGGTGACATCGCTTACAGAAATCAACCGCGTAACAAGCTATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-140)

Type II/IV secretion system protein (T2SSE)

(182-449)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2K9JV15

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.895

100

0.591
  pilB Acinetobacter baylyi ADP1

57.27

100

0.579
  pilB Vibrio cholerae strain A1552

53.455

98.566

0.527
  pilB Legionella pneumophila strain ERS1305867

54.894

93.369

0.513
  pilB Vibrio parahaemolyticus RIMD 2210633

53.977

94.624

0.511
  pilB Vibrio campbellii strain DS40M4

53.497

94.803

0.507
  pilF Neisseria gonorrhoeae MS11

47.133

100

0.471
  pilB/pilB1 Synechocystis sp. PCC 6803

36.013

100

0.401
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.429

94.086

0.371
  pilF Thermus thermophilus HB27

36.396

99.462

0.362

Multiple sequence alignment