Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   GSF04_RS09190 Genome accession   NZ_CP047238
Coordinates   953459..954694 (-) Length   411 a.a.
NCBI ID   WP_211859334.1    Uniprot ID   -
Organism   Pseudoalteromonas sp. A22     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 948459..959694
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GSF04_RS09175 (GSF04_09215) pilB 950046..951722 (-) 1677 WP_130164712.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GSF04_RS09180 (GSF04_09220) coaE 951818..952447 (-) 630 WP_211859332.1 dephospho-CoA kinase -
  GSF04_RS09185 (GSF04_09225) pilD 952457..953335 (-) 879 WP_211859333.1 A24 family peptidase Machinery gene
  GSF04_RS09190 (GSF04_09230) pilC 953459..954694 (-) 1236 WP_211859334.1 type II secretion system F family protein Machinery gene
  GSF04_RS09195 (GSF04_09235) - 954749..955186 (-) 438 WP_211859335.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  GSF04_RS23515 (GSF04_09240) - 955658..955867 (-) 210 WP_349251739.1 hypothetical protein -
  GSF04_RS09200 (GSF04_09245) nadC 956120..956962 (-) 843 WP_211859922.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GSF04_RS09205 (GSF04_09250) ampD 957744..958286 (+) 543 WP_211859336.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GSF04_RS09210 (GSF04_09255) ampE 958293..959144 (+) 852 WP_010370512.1 beta-lactamase regulator AmpE -

Sequence

Protein


Download         Length: 411 a.a.        Molecular weight: 44863.72 Da        Isoelectric Point: 9.6364

>NTDB_id=411739 GSF04_RS09190 WP_211859334.1 953459..954694(-) (pilC) [Pseudoalteromonas sp. A22]
MNKKSIESSNTLDTFMWVGVNNRGKRLEGEITGSSVALVKAQLRKQGITPSKVKRKPKPLFGLSGAQKITPQDIATVTRQ
IATMLTAGVSLVQTLEMLARGSKNKSLSKLIGHVGDEVKAGQPLAKALRAYPRYFDELYCDLVHSGEQSGALDTIFDRIA
LYKEKTEALKSKIKKALFYPIAVIVVALIVTSILLIFVVPQFQDIFNGFGAELPAFTLMVIGFSEFLQEYWWVFLLGLGL
FGYTYKEMLIRSPAVKHFNDRMILKLPVVGEILKKAAVARYARTLSTTFAAGVPLGDALDSAAGASGNIIYKNAIKEIKA
EVNSGNQMHWAMHNTKVFPDMVVQMVSIGEESGSLDGMLAKVANIYEQEVDDAVDGLSSLLEPLIMVVLGVLIGGLIIAM
YLPIFQLGSVV

Nucleotide


Download         Length: 1236 bp        

>NTDB_id=411739 GSF04_RS09190 WP_211859334.1 953459..954694(-) (pilC) [Pseudoalteromonas sp. A22]
ATGAATAAGAAATCTATAGAATCTAGCAATACTTTAGACACCTTTATGTGGGTCGGAGTAAATAATAGAGGTAAACGCCT
CGAAGGCGAAATAACAGGCTCGAGTGTCGCTTTAGTCAAAGCGCAGCTAAGAAAACAGGGCATTACTCCTTCCAAAGTCA
AGCGTAAACCTAAACCACTGTTTGGTTTGTCTGGTGCGCAAAAAATCACTCCTCAAGATATTGCAACGGTCACAAGACAA
ATTGCCACTATGTTGACTGCTGGTGTATCTCTAGTACAGACTCTTGAGATGCTGGCCAGAGGCAGCAAAAATAAATCATT
GTCTAAACTCATAGGTCATGTGGGCGATGAAGTGAAGGCAGGTCAGCCTTTAGCGAAAGCATTACGTGCTTACCCCAGAT
ATTTTGATGAACTTTATTGCGACCTTGTTCATTCAGGTGAACAGTCTGGCGCGCTAGATACCATATTTGACCGCATAGCG
CTCTATAAAGAAAAAACCGAAGCGCTGAAATCTAAAATTAAGAAAGCATTGTTTTACCCGATAGCTGTCATTGTTGTCGC
GTTAATTGTTACCTCTATATTATTAATTTTTGTCGTACCTCAATTTCAAGACATATTTAACGGTTTTGGAGCTGAGTTAC
CTGCATTTACGCTTATGGTTATTGGTTTCTCTGAGTTTCTACAAGAGTACTGGTGGGTGTTTTTGCTCGGACTTGGCCTA
TTTGGTTATACCTATAAAGAAATGCTGATTCGCAGTCCTGCTGTAAAACACTTTAACGACAGAATGATACTTAAACTTCC
CGTTGTCGGTGAAATTTTGAAAAAAGCGGCGGTGGCGCGTTATGCTCGTACGCTTTCGACTACCTTTGCAGCGGGTGTTC
CCCTTGGCGATGCACTCGATTCTGCTGCAGGTGCATCAGGCAATATTATTTACAAAAACGCGATTAAAGAAATAAAAGCA
GAGGTTAACTCGGGTAACCAAATGCACTGGGCAATGCATAACACCAAAGTCTTCCCTGATATGGTGGTGCAAATGGTGTC
TATCGGTGAAGAATCGGGCTCGCTAGATGGTATGCTGGCAAAAGTTGCAAACATTTATGAGCAAGAAGTCGATGATGCGG
TGGATGGCCTATCTAGCCTATTAGAACCATTAATTATGGTGGTACTCGGCGTACTGATCGGTGGCCTTATCATTGCTATG
TACTTGCCTATATTCCAATTAGGCTCAGTGGTGTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(78-200)

Type II secretion system (T2SS), protein F (T2SSF)

(282-403)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

59.124

100

0.591
  pilC Legionella pneumophila strain ERS1305867

54.568

98.54

0.538
  pilC Acinetobacter baumannii D1279779

51.351

99.027

0.509
  pilC Acinetobacter baylyi ADP1

51.358

98.54

0.506
  pilC Vibrio campbellii strain DS40M4

43.781

97.81

0.428
  pilC Vibrio cholerae strain A1552

43.284

97.81

0.423
  pilG Neisseria gonorrhoeae MS11

43.216

96.837

0.418
  pilG Neisseria meningitidis 44/76-A

43.216

96.837

0.418

Multiple sequence alignment