Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GSF04_RS09175 Genome accession   NZ_CP047238
Coordinates   950046..951722 (-) Length   558 a.a.
NCBI ID   WP_130164712.1    Uniprot ID   -
Organism   Pseudoalteromonas sp. A22     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 945046..956722
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GSF04_RS09150 (GSF04_09190) kdsA 945134..945988 (+) 855 WP_010370475.1 3-deoxy-8-phosphooctulonate synthase -
  GSF04_RS09155 (GSF04_09195) - 946074..946979 (+) 906 WP_088531408.1 transcriptional regulator GcvA -
  GSF04_RS09160 (GSF04_09200) - 946976..947614 (+) 639 WP_045989837.1 alpha/beta family hydrolase -
  GSF04_RS09165 (GSF04_09205) - 947607..947975 (+) 369 WP_095728687.1 DUF423 domain-containing protein -
  GSF04_RS09170 (GSF04_09210) rlmM 947989..949074 (+) 1086 WP_088531405.1 23S rRNA (cytidine(2498)-2'-O)-methyltransferase RlmM -
  GSF04_RS09175 (GSF04_09215) pilB 950046..951722 (-) 1677 WP_130164712.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GSF04_RS09180 (GSF04_09220) coaE 951818..952447 (-) 630 WP_211859332.1 dephospho-CoA kinase -
  GSF04_RS09185 (GSF04_09225) pilD 952457..953335 (-) 879 WP_211859333.1 A24 family peptidase Machinery gene
  GSF04_RS09190 (GSF04_09230) pilC 953459..954694 (-) 1236 WP_211859334.1 type II secretion system F family protein Machinery gene
  GSF04_RS09195 (GSF04_09235) - 954749..955186 (-) 438 WP_211859335.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  GSF04_RS23515 (GSF04_09240) - 955658..955867 (-) 210 WP_349251739.1 hypothetical protein -

Sequence

Protein


Download         Length: 558 a.a.        Molecular weight: 61997.27 Da        Isoelectric Point: 5.5806

>NTDB_id=411737 GSF04_RS09175 WP_130164712.1 950046..951722(-) (pilB) [Pseudoalteromonas sp. A22]
MEHHSPLLRKFITLGRVTAEQIKSRQPEANTTAELICLSSGMSSKELAEECLDLFKVPFFNLDHFNVSEIPPALVKEKLI
RKHHILPLVQKGRKLYVAASDPTDFGAFENFEFSTGLQCEVLVADYKKLEAKIDQLFDATGGLSISEEEFKEFADLEVDD
EPKQQNTNEDKDDAPIIVYINKILMDAIKKGASDLHFEPYEKKYRVRFRIDGLLHEMASPPNTLATRLAARIKVMAHLDI
AEKRKPQDGRIKLKISERKSIDFRVSTLPTMWGEKIVMRILDSSSAMLGIDVLGYEPEQKQLYLDALEQPQGMILVTGPT
GSGKTVSLYTGLNILNQPERNISTAEDPVEINLEGINQVQINTKADMTFANALRAFLRQDPDVVMVGEIRDLETAEISIK
AAQTGHLVLSTLHTNSAPETLTRLLNMGVPAYNVASSVSLIIAQRLARRLCPKCKTPETLPEEALLGQGFTAEQIKEITL
FAPKGCESCTDGYKGRVGIYEVVKITPELSHLIMEGGNSLEIAEKAEQLGFDNLRKSGLKKAAAGVTSLTEINRVTSY

Nucleotide


Download         Length: 1677 bp        

>NTDB_id=411737 GSF04_RS09175 WP_130164712.1 950046..951722(-) (pilB) [Pseudoalteromonas sp. A22]
ATGGAACATCATTCCCCGCTACTGCGTAAATTTATTACCTTAGGGCGAGTCACTGCTGAGCAAATAAAGTCTCGCCAGCC
AGAAGCAAACACCACCGCCGAGTTAATTTGCCTAAGTTCAGGAATGAGCTCCAAAGAGCTAGCTGAAGAATGCCTAGACT
TATTCAAGGTGCCCTTTTTTAATCTTGATCACTTTAACGTTAGTGAAATTCCACCAGCGCTAGTCAAAGAAAAACTGATC
CGTAAGCACCATATTTTACCGCTGGTACAAAAAGGTAGAAAACTCTACGTTGCGGCTTCTGATCCTACTGACTTTGGCGC
ATTTGAAAACTTTGAGTTTAGTACTGGATTACAGTGTGAAGTACTGGTAGCCGACTACAAAAAACTCGAAGCAAAGATTG
ATCAGCTGTTTGATGCAACGGGCGGCCTCTCGATCTCTGAAGAAGAATTTAAAGAATTTGCCGACTTAGAAGTTGATGAC
GAGCCTAAACAGCAAAACACCAACGAAGACAAAGACGATGCGCCAATCATCGTTTATATCAATAAAATCTTAATGGACGC
GATAAAAAAAGGCGCGTCGGATCTACACTTTGAGCCCTACGAGAAGAAATATCGTGTGCGCTTTCGTATCGATGGCCTGC
TGCATGAGATGGCAAGCCCACCAAACACATTAGCTACTCGTCTCGCCGCCCGTATCAAAGTGATGGCGCATTTAGACATA
GCAGAAAAACGCAAACCGCAAGATGGCCGCATTAAATTAAAGATCTCCGAGCGCAAAAGTATCGATTTTCGTGTAAGTAC
ACTGCCTACCATGTGGGGCGAGAAAATCGTAATGCGTATTCTAGATTCGTCCAGCGCCATGCTCGGCATTGACGTGCTAG
GTTATGAGCCTGAGCAAAAACAGCTTTACCTCGACGCACTAGAACAGCCTCAGGGCATGATTTTGGTAACCGGTCCAACG
GGTTCCGGTAAAACCGTGTCACTTTACACTGGCTTGAATATTCTTAATCAGCCAGAGCGCAATATCAGCACGGCGGAAGA
CCCTGTCGAAATAAACCTTGAGGGTATTAACCAAGTACAAATCAATACCAAAGCGGATATGACTTTCGCCAATGCCTTGA
GAGCATTCTTACGTCAGGATCCGGATGTGGTGATGGTTGGTGAGATCCGTGACCTTGAAACCGCTGAGATCTCAATAAAA
GCCGCCCAAACCGGTCACTTAGTATTGAGTACGCTGCACACCAATTCTGCGCCTGAAACGCTCACCCGTCTATTAAACAT
GGGCGTGCCGGCGTACAACGTAGCAAGCTCGGTGAGTCTGATTATTGCCCAGCGTCTCGCAAGACGCCTTTGCCCTAAGT
GTAAAACACCCGAAACACTGCCGGAAGAAGCCTTGCTAGGGCAAGGCTTTACTGCCGAGCAAATTAAAGAGATTACCCTT
TTTGCACCTAAAGGCTGTGAAAGCTGCACCGATGGTTACAAAGGCCGTGTGGGTATTTATGAAGTGGTAAAAATCACGCC
AGAGCTTTCGCACCTGATCATGGAAGGGGGAAACTCTCTTGAGATTGCCGAAAAAGCAGAGCAGTTAGGTTTTGATAACT
TACGTAAGTCTGGCCTGAAAAAAGCCGCCGCAGGGGTGACATCGCTTACAGAAATCAACCGCGTAACGAGTTATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-140)

Type II/IV secretion system protein (T2SSE)

(182-449)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.895

100

0.591
  pilB Acinetobacter baylyi ADP1

57.27

100

0.579
  pilB Vibrio cholerae strain A1552

53.455

98.566

0.527
  pilB Vibrio parahaemolyticus RIMD 2210633

53.005

98.387

0.522
  pilB Vibrio campbellii strain DS40M4

52.545

98.566

0.518
  pilB Legionella pneumophila strain ERS1305867

54.894

93.369

0.513
  pilF Neisseria gonorrhoeae MS11

47.133

100

0.471
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.429

94.086

0.371
  pilF Thermus thermophilus HB27

36.396

99.462

0.362

Multiple sequence alignment