Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   GNX18_RS00645 Genome accession   NZ_CP046948
Coordinates   131480..132694 (-) Length   404 a.a.
NCBI ID   WP_166214792.1    Uniprot ID   A0A6G8DA54
Organism   Microbulbifer sp. SH-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 129969..145038 131480..132694 within 0


Gene organization within MGE regions


Location: 129969..145038
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GNX18_RS00630 (GNX18_00625) - 129969..130262 (-) 294 WP_166214788.1 HigA family addiction module antitoxin -
  GNX18_RS19490 (GNX18_00630) - 130262..130447 (-) 186 WP_256367352.1 type II toxin-antitoxin system RelE/ParE family toxin -
  GNX18_RS00640 (GNX18_00635) - 130582..131466 (-) 885 WP_166214790.1 A24 family peptidase -
  GNX18_RS00645 (GNX18_00640) pilC 131480..132694 (-) 1215 WP_166214792.1 type II secretion system F family protein Machinery gene
  GNX18_RS00650 (GNX18_00645) pilB 132746..134455 (-) 1710 WP_166214794.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GNX18_RS00655 (GNX18_00650) comP 134947..135387 (+) 441 WP_205828022.1 pilin Machinery gene
  GNX18_RS00660 (GNX18_00655) - 135438..137186 (+) 1749 WP_166214798.1 DCC1-like thiol-disulfide oxidoreductase family protein -
  GNX18_RS00665 (GNX18_00660) - 137279..137569 (-) 291 WP_166214799.1 DUF86 domain-containing protein -
  GNX18_RS00670 (GNX18_00665) - 137741..137914 (+) 174 WP_166214801.1 hypothetical protein -
  GNX18_RS00675 (GNX18_00670) - 138249..138422 (+) 174 WP_166214801.1 hypothetical protein -
  GNX18_RS00680 (GNX18_00675) - 138447..138677 (+) 231 WP_166214803.1 hypothetical protein -
  GNX18_RS00685 (GNX18_00680) - 138780..139055 (+) 276 WP_166214805.1 hypothetical protein -
  GNX18_RS00690 (GNX18_00685) - 139423..140103 (+) 681 WP_166214807.1 hypothetical protein -
  GNX18_RS19500 - 140617..140847 (-) 231 WP_240914129.1 hypothetical protein -
  GNX18_RS00700 (GNX18_00695) - 140844..141173 (-) 330 WP_166214809.1 winged helix-turn-helix domain-containing protein -
  GNX18_RS00705 (GNX18_00700) - 141487..141774 (-) 288 WP_166214811.1 type II toxin-antitoxin system RelE/ParE family toxin -
  GNX18_RS00710 (GNX18_00705) - 141778..142008 (-) 231 WP_166214813.1 CopG family transcriptional regulator -
  GNX18_RS00715 (GNX18_00710) - 142180..143535 (-) 1356 WP_166214815.1 Fic family protein -
  GNX18_RS00720 (GNX18_00715) - 143771..144184 (-) 414 WP_166214817.1 DUF86 domain-containing protein -
  GNX18_RS00725 (GNX18_00720) - 144181..144618 (-) 438 WP_138235878.1 nucleotidyltransferase domain-containing protein -
  GNX18_RS00730 (GNX18_00725) - 144793..145038 (+) 246 WP_138235879.1 YdcH family protein -

Sequence

Protein


Download         Length: 404 a.a.        Molecular weight: 43531.26 Da        Isoelectric Point: 9.4214

>NTDB_id=409592 GNX18_RS00645 WP_166214792.1 131480..132694(-) (pilC) [Microbulbifer sp. SH-1]
MAKAAVAVAYIYKGVDAKGNKVEGEINSTNPALIKAQLRRQGIIANKVQKKATPLFGGNKKIKPGDIAIFTRQMATMMKA
GVPLVQSFEIVADGLENAGMKELIFKIRDDVASGTAFADSLRRHSLYFDDLFCNLVASGEQSGALETMLDRIATYKEKTE
SLKKKIKKAMTYPIAVVVVAVVVTAILLVKVVPQFAETFSSFGADLPAFTLFVLGISEWMQANWFYAFLGVVVGIGGTIE
VKKRNKNVANFFDKLMLKLPILGQITYNSIAARFARTLSTTFAAGVPLIDALKSVAGATGNVVYEEATLKIRDSVATGIP
LNGAMRVSGLYPSMLVQMAAIGEESGALDEMLAKAADFYEEAVDNLVDNLTTLMEPMIMAVLGILVGGLMIAMYLPIFQL
GQVV

Nucleotide


Download         Length: 1215 bp        

>NTDB_id=409592 GNX18_RS00645 WP_166214792.1 131480..132694(-) (pilC) [Microbulbifer sp. SH-1]
ATGGCCAAAGCCGCCGTTGCAGTTGCTTACATATACAAAGGCGTGGATGCCAAAGGGAACAAGGTTGAAGGAGAAATCAA
CTCAACCAATCCGGCATTGATCAAGGCCCAGTTGCGCCGCCAGGGAATCATCGCCAACAAAGTACAGAAAAAAGCCACCC
CGTTGTTTGGTGGCAACAAAAAAATCAAACCGGGTGATATCGCCATCTTCACTCGCCAGATGGCTACCATGATGAAGGCC
GGCGTGCCACTAGTACAGAGTTTTGAGATTGTGGCGGATGGTCTCGAAAATGCTGGCATGAAAGAGCTGATATTCAAGAT
TCGCGATGATGTTGCTTCAGGCACAGCTTTTGCTGACTCCCTGCGTAGACACTCACTGTATTTTGATGATCTATTCTGCA
ACCTGGTGGCCTCCGGCGAGCAGTCAGGTGCACTGGAAACCATGCTCGACCGCATAGCAACCTACAAGGAAAAAACCGAG
TCACTGAAGAAAAAAATCAAAAAGGCTATGACCTATCCTATAGCGGTAGTTGTTGTTGCCGTTGTGGTCACTGCAATCCT
ACTGGTAAAAGTTGTCCCCCAGTTTGCCGAAACCTTTTCCAGTTTTGGAGCAGACCTTCCCGCATTCACTCTGTTTGTTT
TGGGCATTTCCGAATGGATGCAGGCTAACTGGTTCTATGCTTTTCTGGGTGTGGTTGTCGGCATCGGCGGTACCATAGAA
GTCAAAAAGCGTAATAAAAATGTGGCCAATTTCTTTGACAAGCTGATGCTCAAGTTGCCTATTCTTGGCCAGATCACCTA
CAACTCCATTGCCGCTCGCTTTGCCCGCACACTTTCCACCACTTTCGCCGCCGGTGTCCCACTAATCGACGCACTGAAAT
CCGTTGCCGGAGCTACCGGCAATGTGGTGTACGAAGAGGCGACCCTTAAAATTCGCGATTCCGTGGCAACTGGTATACCG
TTAAACGGCGCCATGCGTGTATCTGGATTGTACCCCTCGATGCTTGTACAAATGGCTGCGATCGGCGAGGAATCCGGCGC
GCTGGATGAAATGCTGGCCAAAGCTGCCGACTTTTACGAGGAGGCTGTCGACAATTTGGTGGACAACCTGACTACACTAA
TGGAACCCATGATCATGGCCGTCTTGGGTATTCTTGTTGGTGGTTTGATGATCGCCATGTACCTCCCCATCTTCCAACTC
GGCCAGGTCGTGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(70-193)

Type II secretion system (T2SS), protein F (T2SSF)

(274-396)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6G8DA54

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

62.963

100

0.631
  pilC Acinetobacter baylyi ADP1

59.901

100

0.599
  pilC Acinetobacter baumannii D1279779

59.406

100

0.594
  pilC Legionella pneumophila strain ERS1305867

56.5

99.01

0.559
  pilG Neisseria gonorrhoeae MS11

42.172

98.02

0.413
  pilG Neisseria meningitidis 44/76-A

41.919

98.02

0.411
  pilC Vibrio cholerae strain A1552

41.414

98.02

0.406
  pilC Vibrio campbellii strain DS40M4

40.704

98.515

0.401
  pilC Thermus thermophilus HB27

35.252

100

0.364

Multiple sequence alignment