Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GPY11_RS16930 Genome accession   NZ_CP046851
Coordinates   2904076..2905770 (-) Length   564 a.a.
NCBI ID   WP_158175123.1    Uniprot ID   -
Organism   Grimontia hollisae strain F9489     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2899076..2910770
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY11_RS16900 mutT 2899668..2900072 (+) 405 WP_005502113.1 8-oxo-dGTP diphosphatase MutT -
  GPY11_RS16905 yacG 2900275..2900472 (-) 198 WP_115660105.1 DNA gyrase inhibitor YacG -
  GPY11_RS16910 zapD 2900507..2901247 (-) 741 WP_005502110.1 cell division protein ZapD -
  GPY11_RS16915 coaE 2901237..2901860 (-) 624 WP_005502108.1 dephospho-CoA kinase -
  GPY11_RS16920 pilD 2901864..2902733 (-) 870 WP_114996182.1 prepilin peptidase Machinery gene
  GPY11_RS16925 pilC 2902841..2904064 (-) 1224 WP_114996181.1 type II secretion system F family protein Machinery gene
  GPY11_RS16930 pilB 2904076..2905770 (-) 1695 WP_158175123.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GPY11_RS16935 pilA 2905794..2906231 (-) 438 WP_158175124.1 pilin Machinery gene
  GPY11_RS16940 nadC 2906459..2907364 (-) 906 WP_158161900.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GPY11_RS16945 ampD 2907556..2908098 (+) 543 WP_158175125.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GPY11_RS16950 pdhR 2908417..2909190 (+) 774 WP_005502094.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 564 a.a.        Molecular weight: 62024.56 Da        Isoelectric Point: 5.4799

>NTDB_id=408920 GPY11_RS16930 WP_158175123.1 2904076..2905770(-) (pilB) [Grimontia hollisae strain F9489]
MNYSLAVALRQAGYISPVQEQEVIANIKAEGVSAPSALLALHILSSEALANTVSELFSEPLHSIKDYPWENTSQFVNQQD
LVLSSQALPLEIKHHQLFLGLSDPTRLDIQDDFQFATGYSIMPVLLEHAQIEGAIRRLYGKNVTERVKTSGVSDSELEAL
STNSDEITDTDDLFSDDAPVSRYIQQVLLDAVRKQASDIHFEPFEQRYQIRFRLDGILQTHSTPPVGISRRLSTRLKIIA
KLNIAERRLPQDGRIKLNLSENKSIDMRVSTLPTLWGEKVVLRILDGGSVALDIDKLGYSQQQKDDYLAALDRPQGMILM
TGPTGSGKTVSLYTGLKYLNTAERNISTAEDPVEINLPGINQVNINNDIGLDFAKALRAFLRQDPDVVMVGEIRDLETAE
IGIKAAQTGHLVLSTLHTNSAAESVTRLSNMGVAPYNLAASLTLIIAQRLGRRLCQHCKVPHPPGDRHLTALTQHYPSLR
PEQVYQASPEGCDHCNNGYLGRVGIYEVMPITRELAEAIGNGASASRIETLAMGNGMLTLPLSGIEKLNEGITSLAELQR
VISL

Nucleotide


Download         Length: 1695 bp        

>NTDB_id=408920 GPY11_RS16930 WP_158175123.1 2904076..2905770(-) (pilB) [Grimontia hollisae strain F9489]
ATGAACTACAGCCTTGCTGTCGCCCTGCGCCAGGCCGGTTATATTTCCCCGGTGCAGGAGCAAGAGGTGATAGCCAACAT
CAAAGCCGAGGGTGTCAGCGCACCGTCGGCTTTGCTCGCTTTACATATTCTGAGCAGTGAAGCACTGGCAAACACGGTAT
CCGAGCTTTTCAGTGAGCCGCTGCACAGTATTAAAGATTATCCCTGGGAAAACACCAGCCAGTTTGTCAACCAACAGGAT
CTGGTGCTCTCCAGCCAGGCCCTACCGCTGGAGATTAAACACCACCAGCTGTTTCTGGGCCTCAGCGACCCCACCCGCCT
GGATATTCAGGATGATTTCCAGTTTGCCACCGGTTATTCGATTATGCCGGTGCTGCTGGAACATGCCCAAATAGAAGGAG
CAATCCGCCGCCTGTACGGCAAAAATGTCACAGAGCGGGTCAAAACCAGCGGCGTCTCGGATTCCGAACTGGAAGCGCTC
AGCACCAACAGTGATGAGATAACAGACACCGACGATCTGTTTTCCGATGATGCCCCGGTCAGCCGTTATATCCAGCAGGT
TCTTTTGGATGCGGTGCGCAAACAAGCCTCCGATATTCACTTTGAACCCTTTGAACAGCGCTACCAGATACGTTTCCGTC
TCGATGGCATTTTGCAAACCCACAGCACACCGCCGGTGGGGATCTCCCGCCGCCTTTCTACACGCCTGAAAATCATCGCC
AAGCTCAATATTGCCGAGCGCCGGCTGCCGCAGGATGGGAGAATCAAGCTCAACCTGTCGGAAAACAAATCCATCGATAT
GCGGGTCTCGACCCTGCCTACTCTGTGGGGAGAAAAAGTGGTGCTGCGAATCCTTGATGGCGGCAGTGTGGCACTGGATA
TCGACAAGCTGGGTTATAGCCAACAGCAAAAAGACGACTACCTTGCCGCCCTCGATCGCCCACAGGGGATGATCTTGATG
ACCGGGCCAACAGGCAGCGGGAAAACCGTCTCCTTGTATACCGGGCTGAAATACTTAAATACCGCTGAGCGCAATATTTC
TACCGCAGAAGACCCGGTGGAAATCAACTTGCCCGGTATCAATCAGGTCAATATCAACAACGATATCGGGCTCGACTTTG
CCAAGGCATTGCGGGCCTTCTTGCGCCAGGATCCTGATGTGGTGATGGTGGGTGAAATCCGGGATTTGGAAACCGCAGAA
ATCGGCATTAAAGCAGCGCAAACCGGCCATTTGGTGCTTTCCACCCTTCACACCAACTCTGCCGCAGAGAGTGTCACACG
TCTGTCGAACATGGGGGTTGCCCCTTACAACCTGGCTGCGTCCCTGACCCTGATTATTGCCCAGCGTCTGGGACGCCGGC
TGTGCCAGCACTGTAAAGTGCCACATCCTCCCGGCGATCGCCACCTGACAGCGCTCACCCAGCATTATCCTTCGCTGAGG
CCGGAACAGGTTTACCAGGCATCGCCTGAAGGCTGTGACCATTGCAACAACGGCTATCTGGGACGGGTGGGAATTTATGA
GGTGATGCCCATCACCCGTGAGCTTGCCGAAGCCATTGGCAATGGCGCATCGGCATCACGAATTGAAACCCTCGCCATGG
GCAATGGCATGTTAACACTGCCACTTTCCGGCATTGAGAAACTAAATGAAGGCATCACCAGCCTTGCTGAACTGCAACGT
GTGATCAGCCTGTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-141)

Type II/IV secretion system protein (T2SSE)

(186-453)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

58.407

100

0.585
  pilB Vibrio campbellii strain DS40M4

57.092

100

0.571
  pilB Vibrio parahaemolyticus RIMD 2210633

56.56

100

0.566
  pilB Acinetobacter baylyi ADP1

46.986

100

0.47
  pilB Legionella pneumophila strain ERS1305867

48.237

95.567

0.461
  pilB Acinetobacter baumannii D1279779

48.771

93.794

0.457
  pilF Neisseria gonorrhoeae MS11

43.303

96.631

0.418
  pilF Thermus thermophilus HB27

37.702

98.759

0.372

Multiple sequence alignment