Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   DELCS14_RS27985 Genome accession   NC_015563
Coordinates   6366087..6366611 (+) Length   174 a.a.
NCBI ID   WP_013804585.1    Uniprot ID   -
Organism   Delftia sp. Cs1-4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6361087..6371611
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DELCS14_RS27965 (DelCs14_5658) sucD 6362080..6362973 (+) 894 WP_012202800.1 succinate--CoA ligase subunit alpha -
  DELCS14_RS27970 (DelCs14_5659) - 6363140..6363832 (+) 693 WP_013804582.1 TerC family protein -
  DELCS14_RS27975 (DelCs14_5660) - 6364125..6364907 (+) 783 WP_013804583.1 protein phosphatase 2C domain-containing protein -
  DELCS14_RS27980 (DelCs14_5661) - 6365152..6365862 (+) 711 WP_013804584.1 FHA domain-containing protein -
  DELCS14_RS27985 (DelCs14_5662) pilE 6366087..6366611 (+) 525 WP_013804585.1 pilin Machinery gene
  DELCS14_RS30930 (DelCs14_5663) - 6366961..6368508 (+) 1548 WP_157158323.1 hypothetical protein -
  DELCS14_RS30275 (DelCs14_5664) - 6368731..6369483 (-) 753 WP_013804587.1 SDR family NAD(P)-dependent oxidoreductase -
  DELCS14_RS30280 (DelCs14_5665) - 6369485..6370792 (-) 1308 WP_013804588.1 FAD-binding oxidoreductase -
  DELCS14_RS28005 (DelCs14_5666) - 6370789..6371202 (-) 414 WP_013804589.1 GtrA family protein -

Sequence

Protein


Download         Length: 174 a.a.        Molecular weight: 17913.59 Da        Isoelectric Point: 9.0311

>NTDB_id=40837 DELCS14_RS27985 WP_013804585.1 6366087..6366611(+) (pilE) [Delftia sp. Cs1-4]
MKRSIQQGFTLIELMIVVAIIGILAAVALPAYQDYTVRAKVSEAVIQATSPKSLISEAFQTDGLSGMVAAAATFNSKPSN
EKATKYVADMTIDSANGQITVTLQSGTSAGFPSDVRGKTLVFTPNVQKVALADGKQGAIDWACGSTTAATAGTRLLGAAA
LGTLPAKYAPAECR

Nucleotide


Download         Length: 525 bp        

>NTDB_id=40837 DELCS14_RS27985 WP_013804585.1 6366087..6366611(+) (pilE) [Delftia sp. Cs1-4]
ATGAAGCGTTCCATCCAGCAAGGTTTTACCTTGATCGAATTGATGATCGTTGTGGCGATCATCGGCATCTTGGCAGCAGT
TGCGCTGCCGGCTTATCAGGATTACACGGTGCGAGCCAAAGTCAGCGAAGCCGTAATTCAAGCCACATCTCCAAAGTCTC
TGATCAGCGAAGCTTTCCAGACTGACGGCCTTTCGGGCATGGTGGCAGCGGCCGCAACATTCAACAGCAAACCCAGCAAC
GAGAAGGCGACCAAGTATGTTGCAGATATGACCATCGATTCTGCAAATGGTCAAATCACTGTTACATTGCAAAGTGGTAC
TAGCGCCGGCTTCCCCTCCGATGTGCGTGGAAAGACCCTGGTCTTTACGCCCAATGTGCAAAAGGTTGCGCTGGCCGATG
GCAAGCAAGGTGCCATCGACTGGGCTTGTGGCTCGACCACTGCAGCTACTGCAGGCACCCGCCTGCTGGGTGCTGCAGCA
CTCGGTACTTTGCCAGCGAAGTACGCACCTGCTGAGTGCCGTTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(37-174)

Prokaryotic N-terminal methylation motif (N_methyl)

(4-29)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

50.802

100

0.546
  pilA Ralstonia pseudosolanacearum GMI1000

42.458

100

0.437
  pilA2 Legionella pneumophila strain ERS1305867

41.477

100

0.42
  pilA2 Legionella pneumophila str. Paris

40.909

100

0.414
  pilE Neisseria gonorrhoeae strain FA1090

37.079

100

0.379
  comP Acinetobacter baylyi ADP1

37.714

100

0.379
  pilE Neisseria gonorrhoeae MS11

37.209

98.851

0.368
  pilA/pilAI Pseudomonas stutzeri DSM 10701

36.207

100

0.362

Multiple sequence alignment