Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   GPY28_RS23495 Genome accession   NZ_CP046814
Coordinates   3061240..3062463 (+) Length   407 a.a.
NCBI ID   WP_017635918.1    Uniprot ID   A0AAW9BMM9
Organism   Vibrio alginolyticus strain 2010V-1102     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3056240..3067463
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY28_RS23475 ampD 3057306..3057857 (-) 552 WP_042521337.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GPY28_RS23480 nadC 3057950..3058837 (+) 888 WP_158159766.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GPY28_RS23485 pilA 3059103..3059531 (+) 429 WP_050912911.1 pilin Machinery gene
  GPY28_RS23490 pilB 3059531..3061216 (+) 1686 WP_158159767.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GPY28_RS23495 pilC 3061240..3062463 (+) 1224 WP_017635918.1 type II secretion system F family protein Machinery gene
  GPY28_RS23500 pilD 3062528..3063397 (+) 870 WP_158159768.1 A24 family peptidase Machinery gene
  GPY28_RS23505 coaE 3063398..3064012 (+) 615 WP_158159769.1 dephospho-CoA kinase -
  GPY28_RS23510 zapD 3064040..3064780 (+) 741 WP_005373086.1 cell division protein ZapD -
  GPY28_RS23515 yacG 3064939..3065133 (+) 195 WP_017635922.1 DNA gyrase inhibitor YacG -
  GPY28_RS23520 rplS 3065788..3066141 (-) 354 WP_005379971.1 50S ribosomal protein L19 -
  GPY28_RS23525 trmD 3066183..3066944 (-) 762 WP_005385420.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -

Sequence

Protein


Download         Length: 407 a.a.        Molecular weight: 45313.50 Da        Isoelectric Point: 10.3992

>NTDB_id=408237 GPY28_RS23495 WP_017635918.1 3061240..3062463(+) (pilC) [Vibrio alginolyticus strain 2010V-1102]
MKSTTPQLKNFRWKGINSSGKKTSGQTLAMTEIEVRERLDAQHIKIKKLKKSSISFLTKLSHRVKGRDITVFTRQISTML
ITGVPLVQALKLVSENHKKAEMKSILMSVTRAVEAGTPMSKAMRTASEHFDPLYTDLIATGEQSGNLAEVFERLATYREK
NEQLRAKVIKALIYPAMVVLVALGVSFIMLTKVIPEFEKMFVGFGADLPWFTRQVLDLSAWTQNWSPFIALGSISLFISA
RILSKRSDSFRLMLNRSVLKFPVLGPVLSKAAIAKFSRTLATSFTAGIPILTSLKTTSKTSGNLHYQLAIEEVYRDTAAG
MPMYVAMRNCNVFPELVLQMVMIGEESGRLDDMLNKVATIYEFEVDNTVDNLSKILEPLIIVFLGIVVGGLVTAMYLPIF
NLMSVLG

Nucleotide


Download         Length: 1224 bp        

>NTDB_id=408237 GPY28_RS23495 WP_017635918.1 3061240..3062463(+) (pilC) [Vibrio alginolyticus strain 2010V-1102]
ATGAAAAGCACTACACCACAACTTAAAAACTTCCGCTGGAAAGGCATCAACAGTTCTGGCAAAAAGACGTCTGGACAAAC
CCTCGCGATGACAGAAATTGAAGTACGCGAGCGTCTAGACGCACAGCACATCAAGATCAAGAAGTTAAAGAAAAGCAGTA
TCTCGTTTCTCACTAAACTCAGCCATCGCGTGAAAGGCAGAGACATCACGGTGTTTACCCGTCAGATTTCGACGATGTTG
ATAACCGGTGTACCCTTAGTTCAGGCTTTAAAATTGGTCTCAGAAAATCATAAAAAAGCAGAGATGAAATCCATTTTGAT
GAGCGTGACACGCGCCGTTGAAGCGGGGACGCCCATGTCAAAAGCCATGCGCACTGCAAGTGAACACTTTGACCCACTCT
ATACCGATCTTATCGCCACAGGTGAGCAATCCGGTAACTTAGCAGAAGTGTTCGAGCGCTTAGCCACCTACCGAGAAAAA
AATGAACAACTCCGTGCGAAAGTGATCAAAGCACTGATCTACCCAGCCATGGTAGTTCTAGTAGCGTTAGGCGTGTCGTT
TATCATGCTCACCAAAGTCATTCCCGAGTTTGAAAAAATGTTTGTTGGTTTTGGTGCTGACTTACCGTGGTTTACCAGGC
AAGTATTAGATCTTTCCGCCTGGACACAAAACTGGAGTCCGTTTATCGCACTAGGCTCCATTAGTTTATTCATTTCGGCG
AGAATCCTCTCTAAGCGTTCAGATTCTTTTCGCTTAATGCTCAACCGCTCTGTGCTTAAATTTCCGGTCCTTGGGCCTGT
ATTATCAAAAGCCGCTATCGCCAAATTTAGTCGAACACTCGCCACAAGCTTTACAGCGGGTATTCCAATTCTAACCAGTT
TAAAAACCACATCAAAAACCTCAGGAAACTTACACTATCAACTCGCCATAGAAGAAGTTTACCGTGATACCGCCGCAGGT
ATGCCAATGTATGTTGCCATGCGTAACTGTAATGTGTTCCCTGAATTAGTGTTACAAATGGTCATGATCGGTGAAGAGTC
TGGTCGACTTGACGATATGCTCAATAAAGTTGCAACGATCTACGAGTTTGAAGTCGACAACACCGTCGACAACCTTAGTA
AAATTCTAGAGCCACTAATTATCGTATTTTTAGGTATCGTTGTTGGCGGATTAGTGACAGCAATGTACTTGCCAATCTTT
AACTTAATGAGTGTATTGGGCTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(72-195)

Type II secretion system (T2SS), protein F (T2SSF)

(276-398)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio campbellii strain DS40M4

87.224

100

0.872
  pilC Vibrio cholerae strain A1552

73.775

100

0.74
  pilC Acinetobacter baylyi ADP1

43

98.28

0.423
  pilC Acinetobacter baumannii D1279779

41.439

99.017

0.41
  pilG Neisseria gonorrhoeae MS11

40.494

99.509

0.403
  pilC Legionella pneumophila strain ERS1305867

40.295

100

0.403
  pilG Neisseria meningitidis 44/76-A

40.247

99.509

0.4
  pilC Pseudomonas stutzeri DSM 10701

40.909

97.297

0.398

Multiple sequence alignment