Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GPY47_RS15630 Genome accession   NZ_CP046806
Coordinates   1622312..1623997 (+) Length   561 a.a.
NCBI ID   WP_054576330.1    Uniprot ID   -
Organism   Vibrio alginolyticus strain 2439-01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1617312..1628997
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY47_RS15610 pdhR 1618882..1619649 (-) 768 WP_005379989.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  GPY47_RS15615 ampD 1620055..1620606 (-) 552 WP_042521337.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GPY47_RS15620 nadC 1620699..1621586 (+) 888 WP_054576329.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GPY47_RS15625 pilA 1621848..1622306 (+) 459 WP_047008977.1 pilin Machinery gene
  GPY47_RS15630 pilB 1622312..1623997 (+) 1686 WP_054576330.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GPY47_RS15635 pilC 1624021..1625244 (+) 1224 WP_158156851.1 type II secretion system F family protein Machinery gene
  GPY47_RS15640 pilD 1625309..1626178 (+) 870 WP_158156853.1 A24 family peptidase Machinery gene
  GPY47_RS15645 coaE 1626179..1626793 (+) 615 WP_064354292.1 dephospho-CoA kinase -
  GPY47_RS15650 zapD 1626821..1627561 (+) 741 WP_158156855.1 cell division protein ZapD -
  GPY47_RS15655 yacG 1627720..1627914 (+) 195 WP_017635922.1 DNA gyrase inhibitor YacG -
  GPY47_RS15660 rplS 1628398..1628751 (-) 354 WP_005379971.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62118.92 Da        Isoelectric Point: 5.4031

>NTDB_id=408092 GPY47_RS15630 WP_054576330.1 1622312..1623997(+) (pilB) [Vibrio alginolyticus strain 2439-01]
MLTNLSTILRQKGLLTFSQEESLIEQVKASGISMPEALLSSGLFTSSELAEHLSSLFGLNQPELSQYEYASLCQQLGLRE
LITRHNALPLQRTSSTLLLAVADPTNQQAEDDFRFATGLQVELVLGEFSQLSAAIRRLYGRSLSHEKSGLKEINQEELAN
LVDVGADEIDNIEDLSQDESPVSRYINQILLDAVRKGASDIHFEPYEKMYRVRLRCDGILIETQQPPSHLSRRLSARIKI
LSKLDIAERRLPQDGRIKLKLNQDTAIDMRVSTLPTLFGEKIVLRLLDSSSASLDIDKLGYSEQQKQLYLDALRRPQGMI
LMTGPTGSGKTVSLYTGLSILNKPEINISTAEDPVEINLSGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLDT
AEIAIKASQTGHLVLSTLHTNSAAETVIRLSNMGVESFNLASSLSLIIAQRLARKLCSHCKQSQELTVQLQHLGIQASDN
IFKANPDGCNECTHGYSGRTGIYEVMRFDEFLSEALIKGASVHELEKLAIANGMSTLQMSGIEKLKQGITSFSELQRVLY
F

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=408092 GPY47_RS15630 WP_054576330.1 1622312..1623997(+) (pilB) [Vibrio alginolyticus strain 2439-01]
ATGCTAACCAACCTCTCAACCATTCTTCGCCAAAAAGGGTTACTGACGTTTTCCCAAGAAGAATCGTTAATAGAGCAAGT
CAAAGCTTCTGGCATTTCGATGCCAGAGGCTTTACTCAGTTCTGGATTATTTACATCAAGCGAACTCGCTGAGCACTTAA
GTTCTCTCTTTGGTTTAAATCAGCCCGAGTTATCTCAGTACGAGTATGCTTCGCTTTGTCAGCAACTTGGCCTTAGGGAA
TTAATCACGCGGCATAACGCACTCCCACTCCAGCGTACGTCTTCAACGTTATTACTCGCCGTTGCCGACCCCACCAACCA
ACAAGCAGAAGATGATTTTCGCTTTGCCACTGGTTTACAGGTTGAATTGGTCTTGGGGGAATTTAGTCAACTCAGCGCGG
CAATTCGACGCTTGTATGGTCGTTCATTGAGTCATGAGAAGTCCGGGTTAAAAGAGATCAACCAAGAAGAGTTAGCCAAT
CTGGTTGACGTGGGCGCAGATGAAATCGACAACATTGAAGACTTGAGCCAAGATGAGTCTCCTGTTAGCCGTTACATCAA
CCAAATTCTATTGGACGCTGTGCGCAAAGGCGCATCCGATATCCACTTTGAGCCTTATGAAAAGATGTACCGCGTGCGCC
TGCGCTGTGATGGCATCTTAATTGAAACACAGCAGCCACCAAGCCACTTGAGTCGCCGCTTATCCGCTCGTATCAAAATC
CTCTCTAAGCTTGATATTGCAGAGCGACGTTTACCACAAGATGGGCGTATTAAGCTCAAGTTGAATCAAGACACCGCAAT
TGATATGCGTGTATCCACGCTGCCTACTCTATTTGGAGAGAAAATTGTACTACGACTGCTCGACAGCAGCTCTGCGTCAT
TGGATATCGACAAGCTTGGTTATAGCGAACAACAAAAACAATTGTACTTGGATGCGCTGCGTCGCCCACAAGGCATGATT
CTGATGACAGGTCCAACCGGGAGCGGAAAAACCGTTTCTTTGTACACCGGGCTGAGTATCCTCAATAAACCAGAAATCAA
CATTTCTACGGCAGAAGACCCAGTAGAAATTAACTTATCGGGCATTAACCAAGTTCAAGTCCAGCCTAAGATTGGTTTTG
GTTTTGCCGAAGCACTGCGCTCTTTCTTACGTCAAGACCCCGATGTGGTAATGGTCGGTGAGATTCGTGACTTAGACACT
GCAGAAATCGCGATCAAAGCCTCGCAAACTGGTCACTTAGTACTTTCAACCTTACATACCAATTCTGCGGCTGAAACGGT
TATTCGTTTATCAAACATGGGCGTAGAAAGCTTTAACTTGGCCTCATCTCTTAGTTTGATTATTGCCCAGCGCTTAGCCC
GTAAACTGTGCTCGCACTGTAAACAATCACAAGAGCTTACCGTTCAGCTACAACATCTTGGAATTCAAGCAAGCGACAAC
ATTTTTAAAGCCAATCCCGATGGATGCAATGAGTGTACCCATGGTTATTCCGGCCGAACGGGGATTTATGAAGTGATGCG
CTTTGATGAATTTCTTTCCGAAGCGCTTATTAAAGGTGCCTCGGTACATGAACTAGAAAAACTCGCCATCGCAAATGGTA
TGAGCACGTTACAAATGTCGGGCATAGAAAAACTCAAACAAGGGATCACAAGTTTTAGTGAACTACAGCGCGTACTTTAT
TTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio parahaemolyticus RIMD 2210633

95.544

100

0.955
  pilB Vibrio campbellii strain DS40M4

89.84

100

0.898
  pilB Vibrio cholerae strain A1552

74.199

100

0.743
  pilB Acinetobacter baumannii D1279779

51.81

93.583

0.485
  pilB Legionella pneumophila strain ERS1305867

49.538

96.435

0.478
  pilB Acinetobacter baylyi ADP1

50.484

92.157

0.465
  pilF Neisseria gonorrhoeae MS11

45.213

100

0.455
  pilB/pilB1 Synechocystis sp. PCC 6803

37.225

100

0.392
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.508

100

0.369
  pilF Thermus thermophilus HB27

36.121

100

0.362

Multiple sequence alignment