Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   GPY59_RS23485 Genome accession   NZ_CP046772
Coordinates   3134142..3135887 (-) Length   581 a.a.
NCBI ID   WP_199266743.1    Uniprot ID   -
Organism   Vibrio alginolyticus strain 2014V-1011     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3129142..3140887
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY59_RS23465 - 3129830..3130669 (-) 840 WP_005381301.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  GPY59_RS23470 - 3130739..3132250 (-) 1512 WP_158174190.1 AAA family ATPase -
  GPY59_RS23475 aroB 3132303..3133403 (-) 1101 WP_005381318.1 3-dehydroquinate synthase -
  GPY59_RS23480 aroK 3133436..3133954 (-) 519 WP_005381319.1 shikimate kinase AroK -
  GPY59_RS23485 pilQ 3134142..3135887 (-) 1746 WP_199266743.1 type IV pilus secretin PilQ family protein Machinery gene
  GPY59_RS23490 pilP 3135913..3136428 (-) 516 WP_115386334.1 pilus assembly protein PilP Machinery gene
  GPY59_RS23495 pilO 3136418..3137011 (-) 594 WP_158174192.1 type 4a pilus biogenesis protein PilO Machinery gene
  GPY59_RS23500 pilN 3137004..3137579 (-) 576 WP_005381323.1 PilN domain-containing protein Machinery gene
  GPY59_RS23505 pilM 3137563..3138579 (-) 1017 WP_005381324.1 type IV pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 581 a.a.        Molecular weight: 63741.79 Da        Isoelectric Point: 4.4809

>NTDB_id=407483 GPY59_RS23485 WP_199266743.1 3134142..3135887(-) (pilQ) [Vibrio alginolyticus strain 2014V-1011]
MKMRQGLTRTPRILTGLFSLWLFVFLAPLAAAEEVSSNALKSIDFRTNKDKDAVIVVELASPAAIVDIKRVQEGLSIDLL
NTSVKDEQLYLLDVKDFSTVVESVEVFRYTSTTRLVAHINEEYMHDYRLTGRFLEIKVAKIKPNEKAPDKSILEREGKLI
SINFQDIPVRNVLQLIADYNQFNLVVSDSVEGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPTDELDLREKQQ
LEKQKLTEEMGELSSEIIKVNFAKASDIAEMINGEGNISMLSERGSMTIDERTNSLLIRELPENIEVIREIIESLDIPVK
QVQIEARIVTINEGNMDELGIRWGFTSINGSNTVGGSIENNLATIGLYNGEGGDDGGDGDGVGIDDFLNVNLAATSPNAS
SIAFQVAKLGSDTLLDLELSALQQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATTVTFKKAVLSLKVTPQI
TPDNRLVLDLSVTQDRPGQVVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSITNSVDKVPLLGDLPLLGALFRR
SYENVGKSELLIFVTPKVVIQ

Nucleotide


Download         Length: 1746 bp        

>NTDB_id=407483 GPY59_RS23485 WP_199266743.1 3134142..3135887(-) (pilQ) [Vibrio alginolyticus strain 2014V-1011]
ATGAAAATGAGACAAGGATTAACACGTACACCAAGGATACTTACTGGGCTCTTCTCCTTATGGTTGTTTGTATTTTTAGC
CCCTTTAGCGGCAGCTGAAGAAGTTTCGTCCAATGCCCTAAAAAGTATCGACTTTCGAACTAATAAAGATAAAGACGCCG
TGATTGTCGTCGAATTGGCGTCACCGGCCGCGATTGTAGATATCAAGCGTGTACAGGAAGGGTTAAGTATCGATTTGCTT
AACACTTCAGTAAAAGACGAGCAATTGTATCTCCTAGACGTGAAAGACTTTTCGACTGTGGTTGAGAGCGTAGAAGTGTT
TCGCTATACCTCAACTACGCGCTTGGTTGCGCACATCAATGAAGAATACATGCATGATTACCGTTTAACGGGGCGTTTTT
TGGAAATCAAAGTAGCGAAGATCAAACCCAATGAGAAAGCCCCAGATAAAAGCATTTTAGAAAGAGAAGGTAAGCTTATT
TCGATTAACTTCCAAGATATTCCTGTGCGAAACGTCTTGCAGCTTATTGCTGATTACAACCAATTCAATTTGGTTGTCTC
CGACTCTGTAGAAGGCAACCTCACGCTTCGTCTTGATGGTGTCCCTTGGCAGCAAGTGCTGGATATTATTTTGCAAGTTA
AGGGGTTAGATAAGCGTGTTGATGGCAACGTGATTTTAGTTGCTCCGACTGATGAGTTGGACTTGCGTGAGAAGCAGCAG
TTAGAAAAACAAAAACTGACGGAAGAAATGGGAGAGCTTTCATCTGAAATCATCAAAGTTAATTTTGCTAAAGCATCAGA
CATTGCGGAGATGATCAATGGTGAAGGAAATATCAGCATGCTGTCAGAGCGCGGCAGTATGACCATTGATGAGCGAACTA
ACTCGTTATTGATTAGAGAGTTACCTGAAAACATCGAGGTGATTCGAGAAATTATTGAGTCTTTGGATATTCCAGTTAAA
CAGGTTCAAATTGAGGCGCGTATTGTGACCATCAATGAAGGTAACATGGACGAGTTAGGAATTCGTTGGGGTTTTACGTC
TATTAATGGCAGTAATACAGTGGGCGGTTCTATTGAGAACAACCTCGCCACGATCGGTCTCTATAATGGAGAAGGGGGTG
ATGACGGTGGTGACGGTGATGGAGTAGGGATCGATGACTTCCTAAATGTAAACTTAGCTGCAACTAGCCCGAATGCGAGC
AGCATTGCTTTTCAGGTCGCAAAGTTAGGTTCAGACACCTTGTTGGATCTTGAGCTTTCTGCTCTGCAACAAGAGTCTAA
AGCTGAGATTATCTCGAGCCCACGTTTGATCACTACCAATAAGAAGCCTGCCTATATTGAACAAGGTACAGAGATTCCTT
ATCTTGAGTCATCATCAAGTGGTGCAACGACGGTGACGTTCAAAAAGGCGGTATTAAGTTTGAAAGTGACCCCGCAAATC
ACACCGGATAACCGCTTAGTGTTGGATTTAAGTGTCACGCAAGATAGACCTGGACAAGTCGTGAAGACAGGCACTGGTGA
AGCGGTTGCGATTGATACTCAACGTATCGGGACGCAAGTGTTAGTGAATAACGGTGAAACGGTCGTTCTTGGTGGTATTT
TTCAGCACAGCATTACTAACTCGGTGGACAAAGTACCTTTGTTGGGCGACCTGCCGCTATTAGGTGCTCTTTTTCGTCGA
AGTTATGAGAATGTTGGCAAAAGTGAACTGTTAATTTTCGTCACGCCTAAGGTGGTGATTCAGTAG

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(256-321)

Bacterial type II and III secretion system protein (Secretin)

(421-579)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

92.599

100

0.926
  pilQ Vibrio cholerae strain A1552

74.914

100

0.75
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

74.914

100

0.75
  pilQ Pseudomonas aeruginosa PAK

39.415

100

0.394
  pilQ Legionella pneumophila strain ERS1305867

40.608

96.213

0.391
  pilQ Legionella pneumophila strain Lp02

40.608

96.213

0.391
  comQ Acinetobacter baylyi ADP1

37.414

99.828

0.373
  pilQ Acinetobacter baumannii strain A118

36.074

100

0.37
  pilQ Acinetobacter baumannii D1279779

36.074

100

0.37

Multiple sequence alignment