Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   GPY59_RS23495 Genome accession   NZ_CP046772
Coordinates   3136418..3137011 (-) Length   197 a.a.
NCBI ID   WP_158174192.1    Uniprot ID   -
Organism   Vibrio alginolyticus strain 2014V-1011     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3131418..3142011
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY59_RS23475 aroB 3132303..3133403 (-) 1101 WP_005381318.1 3-dehydroquinate synthase -
  GPY59_RS23480 aroK 3133436..3133954 (-) 519 WP_005381319.1 shikimate kinase AroK -
  GPY59_RS23485 pilQ 3134142..3135887 (-) 1746 WP_199266743.1 type IV pilus secretin PilQ family protein Machinery gene
  GPY59_RS23490 pilP 3135913..3136428 (-) 516 WP_115386334.1 pilus assembly protein PilP Machinery gene
  GPY59_RS23495 pilO 3136418..3137011 (-) 594 WP_158174192.1 type 4a pilus biogenesis protein PilO Machinery gene
  GPY59_RS23500 pilN 3137004..3137579 (-) 576 WP_005381323.1 PilN domain-containing protein Machinery gene
  GPY59_RS23505 pilM 3137563..3138579 (-) 1017 WP_005381324.1 type IV pilus assembly protein PilM Machinery gene
  GPY59_RS23510 - 3138724..3141285 (+) 2562 WP_065274916.1 PBP1A family penicillin-binding protein -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 23027.54 Da        Isoelectric Point: 4.9271

>NTDB_id=407485 GPY59_RS23495 WP_158174192.1 3136418..3137011(-) (pilO) [Vibrio alginolyticus strain 2014V-1011]
MVDWQDLEIDEMAEWPLLPQIVVIALLIMAIQGLGYWFYLKPKEEQLNYLIQQEQTLKVALKIKANKVAALPQLQSQLDE
LTTRYELLLQQLPAQKELATMLASVNELGIDNKLTFTRIDWGEKQSEHFLYRLPLNIELTGDFHDIGHFSQAIATLPRII
TFKDVTWQRVSQESETLHFRVRANTYQFKPEETKNDK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=407485 GPY59_RS23495 WP_158174192.1 3136418..3137011(-) (pilO) [Vibrio alginolyticus strain 2014V-1011]
GTGGTTGATTGGCAGGATCTCGAAATAGATGAAATGGCCGAGTGGCCATTGTTGCCCCAGATTGTAGTAATCGCGCTTCT
GATCATGGCGATTCAAGGCTTAGGTTATTGGTTTTACCTTAAACCGAAAGAAGAACAGTTGAATTACTTGATCCAGCAGG
AGCAGACACTCAAGGTGGCATTGAAGATCAAAGCGAACAAAGTGGCAGCGTTGCCTCAGCTTCAATCTCAATTGGATGAA
CTTACCACTCGTTACGAGTTATTACTGCAACAACTGCCTGCGCAAAAAGAGCTGGCTACAATGCTCGCTTCAGTGAATGA
GCTTGGCATCGACAATAAGCTGACTTTCACCCGAATCGACTGGGGCGAAAAACAATCAGAGCACTTCCTTTATCGTCTTC
CTTTAAACATAGAGTTAACGGGCGACTTTCATGACATCGGCCATTTTTCTCAGGCAATAGCGACGCTACCTCGAATCATC
ACGTTTAAAGATGTGACTTGGCAACGTGTCAGCCAAGAGAGCGAAACCCTGCACTTTCGGGTGCGCGCTAACACCTATCA
GTTTAAGCCGGAGGAGACGAAAAATGACAAGTAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(50-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

87.895

96.447

0.848
  pilO Vibrio cholerae strain A1552

70.681

96.954

0.685

Multiple sequence alignment