Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   GPY59_RS22105 Genome accession   NZ_CP046772
Coordinates   2863873..2865096 (+) Length   407 a.a.
NCBI ID   WP_017820393.1    Uniprot ID   -
Organism   Vibrio alginolyticus strain 2014V-1011     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2858873..2870096
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY59_RS22085 ampD 2859921..2860472 (-) 552 WP_042521337.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GPY59_RS22090 nadC 2860565..2861452 (+) 888 WP_064354288.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GPY59_RS22095 - 2861714..2862151 (+) 438 WP_137282354.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  GPY59_RS22100 pilB 2862164..2863849 (+) 1686 WP_158174152.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GPY59_RS22105 pilC 2863873..2865096 (+) 1224 WP_017820393.1 type II secretion system F family protein Machinery gene
  GPY59_RS22110 pilD 2865161..2866030 (+) 870 WP_158174153.1 A24 family peptidase Machinery gene
  GPY59_RS22115 coaE 2866031..2866645 (+) 615 WP_158174154.1 dephospho-CoA kinase -
  GPY59_RS22120 zapD 2866673..2867413 (+) 741 WP_005373086.1 cell division protein ZapD -
  GPY59_RS22125 yacG 2867572..2867766 (+) 195 WP_017635922.1 DNA gyrase inhibitor YacG -
  GPY59_RS22130 rplS 2868709..2869062 (-) 354 WP_005379971.1 50S ribosomal protein L19 -
  GPY59_RS22135 trmD 2869104..2869865 (-) 762 WP_017635923.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -

Sequence

Protein


Download         Length: 407 a.a.        Molecular weight: 45310.50 Da        Isoelectric Point: 10.3992

>NTDB_id=407472 GPY59_RS22105 WP_017820393.1 2863873..2865096(+) (pilC) [Vibrio alginolyticus strain 2014V-1011]
MKSTTPQLKNFRWKGINSSGKKTSGQTLAMTEIEVRERLDAQHIKIKKLKKSSISFLTKLNHRVKGRDITVFTRQISTML
ITGVPLVQALKLVSENHKKAEMKSILMSVTRAVEAGAPMSKAMRTASEHFDPLYTDLIATGEQSGNLAEVFERLATYREK
NEQLRAKVIKALIYPAMVVLVALGVSFIMLTKVIPEFEKMFVGFGADLPWFTRQVLDLSAWTQNWSPFIALGSISLFISA
RILSKRSDSFRLMLNRSVLKFPVLGPVLSKAAIAKFSRTLATSFTAGIPILTSLKTTSKTSGNLHYQLAIEEVYRDTAAG
MPMYVAMRNCNVFPELVLQMVMIGEESGRLDDMLNKVATIYEFEVDNTVDNLSKILEPLIIVFLGIVVGGLVTAMYLPIF
NLMSVLG

Nucleotide


Download         Length: 1224 bp        

>NTDB_id=407472 GPY59_RS22105 WP_017820393.1 2863873..2865096(+) (pilC) [Vibrio alginolyticus strain 2014V-1011]
ATGAAAAGCACTACACCACAACTTAAAAACTTCCGCTGGAAAGGCATCAACAGTTCAGGCAAAAAGACATCTGGGCAAAC
CCTCGCGATGACAGAAATTGAAGTACGCGAGCGTCTAGACGCACAGCACATCAAGATCAAGAAGTTAAAGAAAAGCAGTA
TCTCGTTTCTCACTAAACTCAACCATCGCGTGAAAGGCAGAGACATCACGGTGTTTACCCGTCAGATTTCGACGATGTTG
ATAACCGGTGTACCCTTAGTTCAGGCTTTAAAATTAGTCTCAGAAAATCATAAAAAAGCAGAGATGAAATCCATTTTGAT
GAGCGTGACACGCGCCGTTGAAGCGGGGGCGCCCATGTCAAAAGCCATGCGCACTGCAAGTGAACACTTTGACCCACTCT
ATACTGATCTTATCGCCACAGGTGAGCAATCCGGTAACTTAGCGGAAGTGTTCGAGCGCTTAGCCACCTACCGAGAAAAA
AATGAACAACTCCGTGCGAAAGTGATCAAAGCACTGATTTACCCAGCGATGGTAGTGCTAGTAGCGTTAGGCGTGTCGTT
TATCATGCTCACCAAAGTCATTCCCGAGTTCGAAAAAATGTTTGTTGGTTTTGGTGCTGACTTACCTTGGTTTACCAGGC
AAGTGTTAGATCTTTCCGCCTGGACACAAAACTGGAGTCCGTTTATCGCACTAGGCTCCATTAGTTTATTCATTTCGGCG
AGAATCCTCTCTAAGCGTTCAGATTCTTTTCGCTTAATGCTCAACCGCTCTGTGCTTAAATTTCCGGTCCTTGGGCCTGT
ATTATCAAAAGCCGCTATCGCCAAATTTAGTCGAACACTCGCCACAAGCTTTACAGCGGGTATTCCAATTCTAACCAGTT
TAAAAACCACATCAAAAACCTCAGGAAACTTACACTATCAACTCGCCATAGAAGAAGTTTACCGTGATACCGCCGCAGGT
ATGCCAATGTATGTTGCCATGCGTAACTGTAATGTGTTCCCTGAATTAGTGTTACAAATGGTCATGATCGGTGAAGAGTC
TGGTCGACTTGACGATATGCTCAATAAAGTTGCAACGATCTACGAGTTTGAAGTCGACAACACCGTCGACAACCTTAGTA
AAATTCTAGAGCCACTAATTATCGTATTTTTAGGTATCGTTGTTGGCGGATTAGTGACAGCAATGTACTTGCCAATCTTT
AACTTAATGAGTGTATTGGGCTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(72-195)

Type II secretion system (T2SS), protein F (T2SSF)

(276-398)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio campbellii strain DS40M4

86.732

100

0.867
  pilC Vibrio cholerae strain A1552

73.529

100

0.737
  pilC Acinetobacter baylyi ADP1

43

98.28

0.423
  pilC Acinetobacter baumannii D1279779

41.439

99.017

0.41
  pilC Legionella pneumophila strain ERS1305867

40.541

100

0.405
  pilG Neisseria gonorrhoeae MS11

40.494

99.509

0.403
  pilG Neisseria meningitidis 44/76-A

40.247

99.509

0.4
  pilC Pseudomonas stutzeri DSM 10701

40.909

97.297

0.398

Multiple sequence alignment