Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GPY59_RS22100 Genome accession   NZ_CP046772
Coordinates   2862164..2863849 (+) Length   561 a.a.
NCBI ID   WP_158174152.1    Uniprot ID   -
Organism   Vibrio alginolyticus strain 2014V-1011     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2857164..2868849
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY59_RS22080 pdhR 2858748..2859515 (-) 768 WP_005379989.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  GPY59_RS22085 ampD 2859921..2860472 (-) 552 WP_042521337.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GPY59_RS22090 nadC 2860565..2861452 (+) 888 WP_064354288.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GPY59_RS22095 - 2861714..2862151 (+) 438 WP_137282354.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  GPY59_RS22100 pilB 2862164..2863849 (+) 1686 WP_158174152.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GPY59_RS22105 pilC 2863873..2865096 (+) 1224 WP_017820393.1 type II secretion system F family protein Machinery gene
  GPY59_RS22110 pilD 2865161..2866030 (+) 870 WP_158174153.1 A24 family peptidase Machinery gene
  GPY59_RS22115 coaE 2866031..2866645 (+) 615 WP_158174154.1 dephospho-CoA kinase -
  GPY59_RS22120 zapD 2866673..2867413 (+) 741 WP_005373086.1 cell division protein ZapD -
  GPY59_RS22125 yacG 2867572..2867766 (+) 195 WP_017635922.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62299.22 Da        Isoelectric Point: 5.6896

>NTDB_id=407471 GPY59_RS22100 WP_158174152.1 2862164..2863849(+) (pilB) [Vibrio alginolyticus strain 2014V-1011]
MLTKLSTVLRQKGLLTFSQEESLIEQVKASGISMPEALLSSGFFTSSELADHLSSLFGLNQSELSQYEYASLCQQLGLRE
LITRHYALPLQRTPSTLLLAVADPTNQQAEDDFRFATGLQVELVLADFRELTAAIRRLYGRSLNHEKSGLKEINQEELAN
LVDVGADEIDNIEDLSQDESPVSRYINQILLDAVRKGASDIHFEPYEKMYRVRLRCDGILIETQQPPSHLSRRLSARIKI
LSKLDIAERRLPQDGRIKLKLNQDTAIDMRVSTLPTLFGEKIVLRLLDSSSASLDIDKLGYSEQQKQLYLDALRRPQGMI
LMTGPTGSGKTVSLYTGLSILNKPEINISTAEDPVEINLSGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLDT
AEIAIKASQTGHLVLSTLHTNSAAETVIRLSNMGVESFNLASSLSLIIAQRLARKLCSHCKKSQELTVQLQHLGIQASDN
IFKANPDGCNECTHGYSGRTGIYEVMRFDEFLSEALIKGASVHELEKLAIANGMSTLQMSGIEKLKQGITSFSELQRVLY
F

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=407471 GPY59_RS22100 WP_158174152.1 2862164..2863849(+) (pilB) [Vibrio alginolyticus strain 2014V-1011]
ATGCTAACCAAACTCTCAACGGTTCTTCGCCAAAAAGGGTTACTAACATTTTCCCAAGAAGAATCGTTAATAGAGCAAGT
CAAAGCTTCTGGCATTTCGATGCCGGAAGCTTTACTCAGTTCTGGATTCTTCACGTCAAGTGAGCTCGCTGATCACTTAA
GTTCTCTCTTTGGTTTAAATCAGTCTGAGTTATCTCAGTATGAGTACGCTTCGCTTTGTCAGCAACTTGGCCTTAGGGAA
TTAATCACGCGGCATTACGCACTCCCACTCCAACGTACGCCTTCAACGTTATTATTAGCGGTTGCCGACCCCACGAACCA
ACAAGCAGAAGATGACTTTCGTTTTGCGACAGGGTTGCAAGTAGAACTTGTTCTCGCTGATTTTCGCGAGCTGACCGCTG
CGATTCGACGCTTGTATGGTCGTTCATTGAATCATGAGAAGTCTGGGTTAAAAGAGATAAACCAAGAAGAGTTAGCCAAC
CTGGTTGATGTGGGCGCAGATGAAATCGACAACATTGAAGACTTGAGCCAAGATGAGTCTCCTGTTAGCCGTTACATCAA
CCAAATTCTGTTGGACGCTGTGCGCAAAGGCGCATCCGATATCCACTTTGAGCCTTATGAAAAGATGTACCGCGTGCGCC
TACGCTGTGATGGCATCTTAATTGAAACACAGCAGCCACCAAGCCACTTAAGTCGCCGCTTATCCGCTCGTATCAAAATC
CTCTCTAAACTTGATATTGCAGAGCGACGTTTACCACAAGATGGGCGTATTAAGCTCAAGTTGAATCAAGACACCGCAAT
TGATATGCGTGTATCCACGCTACCGACTCTATTCGGAGAGAAAATTGTACTACGACTGCTCGACAGCAGCTCTGCGTCAC
TGGATATCGACAAGCTTGGTTATAGCGAACAACAAAAACAATTGTACTTGGATGCGCTGCGTCGCCCACAAGGCATGATT
CTGATGACAGGCCCAACAGGGAGCGGTAAAACCGTTTCTCTATACACTGGGCTAAGTATCCTCAATAAGCCAGAAATCAA
CATTTCCACGGCAGAAGACCCAGTAGAAATTAACTTATCGGGCATTAACCAAGTTCAAGTTCAGCCTAAGATTGGTTTTG
GTTTTGCCGAAGCCCTGCGCTCTTTCTTACGTCAAGACCCGGATGTGGTAATGGTCGGTGAGATTCGTGACTTAGATACC
GCCGAAATCGCTATAAAAGCATCGCAAACCGGTCACTTAGTACTTTCAACCTTACATACCAATTCTGCGGCTGAAACGGT
TATTCGTTTATCAAACATGGGCGTAGAAAGCTTTAACTTGGCCTCCTCTCTTAGTTTGATCATTGCCCAGCGCTTGGCCC
GTAAACTGTGCTCGCACTGTAAAAAATCACAAGAGCTTACCGTTCAGCTACAACATCTTGGAATTCAAGCAAGCGACAAC
ATTTTTAAAGCCAATCCCGATGGATGTAATGAGTGTACCCATGGTTATTCCGGCCGAACGGGGATTTATGAAGTGATGCG
CTTTGATGAATTTCTTTCCGAAGCGCTTATTAAAGGCGCCTCGGTGCATGAACTAGAAAAACTCGCCATCGCAAACGGGA
TGAGCACGTTACAAATGTCGGGCATAGAAAAACTCAAACAAGGGATCACAAGTTTTAGTGAACTACAGCGCGTACTTTAT
TTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio parahaemolyticus RIMD 2210633

95.187

100

0.952
  pilB Vibrio campbellii strain DS40M4

90.196

100

0.902
  pilB Vibrio cholerae strain A1552

74.199

100

0.743
  pilB Acinetobacter baumannii D1279779

51.036

94.652

0.483
  pilB Legionella pneumophila strain ERS1305867

49.168

96.435

0.474
  pilB Acinetobacter baylyi ADP1

50.097

92.157

0.462
  pilF Neisseria gonorrhoeae MS11

44.326

100

0.446
  pilB/pilB1 Synechocystis sp. PCC 6803

37.793

100

0.403
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.684

100

0.371
  pilF Thermus thermophilus HB27

36.477

100

0.365

Multiple sequence alignment