Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   GPY20_RS11650 Genome accession   NZ_CP046756
Coordinates   992534..994270 (+) Length   578 a.a.
NCBI ID   WP_233976036.1    Uniprot ID   -
Organism   Vibrio fluvialis strain 2015AW-0077     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 987534..999270
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY20_RS11630 pilM 989840..990844 (+) 1005 WP_158125470.1 type IV pilus assembly protein PilM Machinery gene
  GPY20_RS11635 pilN 990834..991424 (+) 591 WP_100267596.1 PilN domain-containing protein Machinery gene
  GPY20_RS11640 pilO 991417..992007 (+) 591 WP_020429291.1 type 4a pilus biogenesis protein PilO Machinery gene
  GPY20_RS11645 pilP 991997..992509 (+) 513 WP_020329446.1 pilus assembly protein PilP Machinery gene
  GPY20_RS11650 pilQ 992534..994270 (+) 1737 WP_233976036.1 type IV pilus secretin PilQ family protein Machinery gene
  GPY20_RS11655 aroK 994458..994976 (+) 519 WP_004728569.1 shikimate kinase AroK -
  GPY20_RS11660 aroB 995004..996089 (+) 1086 WP_158129945.1 3-dehydroquinate synthase -
  GPY20_RS11665 - 996135..997643 (+) 1509 WP_158129946.1 AAA family ATPase -
  GPY20_RS11670 - 997738..998568 (+) 831 WP_020329450.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62425.36 Da        Isoelectric Point: 4.5625

>NTDB_id=407241 GPY20_RS11650 WP_233976036.1 992534..994270(+) (pilQ) [Vibrio fluvialis strain 2015AW-0077]
MIKGLNASTAWGIRCLFVGACLLLSAATLAQESAANQLKNIDFRINKDKAAVLIVELASASAVVDVQKTEQGLNIELLKT
DVSDDKLYLLDVRDFATAVENIEVFRQQPSTLLVATVQGEFNYDYSLKGNYLEVVIRKLKAEEKAKPKSVLEKEGKLISI
NFQDIPVRNVLQLIADYNEFNLVVSDSVRGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPKEELDLREKQQLQ
KARMAEELGDLQSDILIINFAKASDIAQMIGGDGAVNMLSERGSISIDERTNALLIRELPENIAVIRDIVASLDIPVKQV
QIEARIVTVNEGNLDELGVRWGFSSTNGSTTTGGSIESNLGAIGLYDGGNNSGGSGDGVAIDDFLNVNLAATSANASSIA
FQVAKLGADTLLDLELSAMQRESKAEIISSPRLITTNKQPAYIEQGTEIPYLESSSSGATSVAFKKAVLSLKVTPQITPD
NRLVLDLSVTQDRPGEVVKTGTGEAVAINTQRIGTQVLVNNGETVVLGGIFQHSITNSVDKVPLLGDLPVLGALFRRSYE
QMGKSELLIFVTPKVVIQ

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=407241 GPY20_RS11650 WP_233976036.1 992534..994270(+) (pilQ) [Vibrio fluvialis strain 2015AW-0077]
ATGATTAAAGGACTAAATGCCTCCACGGCATGGGGAATACGTTGTCTGTTTGTGGGCGCATGCCTGCTGTTATCTGCCGC
CACACTTGCTCAGGAGTCGGCGGCCAATCAGCTGAAGAATATCGATTTTCGCATCAACAAAGACAAAGCGGCGGTACTGA
TTGTCGAACTGGCTTCCGCCTCGGCCGTGGTGGATGTGCAGAAAACCGAGCAAGGCCTGAATATCGAACTGCTGAAAACC
GATGTCAGCGATGACAAACTCTACCTGCTCGATGTGCGCGACTTTGCCACCGCAGTGGAAAACATCGAAGTGTTCCGCCA
GCAGCCCAGCACCTTGTTGGTCGCAACGGTGCAGGGGGAGTTCAATTATGATTACAGCCTGAAGGGTAACTATCTGGAAG
TGGTGATCCGCAAGCTAAAAGCCGAGGAGAAAGCCAAACCCAAAAGCGTACTAGAAAAAGAAGGCAAGCTGATTTCGATT
AACTTTCAGGATATTCCGGTGCGCAATGTGCTGCAGTTGATCGCCGACTACAATGAGTTCAATCTGGTGGTTTCCGATTC
AGTGCGCGGCAACCTGACGCTGCGCCTTGATGGCGTGCCCTGGCAGCAGGTGCTGGACATTATTCTGCAAGTCAAAGGTC
TGGATAAGCGCGTCGACGGCAATGTGATTCTGGTGGCGCCCAAAGAAGAGCTGGATCTGCGTGAAAAGCAGCAGTTGCAA
AAAGCCCGCATGGCGGAAGAACTCGGCGATCTGCAATCGGATATTCTCATCATCAACTTTGCCAAAGCGTCTGACATTGC
GCAGATGATTGGCGGCGATGGGGCGGTCAATATGTTGTCGGAGCGTGGTTCGATCAGCATTGATGAACGCACCAATGCGC
TGCTGATTCGTGAACTGCCGGAGAACATTGCTGTGATTCGCGATATCGTGGCGTCGCTCGATATTCCTGTCAAGCAAGTA
CAGATTGAAGCGCGTATTGTGACGGTCAATGAAGGTAACTTGGATGAACTGGGTGTGCGTTGGGGTTTCAGCTCCACCAA
TGGCAGCACGACCACGGGTGGCTCGATTGAAAGCAATCTGGGGGCAATTGGTTTGTACGATGGCGGTAATAATAGTGGCG
GTTCTGGTGATGGCGTTGCGATTGATGATTTCCTCAATGTGAACCTCGCTGCCACCTCAGCCAATGCGTCCAGCATTGCG
TTTCAGGTTGCCAAGCTTGGCGCAGATACCTTGCTCGATCTTGAACTGTCAGCCATGCAACGCGAGTCAAAAGCGGAAAT
CATCTCCAGTCCGAGACTGATCACCACCAACAAGCAGCCGGCGTATATTGAGCAGGGTACTGAGATTCCGTACTTGGAAT
CCTCATCCAGTGGCGCCACCAGTGTGGCATTCAAGAAGGCAGTGCTTAGTCTCAAAGTGACGCCGCAAATCACCCCCGAT
AACCGTCTGGTGCTCGATCTGAGCGTCACTCAGGATCGGCCGGGTGAAGTGGTGAAAACCGGTACTGGCGAGGCGGTGGC
GATCAACACGCAGCGCATTGGTACACAGGTGCTGGTCAACAACGGTGAGACCGTGGTTCTGGGCGGCATTTTCCAGCACA
GTATTACGAATTCAGTCGACAAGGTGCCGCTGTTGGGTGACTTGCCTGTGTTGGGCGCTCTGTTCCGTCGCAGCTACGAA
CAAATGGGCAAAAGTGAGCTGCTGATCTTCGTAACACCGAAAGTTGTGATTCAGTAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(418-576)

Bacterial type II/III secretion system short domain (Secretin_N)

(258-319)

Secretin and TonB N terminus short domain (STN)

(180-228)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

81.693

100

0.818
  pilQ Vibrio cholerae strain A1552

81.693

100

0.818
  pilQ Vibrio campbellii strain DS40M4

79.053

94.983

0.751
  pilQ Legionella pneumophila strain Lp02

42.287

95.329

0.403
  pilQ Legionella pneumophila strain ERS1305867

42.287

95.329

0.403
  pilQ Pseudomonas aeruginosa PAK

40.541

96.021

0.389
  comQ Acinetobacter baylyi ADP1

37.801

100

0.381

Multiple sequence alignment