Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   GPY20_RS11630 Genome accession   NZ_CP046756
Coordinates   989840..990844 (+) Length   334 a.a.
NCBI ID   WP_158125470.1    Uniprot ID   -
Organism   Vibrio fluvialis strain 2015AW-0077     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 984840..995844
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY20_RS11610 - 984990..985253 (-) 264 WP_020429299.1 DUF3624 domain-containing protein -
  GPY20_RS11615 - 985382..986116 (-) 735 WP_020329437.1 glutathione peroxidase -
  GPY20_RS11620 oxyR 986260..987156 (+) 897 WP_020329438.1 DNA-binding transcriptional regulator OxyR -
  GPY20_RS11625 - 987217..989697 (-) 2481 WP_158129944.1 PBP1A family penicillin-binding protein -
  GPY20_RS11630 pilM 989840..990844 (+) 1005 WP_158125470.1 type IV pilus assembly protein PilM Machinery gene
  GPY20_RS11635 pilN 990834..991424 (+) 591 WP_100267596.1 PilN domain-containing protein Machinery gene
  GPY20_RS11640 pilO 991417..992007 (+) 591 WP_020429291.1 type 4a pilus biogenesis protein PilO Machinery gene
  GPY20_RS11645 pilP 991997..992509 (+) 513 WP_020329446.1 pilus assembly protein PilP Machinery gene
  GPY20_RS11650 pilQ 992534..994270 (+) 1737 WP_233976036.1 type IV pilus secretin PilQ family protein Machinery gene
  GPY20_RS11655 aroK 994458..994976 (+) 519 WP_004728569.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 334 a.a.        Molecular weight: 36871.18 Da        Isoelectric Point: 6.6593

>NTDB_id=407237 GPY20_RS11630 WP_158125470.1 989840..990844(+) (pilM) [Vibrio fluvialis strain 2015AW-0077]
MGKSLVTGVDIGHHSIKAVVLKPKGESYSLVGYQEIVVSEDIFADNHTLNYQKIVKKLKELKKGLPLFSRKVALAVPDNA
VISKVLQIDSELDASEQEFAVYQAFSHQSPLPIEELSLDFVVTGEKTLGRSSSLTYQVYATRREVIDNRLSAMSKAGFEP
LLFDMQIHGLLNIWQWVSRAQQRTDWMLVDVGFSQTSLCIDFADKTPFYKDIPLGTRQLVAATSGGNADLTSPQAHFIQD
LVERLARQIQLFLSLQGHQSLAGIWLSGGGAATEGLAQAISERLVLPCELFNPLAEFTCRASLRHHPQVDLQRFSTAAGL
ALRGLNWLENGHAA

Nucleotide


Download         Length: 1005 bp        

>NTDB_id=407237 GPY20_RS11630 WP_158125470.1 989840..990844(+) (pilM) [Vibrio fluvialis strain 2015AW-0077]
ATGGGTAAATCATTAGTTACAGGTGTCGATATTGGCCACCACAGTATTAAAGCGGTGGTTTTAAAACCGAAAGGCGAATC
GTATTCGCTGGTCGGTTACCAAGAGATAGTCGTATCCGAGGATATTTTCGCTGATAACCACACGCTGAATTATCAGAAAA
TTGTAAAGAAACTCAAAGAACTAAAAAAGGGATTACCGCTATTTAGTCGTAAAGTGGCGCTGGCTGTACCGGACAATGCA
GTAATAAGTAAGGTATTACAGATAGATAGCGAACTGGATGCCAGCGAACAAGAGTTCGCGGTTTATCAGGCTTTTTCCCA
TCAATCGCCGCTACCCATCGAAGAGTTGAGCCTGGATTTCGTTGTCACCGGAGAAAAAACACTGGGGCGCAGCAGTTCGT
TGACGTATCAGGTCTATGCAACCCGACGTGAAGTGATCGACAACCGTTTGTCTGCAATGAGCAAAGCGGGTTTTGAGCCG
CTGCTGTTTGATATGCAGATTCACGGCCTGCTCAATATCTGGCAATGGGTCAGCCGTGCTCAGCAACGCACCGATTGGAT
GCTGGTTGATGTCGGGTTCAGTCAAACCTCATTGTGCATCGACTTTGCCGATAAAACGCCGTTTTACAAAGACATTCCGT
TGGGAACCCGCCAACTGGTTGCGGCGACCTCCGGTGGTAACGCTGACCTTACCAGCCCGCAAGCACACTTCATTCAGGAT
TTGGTTGAGCGTCTGGCGCGCCAGATCCAGCTGTTTCTTTCTCTGCAAGGCCATCAGTCGCTGGCTGGTATCTGGCTATC
CGGTGGTGGCGCGGCAACGGAAGGTTTGGCGCAGGCGATTAGCGAACGTCTGGTTCTGCCTTGCGAGCTGTTTAACCCTC
TGGCGGAGTTTACTTGCCGCGCATCGCTACGCCACCATCCTCAGGTTGATTTGCAGCGTTTCAGCACTGCGGCAGGCTTG
GCGCTGCGCGGGCTCAATTGGCTGGAGAACGGCCATGCTGCATAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(237-324)

Type IV pilus assembly protein PilM; (PilM_2)

(8-219)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

65.68

100

0.665
  pilM Vibrio campbellii strain DS40M4

47.826

100

0.494

Multiple sequence alignment