Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   GPY20_RS11635 Genome accession   NZ_CP046756
Coordinates   990834..991424 (+) Length   196 a.a.
NCBI ID   WP_100267596.1    Uniprot ID   -
Organism   Vibrio fluvialis strain 2015AW-0077     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 985834..996424
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY20_RS11620 oxyR 986260..987156 (+) 897 WP_020329438.1 DNA-binding transcriptional regulator OxyR -
  GPY20_RS11625 - 987217..989697 (-) 2481 WP_158129944.1 PBP1A family penicillin-binding protein -
  GPY20_RS11630 pilM 989840..990844 (+) 1005 WP_158125470.1 type IV pilus assembly protein PilM Machinery gene
  GPY20_RS11635 pilN 990834..991424 (+) 591 WP_100267596.1 PilN domain-containing protein Machinery gene
  GPY20_RS11640 pilO 991417..992007 (+) 591 WP_020429291.1 type 4a pilus biogenesis protein PilO Machinery gene
  GPY20_RS11645 pilP 991997..992509 (+) 513 WP_020329446.1 pilus assembly protein PilP Machinery gene
  GPY20_RS11650 pilQ 992534..994270 (+) 1737 WP_233976036.1 type IV pilus secretin PilQ family protein Machinery gene
  GPY20_RS11655 aroK 994458..994976 (+) 519 WP_004728569.1 shikimate kinase AroK -
  GPY20_RS11660 aroB 995004..996089 (+) 1086 WP_158129945.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 196 a.a.        Molecular weight: 22258.64 Da        Isoelectric Point: 9.5537

>NTDB_id=407238 GPY20_RS11635 WP_100267596.1 990834..991424(+) (pilN) [Vibrio fluvialis strain 2015AW-0077]
MLHNINLIPWREAQREAHKRRFVGLCVLALMVALGVQWGAGFYLDQQTQAQQQRLAFLNQHIHQLDAQIAELKVTEQEHK
ALLTRLNIVESLQQKRNKTTEFMNLMPQLIPEGVYVDKIKMNGEEIEISGISDSTARLATMLDNLEKSTQLSEVGMHSIV
SGNRRFGKQFQSFKVSFLFHASAHKPLATVTGEKHG

Nucleotide


Download         Length: 591 bp        

>NTDB_id=407238 GPY20_RS11635 WP_100267596.1 990834..991424(+) (pilN) [Vibrio fluvialis strain 2015AW-0077]
ATGCTGCATAACATCAACCTTATTCCCTGGCGGGAAGCGCAGCGCGAGGCGCACAAACGCCGCTTCGTCGGACTGTGCGT
GCTCGCGTTGATGGTGGCATTGGGCGTGCAGTGGGGCGCTGGCTTTTATCTCGATCAGCAAACTCAGGCTCAGCAGCAGC
GACTGGCATTTCTCAATCAGCACATTCACCAACTGGATGCGCAGATTGCCGAGCTGAAAGTGACCGAGCAAGAACATAAA
GCGCTGCTGACCCGGCTCAATATCGTCGAATCGTTACAGCAAAAGCGCAATAAGACCACAGAGTTCATGAACCTGATGCC
GCAGTTGATTCCGGAAGGTGTCTACGTCGACAAGATCAAAATGAACGGCGAAGAAATTGAAATCAGTGGCATCAGTGACA
GTACTGCCCGACTCGCCACCATGCTCGACAATCTGGAAAAGTCCACCCAACTGTCTGAGGTCGGTATGCATTCGATCGTG
TCAGGTAATCGACGTTTTGGTAAACAATTTCAGAGCTTTAAGGTGTCATTTCTGTTTCATGCTTCAGCTCATAAACCGCT
CGCCACCGTGACAGGAGAGAAACATGGCTAA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(103-176)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio cholerae strain A1552

69.149

95.918

0.663
  pilN Vibrio campbellii strain DS40M4

61.735

100

0.617

Multiple sequence alignment