Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   GPX93_RS10855 Genome accession   NZ_CP046749
Coordinates   2398403..2399629 (+) Length   408 a.a.
NCBI ID   WP_000648506.1    Uniprot ID   A0A0E3W4T9
Organism   Vibrio cholerae strain 2015V-1118     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2393403..2404629
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPX93_RS10830 fldB 2393639..2394157 (+) 519 WP_000690112.1 flavodoxin FldB -
  GPX93_RS10835 ampD 2394273..2394818 (-) 546 WP_053033235.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GPX93_RS10840 nadC 2395120..2396010 (+) 891 WP_123162947.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GPX93_RS10845 pilA 2396255..2396665 (+) 411 WP_123162948.1 pilin Machinery gene
  GPX93_RS10850 pilB 2396672..2398360 (+) 1689 WP_123162949.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GPX93_RS10855 pilC 2398403..2399629 (+) 1227 WP_000648506.1 type II secretion system F family protein Machinery gene
  GPX93_RS10860 pilD 2399687..2400562 (+) 876 WP_000418747.1 A24 family peptidase Machinery gene
  GPX93_RS10865 coaE 2400559..2401167 (+) 609 WP_184482901.1 dephospho-CoA kinase -
  GPX93_RS10870 zapD 2401199..2401939 (+) 741 WP_000207194.1 cell division protein ZapD -
  GPX93_RS10875 yacG 2402097..2402294 (+) 198 WP_000162865.1 DNA gyrase inhibitor YacG -
  GPX93_RS10880 parC 2402343..2404628 (-) 2286 WP_123162951.1 DNA topoisomerase IV subunit A -

Sequence

Protein


Download         Length: 408 a.a.        Molecular weight: 45090.38 Da        Isoelectric Point: 10.3424

>NTDB_id=407109 GPX93_RS10855 WP_000648506.1 2398403..2399629(+) (pilC) [Vibrio cholerae strain 2015V-1118]
MKATQTLPLKNYRWKGINSNGKKVSGQMLAISEIEVRDKLKDQHIQIKKLKKGSVSLLARLTHRVKGKDITILTRQLATM
LTTGVPIVQALKLVGDNHRKAEMKSILAQITKSVEAGTPLSKAMRTASAHFDTLYVDLVETGEMSGNLPEVFERLATYRE
KSEQLRAKVIKALIYPSMVVLVALGVSYLMLTMVIPEFESMFKGFGAELPWFTQQVLKLSHWVQAYSLWAFIAIAAAIFG
LKALRKNSFQIRLKTSRLGLKFPIIGNVLAKASIAKFSRTLATSFAAGIPILASLKTTAKTSGNVHFETAINEVYRDTAA
GMPMYIAMRNTDAFPEMVLQMVMIGEESGQLDDMLNKVATIYEFEVDNTVDNLGKILEPLIIVFLGTVVGGLVVAMYLPI
FNLMSVLG

Nucleotide


Download         Length: 1227 bp        

>NTDB_id=407109 GPX93_RS10855 WP_000648506.1 2398403..2399629(+) (pilC) [Vibrio cholerae strain 2015V-1118]
ATGAAAGCGACCCAAACCTTACCTCTGAAAAATTATCGCTGGAAAGGCATCAACAGCAACGGCAAAAAAGTTTCCGGCCA
GATGCTCGCCATCTCCGAAATCGAGGTGCGCGATAAGCTCAAAGATCAGCATATTCAGATCAAAAAACTGAAAAAAGGCA
GTGTATCTCTCTTGGCGCGCCTAACCCATCGCGTGAAAGGCAAAGACATCACGATTTTGACTCGGCAGTTGGCGACCATG
CTCACCACGGGCGTACCGATTGTGCAAGCCCTCAAGTTGGTGGGCGATAATCACCGTAAAGCTGAGATGAAATCGATTCT
GGCGCAAATCACCAAAAGCGTGGAAGCGGGCACGCCGCTTTCCAAGGCGATGCGCACCGCCAGCGCCCATTTTGATACCT
TGTATGTCGACTTAGTGGAAACCGGAGAGATGTCCGGTAACTTACCTGAGGTGTTTGAGCGTTTGGCCACCTACCGCGAG
AAAAGCGAGCAACTACGCGCCAAGGTGATTAAAGCGCTCATCTACCCCAGCATGGTGGTGTTGGTCGCGCTCGGGGTATC
TTACTTAATGCTCACCATGGTCATCCCAGAATTTGAAAGCATGTTTAAAGGCTTTGGTGCAGAACTGCCTTGGTTTACGC
AGCAAGTACTGAAACTCTCACACTGGGTACAGGCTTACAGTTTATGGGCATTTATCGCCATCGCAGCAGCCATTTTTGGC
TTGAAAGCGCTGCGTAAAAACTCTTTCCAGATCCGTTTAAAAACCAGCCGCTTAGGGCTGAAATTTCCGATTATTGGTAA
TGTGCTCGCTAAGGCTTCCATCGCCAAATTCAGCCGCACCCTCGCCACCAGCTTTGCCGCGGGGATCCCGATCCTCGCCA
GTTTAAAAACCACGGCCAAAACCTCCGGCAATGTGCACTTTGAAACCGCGATTAATGAGGTGTACCGCGATACCGCTGCG
GGTATGCCGATGTACATTGCCATGCGCAATACCGACGCTTTTCCCGAAATGGTGCTGCAAATGGTGATGATCGGTGAAGA
GTCAGGGCAATTAGACGACATGCTCAACAAGGTCGCGACCATCTATGAATTTGAAGTCGATAACACGGTCGATAACTTGG
GCAAGATTCTTGAACCACTGATCATCGTGTTTCTTGGGACGGTCGTGGGCGGCTTAGTGGTGGCGATGTACTTACCTATC
TTTAACTTAATGAGTGTATTGGGTTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(277-399)

Type II secretion system (T2SS), protein F (T2SSF)

(74-196)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0E3W4T9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio cholerae strain A1552

99.51

100

0.995
  pilC Vibrio campbellii strain DS40M4

74

98.039

0.725
  pilC Acinetobacter baumannii D1279779

42.647

100

0.426
  pilC Acinetobacter baylyi ADP1

42.015

99.755

0.419
  pilC Legionella pneumophila strain ERS1305867

42.211

97.549

0.412
  pilC Pseudomonas stutzeri DSM 10701

42.172

97.059

0.409
  pilG Neisseria gonorrhoeae MS11

41.045

98.529

0.404
  pilG Neisseria meningitidis 44/76-A

40.547

98.529

0.4

Multiple sequence alignment