Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   GPX93_RS10860 Genome accession   NZ_CP046749
Coordinates   2399687..2400562 (+) Length   291 a.a.
NCBI ID   WP_000418747.1    Uniprot ID   H9L4S3
Organism   Vibrio cholerae strain 2015V-1118     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2394687..2405562
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPX93_RS10840 nadC 2395120..2396010 (+) 891 WP_123162947.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GPX93_RS10845 pilA 2396255..2396665 (+) 411 WP_123162948.1 pilin Machinery gene
  GPX93_RS10850 pilB 2396672..2398360 (+) 1689 WP_123162949.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GPX93_RS10855 pilC 2398403..2399629 (+) 1227 WP_000648506.1 type II secretion system F family protein Machinery gene
  GPX93_RS10860 pilD 2399687..2400562 (+) 876 WP_000418747.1 A24 family peptidase Machinery gene
  GPX93_RS10865 coaE 2400559..2401167 (+) 609 WP_184482901.1 dephospho-CoA kinase -
  GPX93_RS10870 zapD 2401199..2401939 (+) 741 WP_000207194.1 cell division protein ZapD -
  GPX93_RS10875 yacG 2402097..2402294 (+) 198 WP_000162865.1 DNA gyrase inhibitor YacG -
  GPX93_RS10880 parC 2402343..2404628 (-) 2286 WP_123162951.1 DNA topoisomerase IV subunit A -

Sequence

Protein


Download         Length: 291 a.a.        Molecular weight: 32433.69 Da        Isoelectric Point: 7.1618

>NTDB_id=407110 GPX93_RS10860 WP_000418747.1 2399687..2400562(+) (pilD) [Vibrio cholerae strain 2015V-1118]
MELFYFYPWLFPVLATLFGLIVGSFLNVVIYRLPKIMEREWRAECAASFPEYGITPPEGKLTLSLPRSTCPHCQTPIRVI
DNIPLLSWLALRGQCSHCKAPISARYPLIELLTALMSLVIATHFPFGVFAVALLFFSYVLIAATFIDFDTLLLPDQLTLP
LLWGGIALALLGFSPVSLSDAVIGAMAGYLSLWSIYWLFKLLTGKEGMGYGDFKLLAALGAWLGWQQLPVIVLLSSVVGV
IFGLIQLRQQKKGIDMAFPFGPYLAIAGWFALLWGDKVIDWYFTTWVGQPL

Nucleotide


Download         Length: 876 bp        

>NTDB_id=407110 GPX93_RS10860 WP_000418747.1 2399687..2400562(+) (pilD) [Vibrio cholerae strain 2015V-1118]
ATGGAACTGTTTTATTTCTACCCTTGGCTGTTTCCGGTGCTCGCCACGCTGTTTGGCTTGATTGTCGGCAGCTTTCTCAA
TGTGGTGATCTACCGCTTACCCAAAATCATGGAGCGTGAATGGCGCGCGGAATGTGCGGCGAGTTTTCCTGAATATGGTA
TTACGCCACCAGAGGGTAAACTGACCCTCAGTTTGCCGCGTTCCACTTGCCCACACTGCCAAACGCCGATTCGCGTCATT
GATAATATTCCGCTACTGAGTTGGCTGGCGCTGCGCGGTCAATGCTCACACTGTAAAGCGCCGATCAGTGCTCGCTATCC
CTTGATTGAGCTACTCACTGCCCTCATGAGCTTAGTGATTGCCACTCACTTTCCGTTCGGAGTGTTTGCCGTGGCGCTGC
TGTTTTTCAGCTATGTATTGATTGCTGCAACCTTCATCGACTTCGATACCCTGTTACTGCCCGACCAATTAACGCTACCT
TTACTGTGGGGCGGTATTGCACTGGCACTGCTTGGTTTTTCTCCTGTTTCACTCAGTGATGCGGTGATCGGAGCCATGGC
GGGCTATCTCTCGCTGTGGTCGATTTACTGGCTATTTAAACTGCTGACAGGTAAAGAAGGCATGGGTTATGGTGATTTCA
AATTGCTCGCCGCACTCGGCGCTTGGCTAGGTTGGCAGCAGTTGCCTGTCATCGTACTGCTCTCATCGGTGGTTGGGGTG
ATTTTCGGTTTAATCCAACTGCGCCAGCAGAAAAAAGGTATTGATATGGCCTTTCCGTTTGGCCCTTATCTCGCCATCGC
GGGGTGGTTTGCCTTGCTGTGGGGCGATAAGGTAATTGATTGGTACTTCACCACTTGGGTAGGACAACCGTTATGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(17-124)

Type IV leader peptidase family (Peptidase_A24)

(135-244)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB H9L4S3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

100

100

1
  pilD Vibrio campbellii strain DS40M4

75.347

98.969

0.746
  pilD Acinetobacter nosocomialis M2

53.676

93.471

0.502
  pilD Acinetobacter baumannii D1279779

53.309

93.471

0.498
  pilD Neisseria gonorrhoeae MS11

44.792

98.969

0.443

Multiple sequence alignment