Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   GPW86_RS10415 Genome accession   NZ_CP046742
Coordinates   1012249..1013475 (+) Length   408 a.a.
NCBI ID   WP_114774363.1    Uniprot ID   -
Organism   Vibrio cholerae strain 3523-03     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1007249..1018475
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPW86_RS10395 ampD 1008059..1008604 (-) 546 WP_000567321.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GPW86_RS10400 nadC 1008900..1009790 (+) 891 WP_123011144.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GPW86_RS10405 pilA 1010035..1010511 (+) 477 WP_095473243.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  GPW86_RS10410 pilB 1010518..1012206 (+) 1689 WP_123011145.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GPW86_RS10415 pilC 1012249..1013475 (+) 1227 WP_114774363.1 type II secretion system F family protein Machinery gene
  GPW86_RS10420 pilD 1013533..1014408 (+) 876 WP_000418747.1 A24 family peptidase Machinery gene
  GPW86_RS10425 coaE 1014405..1015013 (+) 609 WP_184476127.1 dephospho-CoA kinase -
  GPW86_RS10430 zapD 1015045..1015785 (+) 741 WP_123011147.1 cell division protein ZapD -
  GPW86_RS10435 yacG 1015943..1016140 (+) 198 WP_000162865.1 DNA gyrase inhibitor YacG -
  GPW86_RS10440 parC 1016189..1018474 (-) 2286 WP_000102510.1 DNA topoisomerase IV subunit A -

Sequence

Protein


Download         Length: 408 a.a.        Molecular weight: 45110.37 Da        Isoelectric Point: 10.3424

>NTDB_id=406956 GPW86_RS10415 WP_114774363.1 1012249..1013475(+) (pilC) [Vibrio cholerae strain 3523-03]
MKATQTLSLKNYRWKGINSNGKKVSGQMLAISEIEVRDKLKDQHIQIKKLKKGSVSLLARLTHRVKSKDITILTRQLATM
LTTGVPIVQALKLVGDNHRKAEMKSILAQITKSVEAGTPLSKAMRTASAHFDTLYVDLVETGEMSGNLPEVFERLATYRE
KSEQLRAKVIKALIYPSMVVLVALGVSYLMLTMVIPEFESMFKGFGAELPWFTQQVLKLSHWVQAYSLWAFIAIAAAIFG
LKALRKNSFQIRLKTSRLGLKFPIIGNVLAKASIAKFSRTLATSFAAGIPILASLKTTAKTSGNVHFETAINEVYRDTAA
GMPMYIAMRNTDAFPEMVLQMVMIGEESGQLDDMLNKVATIYEFEVDNTVDNLGKILEPLIIVFLGTVVGGLVVAMYLPI
FNLMSVLG

Nucleotide


Download         Length: 1227 bp        

>NTDB_id=406956 GPW86_RS10415 WP_114774363.1 1012249..1013475(+) (pilC) [Vibrio cholerae strain 3523-03]
ATGAAAGCGACCCAAACCTTATCTCTGAAAAATTATCGCTGGAAAGGCATCAACAGCAACGGCAAAAAAGTTTCCGGCCA
GATGCTCGCCATCTCCGAAATCGAGGTGCGCGATAAACTCAAAGATCAGCATATTCAGATCAAAAAACTCAAAAAAGGCA
GTGTCTCTTTATTGGCACGCCTAACCCATCGCGTGAAAAGTAAAGATATTACGATTTTGACTCGGCAGTTGGCGACCATG
CTCACAACGGGCGTACCGATTGTGCAAGCCCTCAAGTTGGTGGGCGATAATCACCGTAAAGCTGAGATGAAATCGATTCT
GGCGCAAATCACCAAAAGTGTGGAAGCGGGCACGCCGCTTTCCAAGGCGATGCGCACCGCCAGCGCCCATTTTGATACCT
TGTATGTCGACTTAGTGGAAACCGGAGAGATGTCCGGTAACTTACCTGAAGTGTTTGAGCGTTTGGCCACCTACCGCGAG
AAAAGCGAGCAGCTACGCGCCAAGGTGATCAAAGCGCTCATCTACCCCAGCATGGTGGTGTTGGTCGCGCTCGGAGTTTC
CTACTTAATGCTCACCATGGTCATCCCAGAATTTGAAAGCATGTTTAAAGGCTTTGGTGCTGAACTGCCTTGGTTTACGC
AGCAAGTGCTGAAACTCTCACACTGGGTGCAGGCTTACAGTTTATGGGCATTTATCGCCATCGCAGCAGCCATTTTTGGC
TTGAAAGCGCTGCGTAAAAACTCCTTCCAGATCCGGTTGAAAACCAGTCGCTTGGGGCTGAAATTTCCGATTATTGGCAA
TGTACTCGCCAAAGCGTCCATCGCCAAATTCAGCCGCACCCTCGCCACCAGCTTTGCCGCGGGGATCCCGATCCTCGCCA
GTTTAAAAACCACGGCCAAAACCTCCGGCAATGTGCACTTTGAAACCGCGATTAATGAGGTGTACCGCGATACCGCTGCG
GGTATGCCGATGTACATTGCCATGCGCAATACCGACGCTTTTCCCGAAATGGTGCTGCAAATGGTGATGATCGGTGAAGA
GTCTGGGCAATTAGATGACATGCTCAACAAGGTCGCGACCATCTATGAATTTGAAGTCGATAACACGGTCGATAACTTGG
GCAAGATTCTTGAACCACTGATCATCGTCTTTCTTGGGACGGTCGTGGGCGGCTTAGTGGTGGCGATGTACTTACCTATC
TTTAACTTAATGAGTGTATTGGGTTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(277-399)

Type II secretion system (T2SS), protein F (T2SSF)

(74-196)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio cholerae strain A1552

99.51

100

0.995
  pilC Vibrio campbellii strain DS40M4

73.75

98.039

0.723
  pilC Acinetobacter baumannii D1279779

42.75

98.039

0.419
  pilC Acinetobacter baylyi ADP1

41.667

100

0.417
  pilC Pseudomonas stutzeri DSM 10701

42.04

98.529

0.414
  pilC Legionella pneumophila strain ERS1305867

42.065

97.304

0.409
  pilG Neisseria gonorrhoeae MS11

41.045

98.529

0.404
  pilG Neisseria meningitidis 44/76-A

40.547

98.529

0.4

Multiple sequence alignment