Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   GPW86_RS10405 Genome accession   NZ_CP046742
Coordinates   1010035..1010511 (+) Length   158 a.a.
NCBI ID   WP_095473243.1    Uniprot ID   -
Organism   Vibrio cholerae strain 3523-03     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1005035..1015511
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPW86_RS10385 fldB 1005879..1006397 (+) 519 WP_000690112.1 flavodoxin FldB -
  GPW86_RS10390 - 1006535..1007923 (+) 1389 WP_123011143.1 IS4-like element ISVa18 family transposase -
  GPW86_RS10395 ampD 1008059..1008604 (-) 546 WP_000567321.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GPW86_RS10400 nadC 1008900..1009790 (+) 891 WP_123011144.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GPW86_RS10405 pilA 1010035..1010511 (+) 477 WP_095473243.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  GPW86_RS10410 pilB 1010518..1012206 (+) 1689 WP_123011145.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GPW86_RS10415 pilC 1012249..1013475 (+) 1227 WP_114774363.1 type II secretion system F family protein Machinery gene
  GPW86_RS10420 pilD 1013533..1014408 (+) 876 WP_000418747.1 A24 family peptidase Machinery gene
  GPW86_RS10425 coaE 1014405..1015013 (+) 609 WP_184476127.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 158 a.a.        Molecular weight: 16576.11 Da        Isoelectric Point: 7.6279

>NTDB_id=406954 GPW86_RS10405 WP_095473243.1 1010035..1010511(+) (pilA) [Vibrio cholerae strain 3523-03]
MKAYKNKLQQGFTLIELMIVVAIIGILAAFAVPAYQNYTKKATLSEFPKALAAAKLAVEVCAHENASNETTFISNCVSGN
NGVPASFTLNNIDIQVITAANAGVSGAVAVEAKAKIAKGPIAQDESFIMAAVYKPEGLEWKSSCKDAAGKAQTSYCPD

Nucleotide


Download         Length: 477 bp        

>NTDB_id=406954 GPW86_RS10405 WP_095473243.1 1010035..1010511(+) (pilA) [Vibrio cholerae strain 3523-03]
ATGAAAGCGTATAAAAACAAACTACAGCAAGGTTTTACCTTAATTGAATTGATGATTGTGGTAGCGATTATTGGCATTTT
GGCTGCCTTCGCCGTTCCGGCCTATCAAAACTATACAAAAAAAGCTACTTTATCTGAATTTCCAAAAGCACTAGCCGCAG
CCAAGTTAGCAGTCGAAGTATGCGCTCATGAGAATGCATCAAATGAAACAACCTTCATTTCAAATTGTGTTTCTGGCAAT
AATGGCGTTCCAGCGTCGTTTACACTAAACAATATCGACATTCAAGTAATAACAGCTGCCAATGCTGGCGTTTCAGGTGC
GGTGGCTGTTGAAGCCAAGGCAAAAATTGCTAAAGGCCCAATTGCCCAAGATGAAAGCTTCATTATGGCTGCTGTATATA
AGCCCGAAGGACTCGAATGGAAAAGTTCTTGTAAAGATGCAGCAGGTAAAGCTCAAACATCTTACTGCCCAGACTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio campbellii strain DS40M4

56.688

99.367

0.563
  pilA Haemophilus influenzae 86-028NP

41.844

89.241

0.373
  pilA Haemophilus influenzae Rd KW20

42.446

87.975

0.373

Multiple sequence alignment