Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GPW86_RS10410 Genome accession   NZ_CP046742
Coordinates   1010518..1012206 (+) Length   562 a.a.
NCBI ID   WP_123011145.1    Uniprot ID   -
Organism   Vibrio cholerae strain 3523-03     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1005518..1017206
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPW86_RS10385 fldB 1005879..1006397 (+) 519 WP_000690112.1 flavodoxin FldB -
  GPW86_RS10390 - 1006535..1007923 (+) 1389 WP_123011143.1 IS4-like element ISVa18 family transposase -
  GPW86_RS10395 ampD 1008059..1008604 (-) 546 WP_000567321.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GPW86_RS10400 nadC 1008900..1009790 (+) 891 WP_123011144.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GPW86_RS10405 pilA 1010035..1010511 (+) 477 WP_095473243.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  GPW86_RS10410 pilB 1010518..1012206 (+) 1689 WP_123011145.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GPW86_RS10415 pilC 1012249..1013475 (+) 1227 WP_114774363.1 type II secretion system F family protein Machinery gene
  GPW86_RS10420 pilD 1013533..1014408 (+) 876 WP_000418747.1 A24 family peptidase Machinery gene
  GPW86_RS10425 coaE 1014405..1015013 (+) 609 WP_184476127.1 dephospho-CoA kinase -
  GPW86_RS10430 zapD 1015045..1015785 (+) 741 WP_123011147.1 cell division protein ZapD -
  GPW86_RS10435 yacG 1015943..1016140 (+) 198 WP_000162865.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62034.13 Da        Isoelectric Point: 5.9020

>NTDB_id=406955 GPW86_RS10410 WP_123011145.1 1010518..1012206(+) (pilB) [Vibrio cholerae strain 3523-03]
MLTNLVAILRQAELISATQEQAVVTQVSASGTSVPEALLGLGIFHPQELTEQLSHIFGLPETDLSRYDYAPLCQQLGLRE
LITRYDALPIAKQGNLLLLAVSDPTLLQAEEEFRFATGLQVELALADHRALQAAIRRLYGRSIQGAANQGKEISQDELAN
LVKVSDDELQSIEDLSQDDSPVSRFINQVLLDAVRKGASDIHFEPYENQYRIRLRCDGILVETQQPASHLSRRLAARIKI
LSKLDIAERRLPQDGRIKLCLSRDTAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYNPQQKQLYLNALKRPQGMI
LMTGPTGSGKTVSLYTGLRILNTSQINISTAEDPVEINLSGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSAAETVIRLANMGVEPFNLASSLSLIIAQRLARRLCKHCKITVRPSALLQSQFAFQPNE
ILYEANAAGCNECTGGYSGRVGIYEVMAFNTELAEAIMQRASIHQIERLAKANGMQTLQESGLEKLREGITSFAELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=406955 GPW86_RS10410 WP_123011145.1 1010518..1012206(+) (pilB) [Vibrio cholerae strain 3523-03]
ATGCTCACCAACCTTGTTGCTATCTTGCGTCAGGCTGAATTAATCAGCGCAACGCAAGAACAAGCGGTGGTTACACAGGT
TAGCGCTTCGGGAACTTCGGTGCCCGAGGCGTTGCTTGGTTTAGGGATTTTTCACCCCCAAGAACTCACCGAACAACTGA
GCCATATTTTCGGTTTGCCGGAAACCGACCTTAGCCGCTACGACTACGCCCCTCTATGCCAACAGCTCGGCCTGCGTGAA
CTGATAACCCGCTACGATGCCTTACCGATTGCCAAGCAAGGCAATTTATTGCTGCTCGCGGTATCTGACCCCACCTTACT
GCAAGCCGAAGAAGAGTTTCGTTTTGCGACTGGGTTGCAAGTTGAACTGGCACTGGCCGATCACCGCGCGCTGCAAGCCG
CGATTCGCCGTTTGTATGGCAGATCTATTCAAGGCGCAGCCAACCAAGGGAAAGAGATCAGCCAAGATGAGCTCGCCAAT
CTGGTTAAAGTCAGTGATGACGAGCTGCAATCGATTGAAGATCTCAGCCAAGATGACTCTCCGGTTAGCCGCTTTATCAA
CCAAGTGCTGCTCGATGCGGTGCGCAAAGGCGCCTCCGATATTCATTTTGAGCCTTATGAAAACCAGTATCGGATCCGGC
TGCGCTGCGATGGCATCTTGGTGGAAACTCAGCAACCGGCTAGCCATTTAAGTCGCCGTTTAGCCGCGCGGATTAAAATT
CTTTCCAAATTAGATATTGCCGAGCGCCGTTTGCCTCAAGACGGGCGGATTAAACTGTGCCTAAGCCGCGATACCGCCAT
TGATATGCGTGTATCCACGCTTCCCACTTTATGGGGAGAAAAAATCGTGCTGCGCCTGCTCGATAGCAGCGCCGCCAATC
TGGATATTGATAAGCTCGGCTATAACCCGCAGCAAAAGCAGCTCTATCTCAACGCCCTCAAAAGACCGCAAGGGATGATT
TTAATGACCGGCCCCACCGGCAGCGGCAAAACCGTTTCGCTCTATACTGGGCTGCGCATTCTCAACACGTCACAAATCAA
TATCTCCACCGCGGAAGATCCGGTGGAAATTAACCTCTCTGGGATTAACCAAGTGCAAGTGCAGCCGAAAATCGGCTTTG
GCTTTGCCGAAGCGCTACGCTCGTTTCTGCGCCAAGACCCGGATGTAGTGATGGTCGGCGAAATACGCGATCTGGAAACT
GCAGAAATCGCGGTAAAAGCCGCGCAAACCGGCCACTTGGTGCTTTCTACCCTGCATACCAACTCGGCCGCTGAAACCGT
AATTCGTTTAGCCAATATGGGGGTGGAGCCGTTTAACCTCGCGTCATCACTCAGTTTAATCATCGCCCAACGCCTCGCGC
GCCGCTTGTGTAAACATTGCAAAATCACGGTGCGCCCTTCTGCCCTATTGCAAAGTCAATTTGCGTTTCAGCCCAATGAA
ATCTTGTATGAAGCCAATGCGGCGGGGTGTAACGAGTGTACGGGCGGATATTCAGGGCGCGTTGGGATCTATGAAGTGAT
GGCGTTTAATACCGAGCTGGCGGAGGCCATTATGCAACGCGCCAGCATTCATCAAATTGAACGTTTAGCCAAAGCCAATG
GCATGCAAACGTTGCAAGAGTCCGGCCTTGAAAAACTGCGCGAAGGCATCACCAGCTTTGCCGAGCTGCAGCGTGTGCTC
TATTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

98.932

100

0.989
  pilB Vibrio parahaemolyticus RIMD 2210633

73.843

100

0.738
  pilB Vibrio campbellii strain DS40M4

73.665

100

0.737
  pilB Legionella pneumophila strain ERS1305867

52.642

90.925

0.479
  pilB Acinetobacter baylyi ADP1

50.385

92.527

0.466
  pilB Acinetobacter baumannii D1279779

50.192

92.527

0.464
  pilF Neisseria gonorrhoeae MS11

45.73

100

0.457
  pilB/pilB1 Synechocystis sp. PCC 6803

36.755

100

0.395
  pilF Thermus thermophilus HB27

39.595

96.619

0.383
  pilB Haemophilus influenzae 86-028NP

39.469

93.772

0.37

Multiple sequence alignment