Detailed information
Bioinformatically predicted
Overview
| Name | pilB | Type | Machinery gene |
| Locus tag | GPW70_RS07410 | Genome accession | NZ_CP046740 |
| Coordinates | 518714..520402 (-) | Length | 562 a.a. |
| NCBI ID | WP_001888471.1 | Uniprot ID | - |
| Organism | Vibrio cholerae strain 3566-06 | ||
| Function | assembly of type IV pilus (predicted from homology) DNA binding and uptake |
||
Genomic Context
Location: 513714..525402
| Locus tag | Gene name | Coordinates (strand) | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| GPW70_RS07385 | yacG | 514796..514993 (-) | 198 | WP_000162865.1 | DNA gyrase inhibitor YacG | - |
| GPW70_RS07390 | zapD | 515135..515875 (-) | 741 | WP_000207194.1 | cell division protein ZapD | - |
| GPW70_RS07395 | coaE | 515907..516515 (-) | 609 | WP_000011553.1 | dephospho-CoA kinase | - |
| GPW70_RS07400 | pilD | 516512..517387 (-) | 876 | WP_000418753.1 | A24 family peptidase | Machinery gene |
| GPW70_RS07405 | pilC | 517445..518671 (-) | 1227 | WP_000648507.1 | type II secretion system F family protein | Machinery gene |
| GPW70_RS07410 | pilB | 518714..520402 (-) | 1689 | WP_001888471.1 | type IV-A pilus assembly ATPase PilB | Machinery gene |
| GPW70_RS07415 | pilA | 520406..520831 (-) | 426 | WP_032473121.1 | prepilin-type N-terminal cleavage/methylation domain-containing protein | Machinery gene |
| GPW70_RS07420 | nadC | 521076..521966 (-) | 891 | WP_001888470.1 | carboxylating nicotinate-nucleotide diphosphorylase | - |
| GPW70_RS07425 | ampD | 522264..522809 (+) | 546 | WP_000567317.1 | 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD | - |
| GPW70_RS07430 | - | 522853..524241 (-) | 1389 | WP_001959226.1 | IS4-like element ISVa18 family transposase | - |
| GPW70_RS07435 | fldB | 524406..524924 (-) | 519 | WP_000690112.1 | flavodoxin FldB | - |
Sequence
Protein
Download Length: 562 a.a. Molecular weight: 62086.24 Da Isoelectric Point: 6.0856
>NTDB_id=406912 GPW70_RS07410 WP_001888471.1 518714..520402(-) (pilB) [Vibrio cholerae strain 3566-06]
MLTNLVVILRQAELISATQEQAVVSQVSASGTSVPEALLGLGIFHPQELTEQLSHIFGLPETDLSRYDYAPLCQQLGLRE
LITRYDALPIAKQGNLLLLAVSDPTLLQAEEEFRFATGLQVELALADHRALQAAIRRLYGRSIQGAANQGKEISQDELAT
LVKVSDDELQSIEDLSQDDSPVSRFINQVLLDAVRKGASDIHFEPYENQYRIRLRCDGILVETQQPASHLSRRLAARIKI
LSKLDIAERRLPQDGRIKLRLSRDTAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYNPQQKQLYLNALKRPQGMI
LMTGPTGSGKTVSLYTGLRILNTSQINISTAEDPVEINLSGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSAAETVIRLANMGVEPFNLASSLSLIIAQRLARRLCKHCKIAVRPSALLQNQFAFQPNE
ILYEANTAGCNECTGGYSGRVGIYEVMAFNTELAEAIMQRASIHQIERLAKANGMLSLQESGLEKLREGITSFAELQRVL
YF
MLTNLVVILRQAELISATQEQAVVSQVSASGTSVPEALLGLGIFHPQELTEQLSHIFGLPETDLSRYDYAPLCQQLGLRE
LITRYDALPIAKQGNLLLLAVSDPTLLQAEEEFRFATGLQVELALADHRALQAAIRRLYGRSIQGAANQGKEISQDELAT
LVKVSDDELQSIEDLSQDDSPVSRFINQVLLDAVRKGASDIHFEPYENQYRIRLRCDGILVETQQPASHLSRRLAARIKI
LSKLDIAERRLPQDGRIKLRLSRDTAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYNPQQKQLYLNALKRPQGMI
LMTGPTGSGKTVSLYTGLRILNTSQINISTAEDPVEINLSGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSAAETVIRLANMGVEPFNLASSLSLIIAQRLARRLCKHCKIAVRPSALLQNQFAFQPNE
ILYEANTAGCNECTGGYSGRVGIYEVMAFNTELAEAIMQRASIHQIERLAKANGMLSLQESGLEKLREGITSFAELQRVL
YF
Nucleotide
Download Length: 1689 bp
>NTDB_id=406912 GPW70_RS07410 WP_001888471.1 518714..520402(-) (pilB) [Vibrio cholerae strain 3566-06]
ATGCTCACCAACCTTGTTGTTATCTTGCGTCAGGCTGAGTTAATCAGCGCGACGCAAGAACAAGCGGTGGTTAGCCAAGT
GAGCGCTTCGGGAACTTCGGTACCCGAAGCGCTGCTTGGTTTAGGGATTTTTCACCCCCAAGAACTGACTGAACAACTGA
GCCATATTTTCGGCTTGCCGGAAACCGACCTTAGCCGCTACGACTACGCCCCCCTGTGCCAACAGCTCGGGCTGCGTGAA
CTAATAACCCGCTACGATGCCTTGCCGATTGCCAAGCAAGGCAATTTATTGCTGCTCGCGGTCTCTGACCCGACCTTACT
GCAAGCCGAAGAAGAGTTTCGTTTTGCCACAGGATTACAAGTTGAACTGGCACTGGCCGATCACCGCGCGCTGCAAGCCG
CGATTCGCCGTTTGTATGGCCGATCGATTCAAGGCGCAGCCAACCAAGGGAAAGAGATCAGCCAAGATGAGCTCGCCACA
CTGGTTAAAGTCAGTGATGACGAGCTGCAATCGATCGAAGATCTCAGCCAAGATGACTCTCCGGTTAGCCGCTTTATCAA
CCAAGTGCTGCTCGATGCGGTGCGCAAAGGCGCCTCCGATATTCATTTTGAGCCGTATGAAAACCAGTATCGGATCCGCC
TGCGCTGCGATGGGATCTTGGTTGAAACTCAGCAACCGGCTAGCCATTTAAGTCGCCGTTTAGCCGCGCGGATTAAAATT
CTCTCCAAATTAGATATTGCCGAGCGCCGCTTGCCGCAAGACGGCCGGATTAAACTGCGCCTAAGCCGCGATACCGCGAT
TGATATGCGTGTATCCACGCTTCCCACTTTATGGGGCGAAAAAATCGTGCTGCGTCTGCTCGATAGCAGCGCCGCCAATC
TGGATATTGATAAGCTCGGCTATAACCCGCAGCAAAAGCAGCTCTACCTCAACGCCCTCAAAAGACCGCAAGGGATGATT
TTGATGACAGGCCCCACCGGCAGCGGCAAAACCGTTTCGCTCTATACTGGGCTGCGCATTCTTAACACGTCACAGATCAA
TATCTCCACCGCGGAAGATCCGGTGGAAATTAACCTCTCTGGGATTAACCAAGTGCAAGTGCAGCCGAAAATCGGCTTTG
GCTTTGCCGAAGCGCTACGCTCGTTTCTACGCCAAGATCCGGATGTGGTGATGGTCGGCGAAATACGCGATCTGGAAACC
GCAGAAATCGCGGTAAAAGCCGCGCAAACCGGCCACTTGGTGCTTTCCACCCTGCACACAAACTCGGCCGCCGAAACCGT
GATTCGCTTAGCCAATATGGGGGTGGAGCCGTTTAATCTGGCGTCATCCCTCAGTTTAATCATCGCCCAACGCCTCGCGC
GCCGCCTATGTAAACACTGCAAAATCGCGGTGCGCCCTTCCGCGCTATTGCAAAACCAATTTGCATTTCAGCCCAATGAA
ATCTTGTATGAAGCCAATACGGCGGGGTGCAACGAGTGTACGGGCGGCTATTCAGGTCGCGTTGGGATCTATGAAGTGAT
GGCGTTTAATACTGAGCTGGCGGAGGCCATTATGCAACGCGCCAGTATTCATCAAATTGAACGTTTAGCCAAAGCCAATG
GTATGTTGAGCTTGCAAGAGTCCGGCCTTGAAAAGCTGCGCGAAGGCATCACCAGCTTTGCCGAGCTGCAGCGTGTGCTC
TATTTTTAA
ATGCTCACCAACCTTGTTGTTATCTTGCGTCAGGCTGAGTTAATCAGCGCGACGCAAGAACAAGCGGTGGTTAGCCAAGT
GAGCGCTTCGGGAACTTCGGTACCCGAAGCGCTGCTTGGTTTAGGGATTTTTCACCCCCAAGAACTGACTGAACAACTGA
GCCATATTTTCGGCTTGCCGGAAACCGACCTTAGCCGCTACGACTACGCCCCCCTGTGCCAACAGCTCGGGCTGCGTGAA
CTAATAACCCGCTACGATGCCTTGCCGATTGCCAAGCAAGGCAATTTATTGCTGCTCGCGGTCTCTGACCCGACCTTACT
GCAAGCCGAAGAAGAGTTTCGTTTTGCCACAGGATTACAAGTTGAACTGGCACTGGCCGATCACCGCGCGCTGCAAGCCG
CGATTCGCCGTTTGTATGGCCGATCGATTCAAGGCGCAGCCAACCAAGGGAAAGAGATCAGCCAAGATGAGCTCGCCACA
CTGGTTAAAGTCAGTGATGACGAGCTGCAATCGATCGAAGATCTCAGCCAAGATGACTCTCCGGTTAGCCGCTTTATCAA
CCAAGTGCTGCTCGATGCGGTGCGCAAAGGCGCCTCCGATATTCATTTTGAGCCGTATGAAAACCAGTATCGGATCCGCC
TGCGCTGCGATGGGATCTTGGTTGAAACTCAGCAACCGGCTAGCCATTTAAGTCGCCGTTTAGCCGCGCGGATTAAAATT
CTCTCCAAATTAGATATTGCCGAGCGCCGCTTGCCGCAAGACGGCCGGATTAAACTGCGCCTAAGCCGCGATACCGCGAT
TGATATGCGTGTATCCACGCTTCCCACTTTATGGGGCGAAAAAATCGTGCTGCGTCTGCTCGATAGCAGCGCCGCCAATC
TGGATATTGATAAGCTCGGCTATAACCCGCAGCAAAAGCAGCTCTACCTCAACGCCCTCAAAAGACCGCAAGGGATGATT
TTGATGACAGGCCCCACCGGCAGCGGCAAAACCGTTTCGCTCTATACTGGGCTGCGCATTCTTAACACGTCACAGATCAA
TATCTCCACCGCGGAAGATCCGGTGGAAATTAACCTCTCTGGGATTAACCAAGTGCAAGTGCAGCCGAAAATCGGCTTTG
GCTTTGCCGAAGCGCTACGCTCGTTTCTACGCCAAGATCCGGATGTGGTGATGGTCGGCGAAATACGCGATCTGGAAACC
GCAGAAATCGCGGTAAAAGCCGCGCAAACCGGCCACTTGGTGCTTTCCACCCTGCACACAAACTCGGCCGCCGAAACCGT
GATTCGCTTAGCCAATATGGGGGTGGAGCCGTTTAATCTGGCGTCATCCCTCAGTTTAATCATCGCCCAACGCCTCGCGC
GCCGCCTATGTAAACACTGCAAAATCGCGGTGCGCCCTTCCGCGCTATTGCAAAACCAATTTGCATTTCAGCCCAATGAA
ATCTTGTATGAAGCCAATACGGCGGGGTGCAACGAGTGTACGGGCGGCTATTCAGGTCGCGTTGGGATCTATGAAGTGAT
GGCGTTTAATACTGAGCTGGCGGAGGCCATTATGCAACGCGCCAGTATTCATCAAATTGAACGTTTAGCCAAAGCCAATG
GTATGTTGAGCTTGCAAGAGTCCGGCCTTGAAAAGCTGCGCGAAGGCATCACCAGCTTTGCCGAGCTGCAGCGTGTGCTC
TATTTTTAA
3D structure
| Source | ID | Structure |
|---|
Similar proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| pilB | Vibrio cholerae strain A1552 |
98.043 |
100 |
0.98 |
| pilB | Vibrio parahaemolyticus RIMD 2210633 |
73.665 |
100 |
0.737 |
| pilB | Vibrio campbellii strain DS40M4 |
73.31 |
100 |
0.733 |
| pilB | Legionella pneumophila strain ERS1305867 |
52.838 |
90.925 |
0.48 |
| pilB | Acinetobacter baylyi ADP1 |
50.385 |
92.527 |
0.466 |
| pilB | Acinetobacter baumannii D1279779 |
50 |
92.883 |
0.464 |
| pilF | Neisseria gonorrhoeae MS11 |
46.099 |
100 |
0.463 |
| pilB/pilB1 | Synechocystis sp. PCC 6803 |
36.755 |
100 |
0.395 |
| pilF | Thermus thermophilus HB27 |
39.187 |
96.263 |
0.377 |
| pilB | Haemophilus influenzae 86-028NP |
39.006 |
93.06 |
0.363 |